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Protein feed derived from rhodopseudomonas palustris and preparation method and application of protein feed

A technology of swamp rhodopseudomonas and protein feed, which is applied in the field of animal husbandry feed, can solve the problems of unbalanced amino acid ratio, low protein utilization rate, expensive fish meal, etc., and achieves ideal ratio, high wall breaking efficiency, and animal growth performance. more effect

Inactive Publication Date: 2019-10-29
广州邹鲁农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a protein feed derived from Rhodopseudomonas palustris and its preparation method and application. The cell wall is broken, and the obtained cell wall is combined with nutrients such as lysine, methionine, threonine, arginine, calcium hydrogen phosphate, calcium carbonate, etc. to obtain protein feed derived from Rhodopseudomonas palustris. It can be efficiently absorbed and utilized by animals, which solves the problem that in the prior art, Rhodopseudomonas palustris has not been used to prepare protein feed and the protein utilization rate in protein feed is low, the price of fish meal contained in it is expensive, and Rhodopseudomonas palustris is only used to prepare protein feed The problem of unbalanced amino acid ratio

Method used

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Effect test

Embodiment 1

[0039]The invention provides a protein feed derived from Rhodopseudomonas palustris, which consists of the following components in parts by weight: 91 parts of wall-breaking bacteria, 3 parts of lysine hydrochloride, 0.5 parts of methionine, threonine 0.5 parts, 1 part of arginine, 3 parts of calcium hydrogen phosphate and 1 part of calcium carbonate, a total of 100 parts, wherein the broken cell is Rhodopseudomonas palustris.

[0040] The preparation method of this protein feed comprises the following steps:

[0041] (1) Bacteria collection: Centrifuge the commercially available Rhodopseudomonas palustris bacteria liquid with a tubular centrifuge at a speed of 16000r / min for 10min, discard the supernatant, and collect the wet thallus. After determination, the moisture content is 25.5 %.

[0042] (2) Lysozyme pretreatment: Take 1000g of wet bacteria obtained in step (1), add 20mg of lysozyme, stir for 15min, then evenly spread it on a tray with a thickness of 0.2cm, and then ...

Embodiment 2

[0046] The invention provides a protein feed derived from Rhodopseudomonas palustris, which is composed of the following components in parts by weight: 92 parts of wall-breaking bacteria, 2.8 parts of lysine hydrochloride, 0.4 parts of methionine, threonine 0.4 parts, 0.4 parts of arginine, 2 parts of calcium hydrogen phosphate and 2 parts of calcium carbonate, a total of 100 parts, wherein the broken cell is Rhodopseudomonas palustris.

[0047] The preparation method of this protein feed comprises the following steps:

[0048] (1) Bacteria collection: Centrifuge the commercially available Rhodopseudomonas palustris bacteria liquid with a tubular centrifuge at a speed of 16000r / min for 10min, discard the supernatant, and collect the wet thallus. After determination, the moisture content is 26.2 %.

[0049] (2) Lysozyme pretreatment: Take 1000g of the wet bacteria obtained in step (1), add 5mg of lysozyme, stir for 15min, then evenly spread it on a tray with a thickness of 0.2...

Embodiment 3

[0053] The invention provides a protein feed derived from Rhodopseudomonas palustris, which is composed of the following components in parts by weight: 90 parts of wall-breaking bacteria, 3 parts of lysine hydrochloride, 0.5 parts of methionine, threonine 0.5 parts, 1 part of arginine, 3 parts of calcium hydrogen phosphate, and 2 parts of calcium carbonate, a total of 100 parts, wherein the broken cell is Rhodopseudomonas palustris.

[0054] The preparation method of this protein feed comprises the following steps:

[0055] (1) Bacteria collection: Centrifuge the commercially available Rhodopseudomonas palustris bacterium liquid with a tubular centrifuge at a speed of 16000r / min for 10min, discard the supernatant, and collect the wet thallus. After determination, the moisture content is 25.9 %.

[0056] (2) Lysozyme pretreatment: Take 1000g of wet bacteria obtained in step (1), add 1mg of lysozyme, stir for 15min, then evenly spread it on a tray with a thickness of 0.2cm, and...

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Abstract

The invention provides protein feed derived from rhodopseudomonas palustris and a preparation method and application of the protein feed. The protein feed is prepared from the following components inparts by weight: 90-93 parts of a broken wall thallus, 2-3 parts of lysine hydrochloride, 0.1-0.5 part of methionine, 0.1-0.5 part of threonine, 0.3-1 part of arginine, 2-3 parts of calcium hydrophosphate and 1-2 parts of calcium carbonate, wherein the broken wall thallus is the rhodopseudomonas palustris; and the preparation method includes the steps of thallus collection, lysozyme pretreatment,thallus wall breaking and formula combination, the wall breaking rate of a rhodopseudomonas palustris thallus reaches 100% by adopting the preparation method, the amino acid ratio is ideal, and the animal absorption use ratio is high. The invention further discloses application of the protein feed derived from the rhodopseudomonas palustris in duroc*(large white*landrace) ternary miscellaneous piglets, Qingyuan local pullets, carassius auratus gibelio and South American white shrimps. The protein feed is used for replacing corresponding fish meal, animal growth performance is better or not affected, and the protein feed is ideal.

Description

technical field [0001] The invention relates to the technical field of animal husbandry feed, in particular to a protein feed derived from Rhodopseudomonas palustris and its preparation method and application. Background technique [0002] Protein is the material basis for the healthy growth of animals. The protein in feed mainly comes from animal protein (such as fish meal, meat and bone meal, plasma meal, etc.) and vegetable protein (such as soybean, rapeseed, cotton seed, etc.), and there is also a small amount of microbial protein ( Such as yeast powder in breweries); animal protein has high bioavailability, but is generally expensive and limited resources, such as fish meal, which is completely dependent on marine fishing, while plant protein generally has low bioavailability and anti-nutritional factors, such as beans There are anti-nutritional factors such as tannin, phytic acid and protease inhibitors in protein, which are not good for animal growth. [0003] Microb...

Claims

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Application Information

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IPC IPC(8): A23K10/16A23K20/142A23K20/24A23K20/26A23K50/75A23K50/30A23K50/60A23K50/80A23K40/00
CPCA23K10/16A23K20/142A23K20/24A23K20/26A23K40/00A23K50/30A23K50/60A23K50/75A23K50/80
Inventor 邹书通仲伟静
Owner 广州邹鲁农业有限公司
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