Preparation method of arachidonic acid (ARA) emulsion
A technology of arachidonic acid and emulsion, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve the problems of ARA content and quality reduction, high temperature oxidation, etc., and achieve the effect of ensuring content and quality
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Embodiment 1
[0025] In a preferred embodiment of the present invention, the preparation method of the arachidonic acid emulsion comprises the steps of:
[0026] (1) Mortierella alpine fermentation;
[0027] The preparation method of fermentation medium comprises the steps:
[0028] To prepare enriched media:
[0029] Add glucose 25mg / L, yeast powder 10mg / L, FeCl to distilled water with a pH value of 7.0 2 15mg / L, Na 2 EDTA 3mg / L,H 3 BO 3 2mg / L, MnCl 2 ·4H 2 O 1mg / L, ZnSO 4 ·7H 2 O 0.2mg / L, CuSO 4 ·5H 2 O0.06mg / L, Na 2 MoO 4 ·2H 2 O 0.1mg / L, CoCl 2 ·6H 2 O 0.1mg / L, the enriched medium is obtained after heating the solution to 60°C;
[0030] Prepare Growth Medium No. 1
[0031] Take 0.8 mg / L of sodium glutamate and 0.8 mg / L of propionic acid, stir and mix, and heat the solution to 40°C to obtain the No. 1 growth medium;
[0032] Prepare Growth Medium No. 2
[0033] Take sodium nitrate 9.5mg / L, dipotassium hydrogen phosphate 4mg / L, magnesium chloride 1.5mg / L, sodium chlor...
Embodiment 2
[0040] The fermentation medium preparation method of Mortierella alpina fermentation in the present embodiment comprises the steps:
[0041] To prepare enriched media:
[0042] Add glucose 30mg / L, yeast powder 10mg / L, FeCl to distilled water with a pH value of 7.0 2 5mg / L, Na 2 EDTA 2mg / L,H 3 BO 3 1mg / L, MnCl 2 ·4H 2 O 2mg / L, ZnSO 4 ·7H 2 O 0.5mg / L, CuSO 4 ·5H 2 O0.15mg / L, Na 2 MoO 4 ·2H 2 O 0.2mg / L, CoCl 2 ·6H 2 O 0.2mg / L, the enrichment medium is obtained after heating the solution to 50°C;
[0043] Prepare Growth Medium No. 1
[0044] Take sodium glutamate 1.0mg / L, propionic acid 0.8mg / L, stir and mix, and heat the solution to 40°C to obtain the No. 1 growth medium;
[0045] Prepare Growth Medium No. 2
[0046]Take 10 mg / L of sodium nitrate, 6 mg / L of dipotassium hydrogen phosphate, 2 mg / L of magnesium chloride, 20 mg / L of sodium chloride, and 1.1 mg / L of yeast paste, stir and mix, and heat the solution to 60°C to obtain the 2 No. growth medium;
[0047...
Embodiment 3
[0051] The fermentation medium preparation method of Mortierella alpina fermentation in the present embodiment comprises the steps:
[0052] To prepare enriched media:
[0053] To distilled water with pH value of 7.0, add glucose 35mg / L, yeast powder 15mg / L, FeCl 2 3mg / L, Na 2 EDTA 6mg / L, H 3 BO 3 3mg / L, MnCl 2 ·4H 2 O 2mg / L, ZnSO 4 ·7H 2 O 0.4mg / L, CuSO 4 ·5H 2 O0.1mg / L, Na 2 MoO 4 ·2H 2 O 0.3mg / L, CoCl 2 ·6H 2 0.3 mg / L, the enriched medium was obtained after the solution was heated to 60°C;
[0054] Prepare Growth Medium No. 1
[0055] Take 1.2 mg / L of sodium glutamate and 0.8 mg / L of propionic acid, stir and mix, and heat the solution to 30°C to obtain the No. 1 growth medium;
[0056] Prepare Growth Medium No. 2
[0057] Take 10.5 mg / L of sodium nitrate, 5 mg / L of dipotassium hydrogen phosphate, 2.5 mg / L of magnesium chloride, 21 mg / L of sodium chloride, and 1.2 mg / L of yeast extract, stir and mix, and heat the solution to 60 ℃ to obtain the The above-m...
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