Normal-temperature preservation antioxidant diluent for swine semen and preparation method thereof

A technology of storage at room temperature and diluent, which is applied in the field of diluent for storage of porcine semen at room temperature and its preparation, can solve the problems of short effective time of semen storage and high price, and achieve the promotion of healthy and sustainable development of the pig industry, low cost, and enhanced resistance The effect of oxidative capacity

Inactive Publication Date: 2019-10-15
NORTHWEST A & F UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the defects of high price and short effective time of semen storage in existing diluents for storing porcine semen at room temperature, the p

Method used

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  • Normal-temperature preservation antioxidant diluent for swine semen and preparation method thereof
  • Normal-temperature preservation antioxidant diluent for swine semen and preparation method thereof
  • Normal-temperature preservation antioxidant diluent for swine semen and preparation method thereof

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Embodiment Construction

[0022] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments.

[0023] 1. Preparation of antioxidant diluent for porcine semen stored at room temperature

[0024] 1. Formula and preparation process

[0025] 1) The formula of the antioxidant diluent: every 1L of diluent contains 37.15g glucose, 1.25g ethylenediaminetetraacetic acid, 0.75g potassium chloride, 6.00g disodium citrate, 1.25g sodium bicarbonate, 0.25g Gentamicin and a certain quality of Radix isatidis polysaccharide.

[0026] 2) Weighing and dissolving of reagents: The diluent should be ready for use immediately. 2 hours before the semen dilution, accurately weigh the ingredients of the formula with an analytical balance, dissolve with double distilled water and make up to 1L.

[0027] 3) Filtration of the diluent: After the components in the diluent are fully dissolved, filter and sterilize with a 0.22 μm microporous membrane, and place it in...

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Abstract

The invention discloses a normal-temperature preservation antioxidant diluent for swine semen and a preparation method thereof. The diluent is prepared from glucose, EDTA, potassium chloride, disodiumcitrate, sodium bicarbonate, gentamicin and isatis root polysaccharide. The plant-derived natural antioxidant isatis root polysaccharide is used for replacing an artificially synthesized antioxidant,so that the antioxidant capacity of swine semen can be enhanced, oxidative damage can be relieved, the motility rate, plasma membrane integrity and acrosome integrity of the swine semen can be effectively maintained, and finally, the fertilization capacity of the semen can be maintained. The normal-temperature liquid storage time of the swine semen can be prolonged, and the requirements of pig production in remote areas are satisfied; meanwhile, the diluent is low in cost, simple to operate, stable in preservation effect and suitable for wide popularization. The technology has important significance for promoting healthy and sustainable development of the pig industry.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a diluent for storing porcine semen at room temperature and a preparation method thereof. Background technique [0002] At present, pig artificial insemination technology is usually used in large-scale farms to breed piglets. On the one hand, it can improve the utilization rate of boars, and on the other hand, it can accelerate the improvement of pig breeds. The key step in artificial insemination is semen preservation. However, there are a lot of unsaturated fatty acids in the plasma membrane of porcine sperm, which has low antioxidant capacity, making it more susceptible to oxidative damage during freezing and thawing than sperm from other species. At the same time, cryopreservation is complicated and costly, so the normal temperature storage of pig semen has gradually become the preferred method in pig production. [0003] The level of reactive oxygen species in fresh...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 庞卫军任志强邵勇维克王晨阳张悫杨公社
Owner NORTHWEST A & F UNIV
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