Panax japonicus var. major glycosyl transferase UGTPjm1 gene and application thereof in preparing ginsenoside Ro

A technology of ginsenoside and ginseng glycosyl, applied in the direction of transferase, application, genetic engineering, etc., can solve the problems of relatively harsh cultivation environment requirements, low yield of ginsenoside Ro, complex extraction process, etc., to achieve easy separation and purification, The effect of convenient management and single production product

Active Publication Date: 2019-10-18
YUNNAN AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0004] Ginsenoside Ro is mainly distributed in ginseng plants such as pearl ginseng, pearl joint ginseng, and ginger-shaped Panax notoginseng, and these plants have relatively harsh requirements on the cultivation environment, long growth cycle, and low yield; in addition, they also have a relatively complicated extraction process. , low separation yield and other deficiencies make the output of ginsenoside Ro low, resulting in high market prices

Method used

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  • Panax japonicus var. major glycosyl transferase UGTPjm1 gene and application thereof in preparing ginsenoside Ro
  • Panax japonicus var. major glycosyl transferase UGTPjm1 gene and application thereof in preparing ginsenoside Ro
  • Panax japonicus var. major glycosyl transferase UGTPjm1 gene and application thereof in preparing ginsenoside Ro

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Embodiment 1

[0035] Mining and in vitro verification of UGT candidate gene UGTPjm1

[0036] The enlarged rhizomes and slender stems of Ginseng ginseng were used as materials, and samples were taken respectively and sent to Guangzhou Kidio Biotechnology Co., Ltd. for transcriptome sequencing. First, according to the annotation results of transcriptome data, all 136 candidate glycosyltransferases were screened out. Secondly, it combines the characteristics of glycosyltransferase genes with known functions, including potato StSGT, cassia SaGT4A, soybean UGT73P2, licorice GuUGAT, etc. Then combined with the content distribution characteristics of ginsenoside Ro in the rhizomes of Ginseng ginseng and gene expression, 12 possible candidate genes were preliminarily screened out. After a series of work such as cloning, homologous recombination, prokaryotic expression, in vitro enzymatic reaction, high-performance liquid phase detection and LC / MC identification of the candidate gene, it was finall...

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Abstract

The present invention discloses a panax japonicus var. major glycosyl transferase UGTPjm1 gene and an application thereof in preparing ginsenoside Ro. A nucleotide sequence of the panax japonicus var.major glycosyl transferase UGTPjm1 gene is represented by SEQ ID NO:1. Panax japonicus saponin IVa and a glycosyl donor UDP-glucose are used as raw materials and a glucuronic acid group at a C3 position of the panax japonicus saponin IVa is once further glycosylated to form the ginsenoside Ro catalyzed by panax japonicus var. major glycosyl transferase encoded by the panax japonicus var. major glycosyl transferase UGTPjm1 gene. The biosynthesis regulation gene UGTPjm1 of the ginsenoside Ro is first identified and successfully verified. By synthesizing the ginsenoside Ro in vitro, management is more convenient, controllability is strong, demand for raw material planting can be reduced, agricultural land is saved, a production product is single, and separation and purification of the ginsenoside Ro in a late stage are facilitated. The panax japonicus var. major glycosyl transferase UGTPjm1 gene is used as a key gene for the biosynthesis of the ginsenoside Ro and can also be used for breeding studies of panax japonicus var. major and other plants.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a gene of ginseng glycosyltransferase UGTPjm1 and its application in preparing ginsenoside Ro. Background technique [0002] Pearl ginseng (Panax japonicus var. major) is a medicinal plant of the genus Panax of the family Araliaceae, and is used as medicine with rhizomes. At present, a variety of triterpene saponins have been isolated and identified from the rhizomes of Panax ginseng, the content of which is mainly oleanolic acid type saponins. [0003] Oleanolic acid type saponin is a kind of pentacyclic triterpene saponin widely existing in the plant kingdom. AHAs are more pronounced. Ginsenoside Ro is an oleanolic acid type saponin, and its content is relatively high in the rhizome of Pearl Ginseng. Ginsenoside Ro has various pharmacological effects such as anti-inflammation, regulating immunity, protecting cardiovascular system, anti-virus, protecting liver, protec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/10C12N15/54C12N15/70C12N1/21C12N15/11C12P33/20C12R1/19
CPCC12N9/1048C12N15/70C12P33/20
Inventor 杨生超唐军荣张广辉陈庚林源闫静李莹
Owner YUNNAN AGRICULTURAL UNIVERSITY
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