Kasugamycin residue protein united Chinese herbal medicine preparation for resisting WSSV (white spot syndrome virus) and application of kasugamycin residue protein united Chinese herbal medicine preparation

A technology of kasugamycin and Chinese herbal medicine, which is applied in the field of kasugamycin residue protein combined with Chinese herbal medicine preparations, can solve the problem of insignificant drug efficacy, achieve the effects of controlling WSSV damage to prawns, improving product quality, survival rate and yield

Pending Publication Date: 2019-10-15
陕西麦可罗生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the problems existing in the prior art, the present invention provides a kind of anti-WSSV kasugamycin residue protein combined with Chinese herbal medicine preparation and application thereof, which overcomes the insignificant shortcoming of traditional Chinese herbal medicine for preventing WSSV, and on the other hand also provides Recycling of Kasugamycin Residues Provides New Ideas and Approaches

Method used

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  • Kasugamycin residue protein united Chinese herbal medicine preparation for resisting WSSV (white spot syndrome virus) and application of kasugamycin residue protein united Chinese herbal medicine preparation
  • Kasugamycin residue protein united Chinese herbal medicine preparation for resisting WSSV (white spot syndrome virus) and application of kasugamycin residue protein united Chinese herbal medicine preparation

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Experimental program
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Effect test

Embodiment 1

[0058] After the aforementioned steps of protein and polypeptide separation and purification, the protein of the residue is separated from other substances of the residue such as metal ions, lipids, sugars, and cellulose to obtain the kasugamycin residue protein. After testing, the Kasugamycin In mycin residue protein, by mass ratio:

[0059] 28.4kDa: 40-50%

[0060] 31.0kDa: 20-30%

[0061] 14.4kDa: 10-20%,

[0062] 58.9kDa: 10-20%.

[0063] The above-mentioned kasugamycin residue protein is regarded as the kasugamycin residue protein group.

[0064] The components of the Chinese herbal medicine group are: 10 parts of honeysuckle, 15 parts of forsythia, 25 parts of Radix Radix, 20 parts of Astragalus, 15 parts of Folium Folium, 10 parts of Cuscuta, and 5 parts of crude protein.

[0065] The composition of the kasugamycin residue protein and Chinese herbal medicine interworking group is: the above-mentioned kasugamycin residue protein and the above-mentioned Chinese herbal...

Embodiment 2

[0068] To determine the protein content of the kasugamycin residue, the following steps were used:

[0069] ①Preparation of standard protein solution: Accurately weigh 500mg of bovine serum albumin, dilute to 50mL with distilled water, and make 1L of 10mg / mol solution. Take 1mL of this solution and dilute it to 10mL with deionized water to prepare a 1.00mg / mL standard protein solution. ② Preparation of Coomassie Brilliant Blue G-250 solution: Accurately weigh 100 mg of Coomassie Brilliant Blue G-250, dissolve in 50 mL of 95% ethanol, add 120 mL of 85% phosphoric acid, dilute to 1000 mL for later use. ③Drawing of the standard curve: Dilute the standard protein (initial concentration: 1.00mg / mL) successively to 0, 0.2, 0.4, 0.6, 0.8 and 1.00mg / mL protein concentration. After mixing evenly, add 5mL test solution to each tube. Shake the Masie Brilliant Blue solution, and let it stand for about 5 minutes. Use a cuvette with a light path of 1 cm to measure the absorbance at 595 nm....

Embodiment 3

[0071] In order to compare the mortality of each group after WSSV injection in different feeding groups, several tails of Litopenaeus vannamei with typical symptoms of WSSV were selected from the WSSV shrimp pond, and WSSV was positive by PCR detection.

[0072] For diseased shrimp, remove the head, thorax and hepatopancreas, and cut them into pieces under ice bath conditions. Add 100m L of PBS buffer solution per gram of tissue, the pH of the buffer solution is about 7.6, homogenize in an ice bath for 1 hour, filter the tissue fluid through a 200-mesh silk screen through the homogenate, and then filter through a 0.45 μm microporous membrane, Obtain filtrate, and this filtrate just is free WSSV crude extraction mother liquor, then gets this mother liquor 1mL, dilutes this mother liquor with PBS damping fluid 10, 10 -4The diluent was intramuscular injection of prawns, and the experimental group was fed with Chinese herbal medicine for 7 days, fed with kasugamycin residue for 7 ...

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Abstract

The invention relates to a kasugamycin residue protein united Chinese herbal medicine preparation for resisting WSSV (white spot syndrome virus) and an application of the kasugamycin residue protein united Chinese herbal medicine preparation. Protein like polypeptide type substances are separated from kasugamycin residues, then the protein like polypeptide type substances, honeysuckle flowers, fructus forsythiae, radix isatidis, radix astragali, semen cuscutae and folium isatidis mutually cooperate, a mutual cooperation preparation for resisting white spot syndrome virus (white spot syndrome virus, WSSV) of prawns is prepared, and is used for preventing and treating the WSSV of the prawns. The cellular immunity factors of the prawns fed with kasugamycin residue protein united Chinese herbal medicines are respectively that THC is (3.83+/-0.129)*10<7>cells.mL<-1>, PO is (333+/-10.6)U.100mL<-1>, SOD is (383+/-10.9)U.100mL<-1>, ALP is (45.83+/-4.11)U.100mL<-1>, the THC content of a kasugamycin residue protein united Chinese herbal medicine feeding prawn group has significant differences (P is smaller than 0.05), and the PO value, the SOD value and the ALP value are notably higher thanthose in a control group (P is smaller than 0.05); and after the prawns are infected with the WSSV for 16d, the mortality rate of the prawns in the kasugamycin residue protein united Chinese herbal medicine feed group is the lowest.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation and aquaculture, and in particular relates to a WSSV-resistant kasugamycin residue protein combined with Chinese herbal medicine preparation and application thereof. Background technique [0002] Shrimp belongs to Arthropoda, Crustacea, Decapoda, Natantia, Penaeidae (Zhang Weiquan, 1990), and Penaeidae is the main species of aquaculture in the world. , which includes 6 genera and 29 species. Among them, Litopenaeus vannamei, Penaeus monodon and Fenneropenaeus chinensis are the three main species of prawns cultured in my country. Today, my country is the country with the highest shrimp production and the largest farming area in the world, followed by Thailand, Indonesia, Vietnam, India, Brazil, the Philippines, Ecuador, Mexico, etc. The coastal fish and shrimp aquaculture model in my country adopts an intensive and efficient farming model, such as shrimp Breeding mode High level pond ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/01A61P31/20A61K36/855A61K36/704A61K36/634
CPCA61K38/01A61K36/355A61K36/634A61K36/195A61K36/315A61K36/481A61K36/39A61K36/43A61K36/855A61K36/704A61P31/20A61K2236/331A61K2300/00
Inventor 潘忠成师维杨振飞邓子新李蒲民
Owner 陕西麦可罗生物科技有限公司
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