Method for authenticating unsaturated lipid carbon-carbon double bond position based on epoxidation and mass spectrum

A carbon-carbon double bond, unsaturated fatty acid technology, applied in the field of mass spectrometry analysis, can solve problems such as the yield of side reactions, and achieve the effects of simple and easy method, high yield, and improved detection sensitivity and quantitative accuracy.
CN110441383AInactive Publication Date: 2019-11-12TSINGHUA UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TSINGHUA UNIV
Publication Date
2019-11-12
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention discloses a method for authenticating an unsaturated lipid carbon-carbon double bond position based on epoxidation and a mass spectrum. The method comprises: performing epoxidation derivation on to-be-analyzed unsaturated lipid by using low-temperature plasma; performing tandem mass spectrum analysis on an epoxidation product that is obtained after the epoxidation derivationon the unsaturated lipid, to obtain a specific ion at the carbon-carbon double bond position; and authenticating the unsaturated lipid carbon-carbon double bond position according to the specific ion.The method is simple and practicable without transforming the mass spectrum. The epoxidation derivation of the low-temperature plasma is combined with the tandem mass spectrum, so that the carbon-carbon double bond position in the unsaturated lipid can be fast and accurately authenticated.
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Description

technical field

[0001] The invention relates to the technical field of mass spectrometry analysis methods, in particular to a method for identifying the carbon-carbon double bond position of unsaturated lipids based on epoxidation and mass spectrometry. Background technique

[0002] Lipids have many important biological functions. Unsaturated lipids are an important part of cell membranes and participate in important biological processes such as energy storage and signal transduction. Studies have shown that the increase in the content of unsaturated fatty acids is closely related to the occurrence of various cancers. For example, studies have found that in ovarian cancer stem cells, increased lipid unsaturation is an important metabolic marker and can be used as a target for cancer therapy. The composition of lipid carbon-carbon double bond (C=C) isomers can also be used to distinguish normal tissue from cancerous diseased tissue. Therefore, in order to better understand ...

Claims

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