Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for inoculating sclerotinite to rapeseed field stem

A technology of sclerotinia and stalks, which is applied in the field of sclerotinia sclerotiorum inoculation in rapeseed fields, can solve the problems of plant damage, investigation, and unstable identification results, so as to ensure humidity and integrity, increase the success rate of inoculation, The effect of facilitating vaccination identification

Pending Publication Date: 2019-10-18
WUHAN ACADEMY OF AGRI SCI
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the field toothpick inoculation method and the stem mycelium block binding method are simple and easy to implement, and are suitable for large-scale field inoculation. However, this method is easily affected by field environmental conditions, especially low field humidity, high inoculation failure rate, and unstable identification results.
The in vitro stalk inoculation method needs to collect at least 10 individual stalks for greenhouse inoculation. Due to the irreversible damage to the plants, it is not conducive to the investigation of rapeseed yield and quality traits in the later stage, and is not suitable for large-scale inoculation.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for inoculating sclerotinite to rapeseed field stem
  • Method for inoculating sclerotinite to rapeseed field stem
  • Method for inoculating sclerotinite to rapeseed field stem

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1) Collect the caps of centrifuge tubes used for other experiments

[0029] Collect the discarded 1.5mL or 2.0mL centrifuge tubes generated in the laboratory for configuring PCR, DNA extraction, etc., cut off the cover of the entire centrifuge tube with scissors, wash off the surface impurities, and then soak in 84 for 1 hour and then rinse. Allow to dry fully.

[0030] 2) Preparation of PDA medium

[0031] Buy fresh potatoes, peel and remove the buds, weigh 200g of potatoes, cut them into pieces, add 1000mL ddH2O to boil on high heat, then boil on low heat for 30min, then filter the filtrate with two layers of wet gauze and add ddH2O to 900mL. Then add 20g of agar (not added when preparing liquid medium), 20g of glucose, stir well to make it dissolve, and then set the volume to 1000mL. The thickness is not less than 5mm.

[0032] 3) Cultivation and activation of Sclerotinia

[0033] Take out the preserved Sclerotinia strains from the refrigerator at 4°C, use a punc...

Embodiment 2

[0039] 1. Preparation of PDA medium

[0040] Buy fresh potatoes, peel and remove the buds, weigh 200g of potatoes, cut them into pieces, add 1000mL ddH2O to boil on high heat, then boil on low heat for 30min, then filter the filtrate with two layers of wet gauze and add ddH2O to 900mL. Then add 20g of agar (not added when preparing liquid medium), 20g of glucose, stir well to make it dissolve, and then set the volume to 1000mL. The thickness is not less than 5mm.

[0041] 2. Cultivation and activation of Sclerotinia

[0042] Take out the preserved Sclerotinia strains from the refrigerator at 4°C, use a puncher to take out a culture medium containing mycelium blocks with a diameter of 5mm, place it on the prepared PDA medium plate, seal the culture dish and turn it upside down to prevent The water vapor in the medium affects the germination of mycelium. Culture in dark light at 22°C until the mycelium grows to the edge of the petri dish. Take the vigorously growing mycelium o...

Embodiment 3

[0046] a. Preparation of PDA medium

[0047] Buy fresh potatoes, peel and remove the buds, weigh 200g of potatoes, cut them into pieces, add 1000mL ddH2O to boil on high heat, then boil on low heat for 30min, then filter the filtrate with two layers of wet gauze and add ddH2O to 900mL. Then add 20g of agar (not added when preparing liquid medium), 20g of glucose, stir well to make it dissolve, and then set the volume to 1000mL. The thickness is not less than 5mm.

[0048] b. Cultivation and activation of Sclerotinia

[0049] Take out the preserved Sclerotinia strains from the refrigerator at 4°C, use a puncher to take out a culture medium containing mycelium blocks with a diameter of 5mm, place it on the prepared PDA medium plate, seal the culture dish and turn it upside down to prevent The water vapor in the medium affects the germination of mycelium. Culture in dark light at 22°C until the mycelium grows to the edge of the petri dish. Take the vigorously growing mycelium o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of plant disease-resistance identification, and provides a method for inoculating sclerotinite to a rapeseed field stem. The method includes the followingsteps: collecting a centrifuge tube cover, preparing a mycelia module, field inoculating, and phenotypic observation statistics. The method utilizes the centrifuge tube cover to moisturize an agar block containing the mycelia, further moisturizes and fixes with a preservative film, and does not need to carry out artificial spraying for 7 days after the inoculation to maintain the field humidity topromote the disease. The method is simple and reliable, and is convenient for large-scale inoculation in the field; the inoculation efficiency is high, the success rate is high, and the result is stable; can effectively ensure the humidity and disease conditions of the mycelial block after field inoculation, and is suitable for large-scale inoculation identification of materials.

Description

technical field [0001] The invention belongs to the technical field of identification of plant disease resistance, and in particular relates to a method for inoculating sclerotinia sclerotiorum on rapeseed field stalks. Background technique [0002] Sclerotinia sclerotiorum is a fungal disease caused by Sclerotinia sclerotiorum. During the growth of rapeseed, Sclerotinia sclerotiorum is one of the most important diseases that affect the yield of rapeseed. The annual yield loss caused by Sclerotinia sclerotiorum is more than 80%. Breeding anti-sclerotinia varieties is the most effective way to control Sclerotinia. At present, the identification method that can best reflect the resistance to Sclerotinia sclerotiorum is to conduct stalk inoculation identification at the adult plant stage of rapeseed. The stem inoculation methods at the adult stage include the field toothpick inoculation method and the isolated stem inoculation method. Among them, the field toothpick inoculat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/24
CPCC12Q1/24
Inventor 王转茸万丽丽杨光圣
Owner WUHAN ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com