Method for extracting mildew intracellular polysaccharide by using green solvent
A green solvent and intracellular polysaccharide technology, applied in the field of active substance extraction, can solve the problems of low purity, low extraction efficiency, and environmental pollution of polysaccharides
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Embodiment 1
[0033] The embodiment of the present application provides the first green solvent extraction experiment of polysaccharides from waste industrial Aspergillus niger mycelium, and the specific steps are as follows:
[0034] 1. Preparation of green solvent: Mix betaine and 1,2-propanediol at a molar ratio of 1:4, stir and mix at 80°C and 150 rpm for 2 hours, stop heating when the mixture becomes clear, and cool to room temperature.
[0035] 2. Extracting polysaccharides: Accurately weigh 5 g of an aqueous solution (50% w / w) of the green solvent of step 1 in a flask. Add 200 mg of waste industrial Aspergillus niger mycelium (waste mycelium after mechanically breaking the wall and extracting fermentation products after fermentation for 72 hours), add a stir bar and place it on a magnetic stirrer. Under the conditions of temperature 50° C. and 150 rpm, extract for 2 hours. Then cool to room temperature, centrifuge the mixed solution, and take the supernatant; add ethanol to the supe...
Embodiment 2
[0040] The embodiment of the present application provides the second experiment of extracting polysaccharides from waste industrial Aspergillus niger mycelium with green solvent, and the specific steps are as follows:
[0041] 1. Preparation of green solvent: Mix betaine and lactic acid at a molar ratio of 1:2, stir and mix at 80°C, 150rpm for 2 hours, stop heating when the mixture becomes clear, and cool to room temperature.
[0042] 2. Extracting polysaccharides: Accurately weigh 5 g of an aqueous solution (20% w / w) of the green solvent in step 1, and add it into a flask. Add 200 mg of waste industrial Aspergillus niger mycelia (mycelia after mechanically breaking the wall and extracting fermentation products after fermentation for 72 hours), add a stirring bar and place it on a magnetic stirrer. Extraction was carried out for 3 hours at a temperature of 35° C. and 150 rpm. Then cool to room temperature, centrifuge the mixed solution, take the supernatant, add ethanol to th...
Embodiment 3
[0047] The embodiment of the present application provides the third experiment of extracting polysaccharides from waste industrial Aspergillus niger mycelium with green solvent, and the specific steps are as follows:
[0048] 1. Preparation of green solvent: Mix choline chloride: lactic acid in a molar ratio of 1:1, stir and mix at 80°C and 150rpm for 2 hours, stop heating when the mixture becomes clear, and cool to room temperature.
[0049] 2. Extracting polysaccharides: Accurately weigh 5 g of an aqueous solution (10% w / w) of the green solvent in step 1, and add it to the flask. Add 200 mg of waste industrial Aspergillus niger mycelia (mycelia after mechanically breaking the wall and extracting fermentation products after fermentation for 72 hours), add a stirring bar and place it on a magnetic stirrer. Under the conditions of temperature 40° C. and 150 rpm, extract for 1.5 hours. Then cool to room temperature, centrifuge the mixed solution, take the supernatant, add ethan...
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