Grifola frondosa strain obtained by ultraviolet mutagenesis

Abstract

The invention discloses a grifola frondosa strain obtained by ultraviolet mutagenesis, and relates to the field of food microbial technology application. The mutagenized strain is already deposited atthe China Center for Type Culture Collection (CCTCC) in Wuhan on December 19, 2018, and numbered as CCTCC M 2018907, the proposed classified name is grifola frondosa strain JSULIUWANG18, and the Latin name is Grifola frondosa JSULIUWANG18. The new strain of grifola frondosa is used for liquid fermentation of a culture medium using bran and rice bran complete feed as the main components and meeting the standards of limited use of pollutants after inspection, and after a fermented material of a grifola frondosa mycelium raw material and fermentation liquid is produced, the fermented material issubjected to multi-frequency ultrasonic-assisted extraction, freeze concentration, replacement of heavy metal ions by citric acid extraction, alcohol precipitation and ethanol washing and lyophilization to obtain qualified grifola frondosa polysaccharide products.

Description

technical field [0001] The present invention relates to the application field of food microbiology technology, in particular to a new strain of Grifola frondosa obtained through ultraviolet mutagenesis, which is used for liquid fermentation in a culture medium whose main components are bran and rice bran which have reached the pollutant limit use standard. , after producing the raw material of Grifola frondosa mycelium and the fermented product of the fermentation liquid, the qualified Grifola frondosa polysaccharide is obtained through multi-frequency ultrasonic-assisted extraction, freeze concentration, citric acid extraction to replace heavy metal ions, alcohol precipitation and alcohol washing, and freeze-drying product. Background technique [0002] Many medicinal and edible fungi or medicinal fungi have a long history of use in China and a wide range of users. Modern science reveals that the mycelia, fruiting bodies or spores of commonly used rare fungi such as Grifol...

AI Technical Summary

Problems solved by technology

In the past, we always hoped that the results of our research could deal with various bran and rice bran, so we intentionally relaxed the restrictions on the use of bran and rice bran raw materials, and did not formulate raw material standards. Some outliers in the research range, such as the problem of exceeding the standard of a certain heavy metal that was not measured in the previous research, are due to the complexity of the raw materials of bran and rice bran, the quality is difficult to maintain a similar level, and unknown pollutants eventually enter the final product

Images