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Three-segment functional degradable artificial ligament regeneration scaffold and preparation method thereof

An artificial ligament and three-segment technology, applied in the field of three-segment functional degradable artificial ligament and its preparation, can solve the problems of easy failure, inability to induce ligament regeneration, inability to achieve physiological tendon-bone healing, etc., and achieve effective Preparation technology idea, simple preparation technology idea, good histocompatibility and effect of biomechanical properties

Active Publication Date: 2019-10-29
SHANGHAI SIXTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on the above, we conclude that the main problems faced by ligament regeneration are: there are defects such as inability to induce ligament regeneration, failure to achieve physiological tendon-bone healing, and easy failure.
However, so far, there have been no reports on the preparation of ligament repair scaffolds using electrospinning technology, braiding technology, and brittle fracture-casting-mold forming-freeze-drying technology.

Method used

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  • Three-segment functional degradable artificial ligament regeneration scaffold and preparation method thereof
  • Three-segment functional degradable artificial ligament regeneration scaffold and preparation method thereof

Examples

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Effect test

preparation example Construction

[0035] see figure 1 As shown, the present invention provides a method for preparing a three-stage functional degradable artificial ligament regeneration scaffold, which specifically includes the following steps:

[0036] Step 1: Dissolving or hot-melting the organic polymer material and silk fibroin in a mass ratio of 1:1 to 4:1 to obtain a composite spinning solution or a mixed drawing melt, which is ready for use;

[0037] Step 2: heat-melt silk fibroin, mesoporous nanoparticles loaded with tendon-bone healing growth factor, and tendon-bone healing growth factor at a mass ratio of 75:20:5 to 95:4:1 Mix and prepare a uniformly mixed casting solution with a certain concentration;

[0038] Step 3: Spinning or melt-drawing the composite spinning solution or molten drawing melt prepared in step 1) respectively to obtain nanofiber yarns or long monofilaments with different diameters and lengths;

[0039] Step 4: braiding the nanofiber yarn or long monofilament prepared in step 3...

Embodiment 1

[0057] refer to figure 1 As shown in the preparation process, this embodiment provides a method for preparing a three-stage functional degradable artificial ligament regeneration scaffold, which specifically includes the following steps:

[0058] Step 1: Weigh poly(glycolic acid) (PGA) / tussah silk fibroin (SF) with a mass ratio of 2:1 on an analytical balance, dissolve it in hexafluoroisopropanol at 25°C, and stir to dissolve until A clear homogeneous solution with a mass volume ratio (g / mL) of 15% was obtained;

[0059] Step 2: Dissolve the three components of silk fibroin, mesoporous nanoparticles loaded with tendon-bone healing growth factor, and tendon-bone healing growth factor according to the mass ratio of 75:20:5, and prepare a mass volume concentration of 20 % of casting liquid;

[0060] Step 3: The mixed solution spinning solution is then subjected to trumpet-conjugated electrospinning to obtain a PGA / SF composite nanofiber yarn with a diameter of 300 μm;

[0061]...

Embodiment 2

[0068] see continue reading figure 1 As shown in the preparation process, this embodiment provides a method for preparing a three-stage functional degradable artificial ligament regeneration scaffold, which specifically includes the following steps:

[0069] Step 1: Weigh poly-L-lactic acid (PLLA) / tussah silk fibroin (SF) with a mass ratio of 3:1 on an analytical balance, heat and stir at 90°C to obtain a mixed drawing melt, and set aside;

[0070] Step 2: Dissolve the three components of silk fibroin, mesoporous nanoparticles loaded with tendon-bone healing growth factor, and tendon-bone healing growth factor according to the mass ratio of 80:16:4, and prepare a mass volume concentration of 20 % of casting liquid;

[0071] Step 3: Melt and draw the molten wire drawing solution to obtain a PLLA / SF composite long monofilament with a diameter of 150 μm;

[0072] Step 4: After pulling the PLLA / SF composite long monofilament, use 16 parallel threads, twisting and knitting techno...

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Abstract

The invention discloses a three-segment functional degradable artificial ligament regeneration scaffold and a preparation method thereof. The preparation method comprises the steps: a composite spinning solution or a mixed drawing molten solution is prepared; a casting solution is prepared; nanofiber yarn or long monofilaments are prepared; a tubular textile is woven; an assembled mold sleeves thetubular textile for heat setting; a first-segment complex with a cylindrical mold body is prepared; a first-segment and second-segment complex with the cylindrical mold body are prepared; then the casting solution continues being cast into the cylindrical mold bodies, and thus a three-segment scaffold complex is obtained; and finally, the three-segment scaffold complex is subjected to electrostatic spinning, and then the three-segment functional degradable artificial ligament regeneration scaffold is obtained. The three-segment functional degradable artificial ligament regeneration scaffold prepared through the preparation method can structurally perform functions correspondingly, has the great biomechanical property and biocompatibility, and can be used for regeneration treatment of diseases such as rotator cuff, tendon and ligament injuries; and the preparation method is simple, efficient and low in price, and has great application prospects.

Description

technical field [0001] The invention relates to the technical field of a three-stage functional degradable artificial ligament and its preparation method, in particular to a mechanically enhanced ligament regeneration scaffold for rapidly promoting tendon-bone and preventing postoperative loosening and its preparation method. Background technique [0002] Effective repair of the anterior cruciate ligament (ACL) after injury is currently a major clinical problem, mainly due to the weak self-healing ability of the completely ruptured ACL, and the high rate of postoperative loosening and re-rupture. There are two main reasons for the failure of ACL reconstruction. One is that the intrinsic characteristics of the graft used for reconstruction cannot effectively match the autologous ligament tissue. For example, allogeneic tendon, autologous tendon, and artificial ligament are all alternatives to the damaged ligament. , but there is the rejection of the allograft tendon and the s...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/18A61L27/10A61L27/12A61L27/50A61L27/54A61L27/58
CPCA61L27/227A61L27/18A61L27/10A61L27/12A61L27/50A61L27/54A61L27/58A61L2430/10A61L2300/414A61L2400/12A61L2300/412A61L2300/602C08L89/00C08L67/04
Inventor 赵金忠朱同贺蒋佳燕晓宇康育豪王立人徐俊杰李亚民
Owner SHANGHAI SIXTH PEOPLES HOSPITAL
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