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Microorganism curing kit and calcareous sand in-site curing method

An in-situ solidification, microbial technology, applied in the field of geological engineering-microbiology interdisciplinary, can solve the problems of high cost, long construction period, and great environmental damage, and achieve the effect of saving cost and increasing pore channels.

Active Publication Date: 2019-11-12
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional foundation reinforcement method is to use mechanical energy or artificial materials to physically or chemically reinforce the soil. Physical reinforcement methods include measures such as dynamic compaction of the foundation, replacement of soil cushions, and installation of cement mixing piles. The construction period is long, the cost is high, and the site is required. Supports the deployment of large machinery and equipment, in many cases the application is limited
The chemical reinforcement method is to pour chemical grout into the target rock and soil mass, but because most of the grouting materials are strongly alkaline and biologically toxic, the solidified rock and soil mass cannot grow vegetation, which is too harmful to the environment

Method used

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  • Microorganism curing kit and calcareous sand in-site curing method
  • Microorganism curing kit and calcareous sand in-site curing method
  • Microorganism curing kit and calcareous sand in-site curing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] The object of solidification in this embodiment is calcareous sand in the South China Sea with a calcium content of 95.13%, and the particle gradation is shown in Table 2.

[0065] Calcareous sand gradation used in the embodiment 1 of table 2

[0066]

[0067] A method for microbial in-situ solidification of calcareous sand, which utilizes microbial-induced mineralization technology to target coarse sand with a particle size greater than 0.50mm and a particle content of less than 25% of the total weight. Sand, or medium sand with a particle size greater than 0.25mm in the content of more than 50% of the total weight and a particle size greater than 0.50mm in the content of less than 50% of the total weight, the mass fraction of the calcareous sand body 2 is sprayed sequentially to 20% The acetic acid solution, microbial bacteria solution and solidification solution composed of urea and nutrient solution solidify the calcareous sand body 2 to form a cemented block 1. ...

Embodiment 2

[0074] The object of solidification in this embodiment is calcareous sand in the South China Sea with a calcium content of 95.13%, and the particle gradation is shown in Table 3.

[0075] Calcareous sand gradation used in the embodiment 2 of table 3

[0076]

[0077] Respectively for the fine sand whose particle size is larger than 0.075mm and the particle content is more than 85% of the total weight, and the particle size is larger than 0.25mm. For silt with a weight of 85%, grouting holes are evenly opened on the sand body to be treated. The inner diameter of the grouting hole is 0.1m, the hole depth is 0.2m, and the interval between adjacent grouting holes is 1.0m, and the square grid is evenly arranged. The method of in-situ solidification of calcareous sand is as follows:

[0078] 1) if image 3 As shown, a sufficient amount of calcareous sand was collected on the spot near the target area, poured into a stirring container containing 20% ​​acetic acid solution by mas...

Embodiment 3

[0084] This embodiment is aimed at large-scale construction projects with high requirements for foundation bearing capacity and stability. The curing object is calcareous sand from the South China Sea with a calcium content of 95.13%, and the particle gradation is medium sand. See Table 2. Spray and inject In the grout combination method, grouting holes are opened on the calcareous sand body to be treated. The inner diameter of the grouting hole is 0.2m, the hole depth is 1.0m, and the interval between adjacent grouting holes is 1.5m, and the square grid is evenly arranged.

[0085] 1) if figure 2 As shown, at 25°C, spray the acetic acid solution with a mass fraction of 25% on the surface of the calcareous sand in the target area, and the spray volume is 2500kg / m 2 , keep for 12h;

[0086] 2) Use the water pump 10 to inject the liquid culture medium mixture of the activated Bacillus sarcina through the grouting pipe into the soil body along the grouting hole, and the injecti...

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Abstract

The invention discloses a microorganism curing kit and a calcareous sand in-site curing method, and belongs to the field of geological engineering and microorganism interdisciplines. The microorganismcuring kit is used for calcareous sand in-site curing and comprises a first component containing carboxylic acid, a second component containing microorganisms capable of generating urease, and a third component containing urea and a nutrient solution. The calcareous sand in-site curing method comprises the step that calcareous sand is partially dissolved by the first component. A calcium source can be efficiently obtained from sandy soil of an in-situ area or a nearby area of a calcareous sand soil body and then reacts with the microorganisms to deposit calcium carbonate, and the in-situ curing effect is realized.

Description

technical field [0001] The invention belongs to the interdisciplinary field of geological engineering and microbes, and more specifically relates to a microbial solidification kit and a method for solidifying calcareous sand in situ. Background technique [0002] Microbial mineralization exists widely in nature. Some microorganisms can use their own physiological activities or metabolism to induce the production of various minerals in vitro. These crystallization products will have a certain impact on the physical and mechanical properties of rock and soil. Widely concerned by the academic circles. Because calcite is relatively stable in nature, has good strength and durability, and can well stabilize sandy soil, microbially induced calcium carbonate precipitation (MICP) has always been a hot spot in the research of microbial geotechnical technology. The MICP method uses some specific urease-producing bacteria, such as Sporosarcina pasteurii, to hydrolyze urea in an alkalin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): E02D3/12C12N1/20C12R1/01
CPCE02D3/12C12N1/20
Inventor 唐朝生李昊尹黎阳吕超刘博谢约翰程青张宽施斌
Owner NANJING UNIV
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