Grifola frondosa strain obtained by ultraviolet mutagenesis

A technology of Grifola frondosa and strains, applied in the field of a Grifola frondosa strain obtained through ultraviolet mutagenesis, can solve the problems of excessive heavy metal determination results, difficulty in maintaining a similar level of quality, complicated sources of bran and rice bran raw materials, etc., to achieve The effect of simple and easy technology

Pending Publication Date: 2019-11-01
JIANGSU UNIV
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Problems solved by technology

In the past, we always hoped that the results of our research could deal with various bran and rice bran, so we intentionally relaxed the restrictions on the use of bran and rice bran raw materials, and did not formulate raw material standards. Some outliers in the research range, such as

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  • Grifola frondosa strain obtained by ultraviolet mutagenesis
  • Grifola frondosa strain obtained by ultraviolet mutagenesis
  • Grifola frondosa strain obtained by ultraviolet mutagenesis

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[0064] Example 1

[0065] The Grifola frondosa strain adopts the new Grifola frondosa strain obtained by the present invention, with the preservation number CCTCC NO: M 2018907. The amount of rice bran used is 5.0g / 100mL medium, the amount of bran used is 5.0g / 100mL medium, add potassium dihydrogen phosphate 0.25g / 100mL, magnesium sulfate heptahydrate 0.15g / 100mL, pH is natural, add water to the required It was sterilized at 121°C for 30min, used as a culture medium for fermentation, the sample volume in the shake flask was 40%, the inoculum volume was 10%, the culture temperature was 23°C, the rotation speed was 150r / min, and the culture time was 8d. The following steps (1) ~ (5) are implemented: (1) the mycelium containing a small amount of rice bran obtained by liquid culture is separated with a 60-mesh metal screen and washed twice with water; (2) the obtained Wet mycelium is extracted with multi-frequency ultrasonic assisted warm water, the water temperature is not more tha...

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Abstract

The invention discloses a grifola frondosa strain obtained by ultraviolet mutagenesis, and relates to the field of food microbial technology application. The mutagenized strain is already deposited atthe China Center for Type Culture Collection (CCTCC) in Wuhan on December 19, 2018, and numbered as CCTCC M 2018907, the proposed classified name is grifola frondosa strain JSULIUWANG18, and the Latin name is Grifola frondosa JSULIUWANG18. The new strain of grifola frondosa is used for liquid fermentation of a culture medium using bran and rice bran complete feed as the main components and meeting the standards of limited use of pollutants after inspection, and after a fermented material of a grifola frondosa mycelium raw material and fermentation liquid is produced, the fermented material issubjected to multi-frequency ultrasonic-assisted extraction, freeze concentration, replacement of heavy metal ions by citric acid extraction, alcohol precipitation and ethanol washing and lyophilization to obtain qualified grifola frondosa polysaccharide products.

Description

technical field [0001] The present invention relates to the application field of food microbiology technology, in particular to a new strain of Grifola frondosa obtained through ultraviolet mutagenesis, which is used for liquid fermentation in a culture medium whose main components are bran and rice bran which have reached the pollutant limit use standard. , after producing the raw material of Grifola frondosa mycelium and the fermented product of the fermentation liquid, the qualified Grifola frondosa polysaccharide is obtained through multi-frequency ultrasonic-assisted extraction, freeze concentration, citric acid extraction to replace heavy metal ions, alcohol precipitation and alcohol washing, and freeze-drying product. Background technique [0002] Many medicinal and edible fungi or medicinal fungi have a long history of use in China and a wide range of users. Modern science reveals that the mycelia, fruiting bodies or spores of commonly used rare fungi such as Grifol...

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Application Information

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IPC IPC(8): C12N1/14A01H15/00C12N13/00C12R1/645
CPCA01H15/00C12N13/00C12R2001/645C12N1/145
Inventor 刘伟民汪涛杨卫卫余昭玮沈国栋任晓锋程宇何荣海马海乐
Owner JIANGSU UNIV
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