Protein feed derived from rhodopseudomonas palustris and preparation method and application of protein feed
A technology of swamp rhodopseudomonas and protein feed, which is applied in the field of animal husbandry feed, can solve the problems of unbalanced amino acid ratio, low protein utilization rate, expensive fish meal, etc., and achieves ideal ratio, high wall breaking efficiency, and animal growth performance. more effect
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Embodiment 1
[0039]The invention provides a protein feed derived from Rhodopseudomonas palustris, which consists of the following components in parts by weight: 91 parts of wall-breaking bacteria, 3 parts of lysine hydrochloride, 0.5 parts of methionine, threonine 0.5 parts, 1 part of arginine, 3 parts of calcium hydrogen phosphate and 1 part of calcium carbonate, a total of 100 parts, wherein the broken cell is Rhodopseudomonas palustris.
[0040] The preparation method of this protein feed comprises the following steps:
[0041] (1) Bacteria collection: Centrifuge the commercially available Rhodopseudomonas palustris bacteria liquid with a tubular centrifuge at a speed of 16000r / min for 10min, discard the supernatant, and collect the wet thallus. After determination, the moisture content is 25.5 %.
[0042] (2) Lysozyme pretreatment: Take 1000g of wet bacteria obtained in step (1), add 20mg of lysozyme, stir for 15min, then evenly spread it on a tray with a thickness of 0.2cm, and then ...
Embodiment 2
[0046] The invention provides a protein feed derived from Rhodopseudomonas palustris, which is composed of the following components in parts by weight: 92 parts of wall-breaking bacteria, 2.8 parts of lysine hydrochloride, 0.4 parts of methionine, threonine 0.4 parts, 0.4 parts of arginine, 2 parts of calcium hydrogen phosphate and 2 parts of calcium carbonate, a total of 100 parts, wherein the broken cell is Rhodopseudomonas palustris.
[0047] The preparation method of this protein feed comprises the following steps:
[0048] (1) Bacteria collection: Centrifuge the commercially available Rhodopseudomonas palustris bacteria liquid with a tubular centrifuge at a speed of 16000r / min for 10min, discard the supernatant, and collect the wet thallus. After determination, the moisture content is 26.2 %.
[0049] (2) Lysozyme pretreatment: Take 1000g of the wet bacteria obtained in step (1), add 5mg of lysozyme, stir for 15min, then evenly spread it on a tray with a thickness of 0.2...
Embodiment 3
[0053] The invention provides a protein feed derived from Rhodopseudomonas palustris, which is composed of the following components in parts by weight: 90 parts of wall-breaking bacteria, 3 parts of lysine hydrochloride, 0.5 parts of methionine, threonine 0.5 parts, 1 part of arginine, 3 parts of calcium hydrogen phosphate, and 2 parts of calcium carbonate, a total of 100 parts, wherein the broken cell is Rhodopseudomonas palustris.
[0054] The preparation method of this protein feed comprises the following steps:
[0055] (1) Bacteria collection: Centrifuge the commercially available Rhodopseudomonas palustris bacterium liquid with a tubular centrifuge at a speed of 16000r / min for 10min, discard the supernatant, and collect the wet thallus. After determination, the moisture content is 25.9 %.
[0056] (2) Lysozyme pretreatment: Take 1000g of wet bacteria obtained in step (1), add 1mg of lysozyme, stir for 15min, then evenly spread it on a tray with a thickness of 0.2cm, and...
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