Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for preparing natural collagen/nano gold compound and synchronously monitoring natural collagen self-assembly

A collagen and self-assembly technology, applied in the fields of nano-drugs, nano-technology, nano-technology, etc., can solve the problems of complex instruments and equipment, cumbersome sample preparation, high testing cost, etc., and achieve the effect of simple operation and low price.

Active Publication Date: 2019-11-08
WUHAN POLYTECHNIC UNIVERSITY
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although electron microscopy has achieved satisfactory results in obtaining microscopic information on collagen self-assembly, this technique requires complex instruments and equipment, and has defects such as cumbersome sample preparation and high testing costs.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing natural collagen/nano gold compound and synchronously monitoring natural collagen self-assembly
  • Method for preparing natural collagen/nano gold compound and synchronously monitoring natural collagen self-assembly
  • Method for preparing natural collagen/nano gold compound and synchronously monitoring natural collagen self-assembly

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Weigh an appropriate amount of bovine Achilles tendon natural collagen sample, prepare 10 mL of a sample with a concentration of 2 mg / mL with 0.5 mol / L acetic acid, add 1 mL of 0.002 mol / L chloroauric acid solution, stir for 1 hour, and slowly add 0.1 mol / L 0.6 mL of sodium borohydride solution in L and stirred for 12 hours. Then put the prepared natural collagen solution into a dialysis bag with a molecular weight cut-off of 12,000 Daltons, use a phosphate buffer solution with a pH of 8.0 as the dialysate, change the dialysate every 3 hours, and dialyze for 48 hours to obtain bovine Achilles tendon Collagen / gold nanocomposites are ready for use. Take 4 mL of the complex and incubate it in a 35 °C incubator for 3 hours, and measure the ultraviolet absorption spectrum of the complex before and after self-assembly by a UV spectrophotometer.

[0035] figure 1 The ultraviolet spectrograms of the natural collagen and the natural collagen / gold nanocomposite of Example 1 are...

Embodiment 2

[0039]Weigh an appropriate amount of bovine Achilles tendon collagen sample, prepare 10 mL of a sample with a concentration of 1 mg / mL with 0.5 mol / L acetic acid, add 1 mL of 0.001 mol / L chloroauric acid solution, stir for 1 hour, and slowly add 0.05 mol / L 0.6 mL of sodium borohydride solution and stirred for 12 hours. Then put the prepared collagen solution into a dialysis bag with a molecular weight cut-off of 12,000 Daltons, use a phosphate buffer solution with a pH of 8.0 as the dialysate, change the dialysate every 3 hours, and dialyze for 48 hours to obtain bovine Achilles tendon collagen The protein / nanogold complex is ready for use. Take 4 mL of the complex and incubate it in a 35 °C incubator for 3 hours, and measure the ultraviolet absorption spectrum of the complex before and after self-assembly by a UV spectrophotometer.

[0040] Figure 4 The ultraviolet spectrograms before and after the self-assembly of the natural collagen / gold nanocomposite in Example 2 are s...

Embodiment 3

[0042] Weigh an appropriate amount of grass carp skin collagen sample, prepare 10 mL of a sample with a concentration of 2 mg / mL with 0.5 mol / L acetic acid, add 1 mL of 0.001 mol / L chloroauric acid solution, stir for 1 hour, and slowly add 0.05 mol / L of Sodium borohydride solution 0.6mL, and stirred for 12 hours. Then put the prepared collagen solution into a dialysis bag with a molecular weight cut-off of 12,000 Daltons, use a phosphate buffer solution with a pH of 8.0 as the dialysate, change the dialysate every 3 hours, and dialyze for 48 hours to obtain bovine Achilles tendon collagen The protein / nanogold complex is ready for use. Take 4 mL of the complex and incubate it in a 30°C incubator for 3 hours, and measure the ultraviolet absorption spectrum of the complex before and after self-assembly by a UV spectrophotometer.

[0043] Figure 5 The ultraviolet spectrograms before and after the self-assembly of the natural collagen / gold nanocomposite in Example 3 are shown. ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for preparing a natural collagen / nano gold compound and synchronously monitoring natural collagen self-assembly. The method comprises the steps: (1) dissolving naturalcollagen by using an acetic acid water solution to obtain a natural collagen solution; (2) evenly mixing the natural collagen solution with a chloroauric acid solution; (3) evenly mixing a reducing agent solution with the solution obtained in the step (2); (4) dialyzing the solution obtained in the step (3) to obtain the collagen / nano gold compound before self-assembly, determining the absorbanceof the collagen / nano gold compound under 515-525 nm, and recording it as A0; (5) self-assembling the solution obtained in the step (4), determining the absorbance of the self-assembled collagen / nanogold compound under 515-525 nm, and recording it as A1; (6) when the ratio of A1 to A0 is greater than 1, determining that the self-assembly is completed, and when the ratio of A1 to A0 is smaller than or equal to 1, continuing to carry out self-assembly. The monitoring method is simple to operate and low in cost, and can present multiple information of collagen self-assembly.

Description

technical field [0001] The invention belongs to the interdisciplinary field of biology, chemistry, materials and other technologies, and more specifically relates to a natural collagen / nano-gold compound and a method for synchronously monitoring the self-assembly of natural collagen. Background technique [0002] Collagen is one of the most abundant and widely distributed protein types in animal connective tissue, and its structural domain is characterized by a triple helix structure formed by three peptide chain helical windings. Due to its good biocompatibility, low immune activity and superior biodegradability, collagen is widely used in biomaterials, food science, medical cosmetology and other fields. Self-assembly is one of the most important molecular behaviors of collagen. In vivo, collagen with a triple helix structure can spontaneously assemble to form microfibrils, which are further bundled to form a fibrous network structure, and provide a platform for cell growt...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/31G01N21/33B22F9/24B82B3/00B82Y5/00B82Y30/00
CPCG01N21/31G01N21/33B22F9/24B82B3/0009B82Y30/00B82Y5/00B22F2009/245
Inventor 未本美汪海波钟华英康德来张军涛许承志
Owner WUHAN POLYTECHNIC UNIVERSITY
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com