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Biosensor based on double-signal amplification and heavy metal lead ion detection

A biosensor, dual-signal amplification technology, applied in biochemical equipment and methods, microbial determination/inspection, instruments, etc., can solve the problems of destroying DNAzyme catalytic activity, limiting detection sensitivity, etc., to promote development and improve immobilization capacity. , the effect of the simple method

Active Publication Date: 2019-11-12
CHONGQING MEDICAL UNIVERSITY
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally, some strategies immobilize 8-17DNAzyme on the electrode surface or modify it with fluorescent groups or redox active groups to detect Pb 2+ , which may limit detection sensitivity or disrupt the catalytic activity of the DNAzyme

Method used

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  • Biosensor based on double-signal amplification and heavy metal lead ion detection
  • Biosensor based on double-signal amplification and heavy metal lead ion detection
  • Biosensor based on double-signal amplification and heavy metal lead ion detection

Examples

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Embodiment 1

[0047] Step 1. Prior to sensor construction, 2 μM 5′-SH-labeled capture probe H 1 Heat at 95°C for 5 minutes. Then, the solution was slowly cooled to room temperature to form a stem-loop structure;

[0048] Step 2. Use 0.3 and 0.05 μm Al respectively 2 o 3 Polish the electrode into a mirror surface with powder, then ultrasonically ultrasonicate the electrode for 5 minutes each in the order of ultrapure water, absolute ethanol, and ultrapure water, and dry at room temperature for later use;

[0049] Step 3. Deposit the dried electrode with 1% HAuCl4 solution for 30s under constant voltage (-0.2V), finally form a uniform AuNPs layer, and wash it with ultrapure water;

[0050] Step 4. The cleaned and dried electrode was sealed with 0.5mM MCH for 30 minutes and rinsed with ultrapure water;

[0051] Step 5. After drying, drop 10 μL of 2 μM hairpin structure capture probe H on the electrode surface 1 , and incubated at 37°C for 1 hour;

[0052] Step 6. After rinsing and drying...

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Abstract

The invention relates to a preparation method and application of an electrochemical sensor for heavy metal lead ion detection and belongs to the technical field of electrochemical detection. The biosensor is characterized in that firstly, an NG-PTCA nanomaterial is synthesized, then, HAuCl4 is reduced to gold nanoparticles (AuNPs) through Thi and modified on the NG-PTCA nanomaterial to obtain an NG-PTCA-Thi-Au nanocomposite, and a hairpin-like DNA signal probe is mixed with the composite to prepare a biological signal probe; before construction of the sensor, 8-17 DNAzyme is cut in vitro in the presence of Pb<2+>; then, the hairpin-like capture probe is fixed by a gold deposition electrode; after introduction of a cut fragment S1, a catalytic hairpin assembly process is started and the biological signal probe is fixed, so that double-signal detection of heavy metal lead ions is realized. The sensor is successfully applied to detection of lead ions in the environment. The biosensor hasthe advantages of high sensitivity, high specificity and convenience in detection. The biosensor provides experiment basis for research and development of the lead ion detection technology and provides a new thought and a new technical platform for monitoring of lead ions in the environment.

Description

Technical field: [0001] The invention relates to a preparation method and application of an electrochemical sensor for quantitatively detecting heavy metal lead ions in the environment, especially perylene-3,4,9,10-tetrafluoroethylene based on catalytic hairpin assembly and modification of thionine and gold nanoparticles Formic acid-dispersed nitrogen-doped graphene is used as a signal probe to prepare a dual-signal amplification biosensor, and the specific catalytic function of 8-17 DNAzyme is used to detect heavy metal lead ions, which belongs to the field of electrochemical detection. Background technique: [0002] Lead ion (Pb 2+ ) is considered a heavy metal pollutant that poses a threat to the sustainability of humans and ecosystems. It can accumulate in the body and cause various diseases, including neurological, reproductive, cardiovascular and other diseases. Even with low doses of Pb 2+ It is also highly toxic, affecting the growth and intellectual development o...

Claims

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Application Information

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IPC IPC(8): C12Q1/682C12Q1/6825C12Q1/44G01N27/327G01N27/48
CPCC12Q1/682C12Q1/6825C12Q1/44G01N27/3276G01N27/3277G01N27/3278G01N27/48C12Q2563/137C12Q2525/301C12Q2521/345C12Q2565/607Y02A20/20
Inventor 何俊琳于超马一丹
Owner CHONGQING MEDICAL UNIVERSITY
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