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Oral membrane for reconstruction of attached gingivae, and preparation method thereof

An oral membrane and attached gingiva technology, which is applied in the field of reconstructed oral membranes with attached gingiva and its preparation, can solve the problems of affecting the reconstruction of attached gingiva, impair penetration, etc., and achieve the effects of low immunity, promotion of cell proliferation, and no rejection reaction.

Active Publication Date: 2019-10-25
山东奥精生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patented composition for periodontal soft tissue regeneration and its preparation method (publication number CN 101472622A) points out that the substrate matrix material is (1) hyaluronic acid and its derivatives (2) collagen and its derivatives (3) blood fiber Protein and fibrinogen (4) One or more of plasma and platelets mixed with mesenchymal stem cells or gingival fibroblasts are injected into the gingival recession area, which can well promote the attachment of gingiva and The regeneration of the interdental papilla requires a certain degree of fluidity due to the injection method, but too much or too little matrix material will impair the penetration into the tissue during injection, resulting in easy formation of the shape and shape of the injection site with tiny protrusions and depressions. tissue, affecting the reconstruction of attached gingiva

Method used

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  • Oral membrane for reconstruction of attached gingivae, and preparation method thereof
  • Oral membrane for reconstruction of attached gingivae, and preparation method thereof
  • Oral membrane for reconstruction of attached gingivae, and preparation method thereof

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preparation example Construction

[0048] The present invention provides a method for preparing oral membranes for attached gingiva reconstruction, referring to figure 1 , the preparation method comprises the steps of:

[0049] (1) Dissolving atelopeptide type I collagen in acid solution to prepare collagen acid solution;

[0050] (2) defoaming and freeze-drying the collagen acid solution to obtain a sponge;

[0051] (3) The sponge is rolled, and the temperature of the roller is set at 30-40°C, more preferably at 35±2°C, to obtain the oral membrane.

[0052] In the present invention, the collagen sponge obtained after freeze-drying is rolled, and the temperature of the rollers is at 30 to 40°C (for example, 30°C, 31°C, 32°C, 33°C, 34°C, 35°C) during the controlled rolling. , 36°C, 37°C, 38°C, 39°C, 40°C, more preferably 35±2°C) the sponge can be pressed into a film, and the upper and lower surfaces of the film are smooth. The inventor found in research that if the temperature during rolling is too high (the te...

Embodiment 1

[0070] Step S-1: remove the fat and fascia on the Achilles tendon, wash and freeze;

[0071] Step S-2: cutting the frozen Achilles tendon into thin slices, the thickness of which is controlled at 0.8-1.0 mm;

[0072] Step S-3: Soak the slices cut in S-2 in 0.8wt% sodium bicarbonate solution for 24 hours for degreasing, and then wash them with purified water;

[0073] Step S-4: adding the degreased flakes to an acetic acid solution with a pH of 1, adding pepsin, and enzymatically hydrolyzing at 0° C. for 72 hours to remove telopeptides;

[0074] Step S-5: Centrifuge the enzymolysis solution obtained in step S-4 with a centrifuge, take the supernatant, and separate out collagen flocs with a sodium chloride solution;

[0075] Step S-6: Put the collagen flocs obtained in Step S-5 into a dialysis bag for dialysis, use gradient dialysis to perform dialysis, gradually reduce the concentration of acetic acid in the external fluid, and finally use purified water dialysis to complete t...

Embodiment 2

[0106] Step S-1: remove the fat and fascia on the Achilles tendon, wash and freeze;

[0107] Step S-2: cutting the frozen Achilles tendon into thin slices, the thickness of which is controlled at 0.8-1.0 mm;

[0108] Step S-3: Soak the slices cut in S-2 in 0.9wt% sodium bicarbonate solution for 18 hours for degreasing, and then wash them with purified water;

[0109] Step S-4: adding the defatted flakes to a citric acid solution with a pH of 3, adding pepsin, and enzymatically hydrolyzing at 14° C. for 72 hours to remove telopeptides;

[0110] Step S-5: Centrifuge the enzymolysis solution obtained in step S-4 with a centrifuge, take the supernatant, and use sodium carbonate solution to separate out collagen flocs;

[0111] Step S-6: Put the collagen flocs obtained in Step S-5 into a dialysis bag for dialysis, use gradient dialysis to perform dialysis, gradually reduce the concentration of acetic acid in the external fluid, and finally use purified water dialysis to complete t...

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Abstract

The invention relates to an oral membrane for reconstruction of attached gingivae, and a preparation method thereof. The preparation method includes (1) dissolving type-I atelocollagen in an acid solution to prepare a collagen acid solution; (2) defoaming and freeze-drying the collagen acid solution to obtain sponge; (3) rolling the sponge to obtain the oral membrane, wherein the temperature of aroller is set as 30-40 DEG C. A compact and smooth film is formed on the surface of the oral membrane prepared by the preparation method, the mechanical properties are improved, and the oral membranecan be sewn; the artificial soft tissue transplant material does not need to be collected from patients themselves, so that the operation time is shortened, the occurrence rate of complications is reduced, and pain is relieved to the greatest extent.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to an oral membrane used for gingival reconstruction and a preparation method thereof. Background technique [0002] Periodontal tissue is composed of hard tissue (such as alveolar bone supporting teeth) and soft tissue (such as gingiva). Soft tissue includes attached gingiva attached to alveolar bone and free gingiva not attached to hard tissue. The attached gingiva starts from the free gingival sulcus and extends to the membranous gingiva to connect with the alveolar mucosa. Because it and the free gingiva are both keratinized gingiva, they are collectively called keratinized gingiva. Adequate attached gingiva is important for maintaining gingival health around natural teeth or implants and preventing loss of connective tissue. Patients with keratinized gingiva or insufficient attached gingiva, gingival recession, and root exposure affect the appearance; if the attac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/58A61L27/50C12P21/06C07K1/36C07K1/34C07K1/30
CPCA61L27/24A61L27/50A61L27/58A61L2430/34C07K14/78C12P21/06C08L89/00
Inventor 胡艳丽崔云宋天喜李洪景朱艳泽仇志烨
Owner 山东奥精生物科技有限公司
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