Cell circulation biological artificial liver device with oxygen supply

Abstract

The invention discloses a cell circulation biological artificial liver device with oxygen supply, relates to the technical field of medical equipment, and solves the technical problems of improving working efficiency and liver cell activity. The artificial liver device comprises a blood introducing pipe, a blood returning pipe, a hepatocyte pump, a membrane oxygenator, a cell culture device, two-stage plasma separators and two-stage blood pumps; after the blood in the body of a patient is led out by the blood introducing pipe, the blood sequentially passes through a primary blood pump, the primary side of a primary plasma separator and the blood returning pipe, and then returns to the body of the patient; the plasma separated from the secondary side of the primary plasma separator sequentially passes through a secondary blood pump, the primary side of a secondary plasma separator and the blood returning pipe, and then returns to the body of the patient; and after matrix carrying hepatocytes in the cell culture device flows out from a liquid outlet, the matrix sequentially passes through the secondary side of the secondary plasma separator, the hepatocyte pump and the membrane oxygenator, and then returns to the cell culture device. The artificial liver device provided by the invention can realize the functions of detoxification, metabolism and synthesis of the liver.

Description

technical field [0001] The invention relates to medical equipment technology, in particular to the technology of a cell circulation bioartificial liver device with oxygen supply. Background technique [0002] The artificial liver support system has brought opportunities and hope for the recovery of liver failure patients or waiting for liver transplantation, and it is an important research topic in the field of liver disease treatment. According to whether it contains biologically active liver cells, artificial liver support systems are mainly divided into two categories: non-biological artificial liver and biological artificial liver. [0003] The non-biological artificial liver mainly has the function of detoxification, and is composed of plasma separator, plasma adsorber, hemodialyzer and other components and the corresponding extracorporeal circulation device. Since the function of the liver is very complex, in addition to the detoxification function, it also includes f...

AI Technical Summary

Problems solved by technology

The biggest advantage of the hollow fiber tube reactor is that it has a large surface area / volume ratio, which is convenient for metabolite transport and keeps the minimum dead space. However, due to the uneven distribution of liver cells in the reactor, it is easy to cause a decrease in cell viability;

[0007] 2) Single-layer / multi-layer plate type: This reactor is to plant hepatocytes directly on the plate. The advantage is that the cells are evenly distributed and the microenvironment is consistent, but the ratio of its surface area to volume is relatively small, and the equipment area and dead space Also large, low work efficiency;

[0008] 3) Perfusion bed / stent type: The bioreactor provides a solid phase carrier to make the hepatocytes adhere and fix, and the hepatocytes adhere and grow in the pores, which greatly reduces the shear force damage caused by the flow of the medium, and the cells can maintain higher functions Vitality, its disadvantages are uneven perfusion, low work efficiency, and easy blockage of the system;

The advantage is that all cells have the same microenvironment, and there is a large amount of cell culture space, which reduces the occurrence of immune reactions. The disadvantage is that the cell stability is poor, the material exchange capacity is limited, and the system is easy to block

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