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Sertraline detection reagent, and preparation and use methods thereof

A detection reagent and sertraline technology, which is applied in the field of sertraline detection reagents and can solve problems such as complicated operation

Active Publication Date: 2019-11-15
长沙博源医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, the commonly used methods for detection of sertraline are gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography (HPLC) and enzyme-linked immunoassay (ELISA), etc. There are certain limitations in promotion and application
At present, there is a lack of sertraline detection reagents with good stability, high sensitivity and strong specificity in the market, especially high-throughput automated detection reagents with good quality

Method used

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  • Sertraline detection reagent, and preparation and use methods thereof
  • Sertraline detection reagent, and preparation and use methods thereof
  • Sertraline detection reagent, and preparation and use methods thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1. the synthesis of sertraline maleimide derivative

[0063] The chemical structural formula of sertraline maleimide derivatives is shown in formula (Ⅲ):

[0064]

[0065] Formula (Ⅲ).

[0066] The synthetic route and preparation steps of above-mentioned sertraline maleimide derivatives are as follows:

[0067]

[0068] Concrete synthetic steps are as follows:

[0069] (a) Synthesis of Compound 2: Weigh 7.8 g of Compound 1 and dissolve in 100 ml of trifluoroacetic acid, then add 7 ml of trifluoromethanesulfonic acid and 2.3 g of potassium nitrate to prepare a reaction mixture, stir at room temperature for 1.5 hours, and Add 100ml of purified water to the reaction mixture, then extract twice with 100ml of ethyl acetate, wash the extracted organic matter with 200ml of brine, wash with MgSO 4 Filter after drying, then remove the solvent by reducing pressure, and finally obtain 3.8 g of compound 2 through silica gel purification to obtain the crude produ...

Embodiment 2

[0084] Example 2. Preparation of Sertraline Immunogen

[0085] The sertraline immunogen in this example is formed by linking the sertraline maleimide derivative represented by formula (III) with methylated bovine serum albumin (MBSA), and its structural formula is as follows: formula (V) Shown:

[0086]

[0087] Formula (Ⅴ).

[0088] The concrete steps of the synthetic method of this sertraline immunogen are as follows:

[0089] (1) Dissolve MBSA with a mass fraction of 1.25% in 0.3 mmol / L, pH=8.0 phosphate buffer to obtain MBSA solution;

[0090] (2) Dissolve 0.8% sertraline maleimide derivatives, 7.5% dimethylformamide, and 7.5% ethanol in 20 mmol / L, pH=5.2 potassium phosphate buffer, Then add 1-ethyl-3-(-3-dimethylaminopropyl)carbodiimide with a mass fraction of 0.8%, and stir and react the above chemical substances at 0°C for 120 minutes;

[0091] (3) Slowly add the solution dissolved in step (2) to the MBSA solution in step (1) dropwise, and stir at -8°C for 48 hou...

Embodiment 3

[0092] Example 3. Preparation of anti-sertraline specific antibody

[0093] The sertraline immunogen prepared in Example 2 was inoculated with experimental animal goats by conventional methods, and the antiserum was taken after booster immunization. The specific steps were as follows:

[0094] a. Dilute the sertraline immunogen to 2.5 mg / ml with PBS buffer to obtain an immunogen solution, then mix 2.5 ml of the immunogen solution with an equal amount of Freund's complete adjuvant, and inject the experimental animal goat;

[0095] b. After 2 weeks, mix 2.5 ml of the same immunogen solution with the same amount of Freund's incomplete adjuvant, inject once into the above-mentioned experimental animal goat, and then inject once every 4 weeks, a total of 5 injections;

[0096] c. Blood was collected from the immunized experimental animal goat, and the anti-sertraline specific antibody was obtained by separation and purification, and its titer was determined to be 1:12500.

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Abstract

The invention relates to a sertraline detection reagent, and preparation and use methods thereof. Through repeated experiments, a bran-new sertraline maleimide derivative is obtained; the sertraline maleimide derivative is used for preparing a sertraline immunogen with high immunogenicity; further, experiment animals are immunized to obtain a high-potency anti-sertraline specific antibody; and meanwhile, the sertraline maleimide derivative is used for preparing a sertraline enzyme conjugate. The automatic determination of the sertraline content on a full-automatic biochemical analyzer can be realized by the sertraline detection reagent containing the anti-sertraline specific antibody and the sertraline enzyme conjugate; the content of sertraline in a biological sample can be fast and precisely determined at high flux; the advantages of operation simplicity and convenience, high sensitivity, high specificity, high result accuracy and the like are realized; the sertraline detection costis effectively reduced; and the wide popularization and use in clinics are facilitated.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a sertraline detection reagent and a preparation and use method thereof. Background technique [0002] Sertraline (Sertraline), its chemical structural formula is shown in formula (IV): [0003] [0004] Formula (Ⅳ) [0005] Sertraline is a potent and selective inhibitor of neuronal serotonin reuptake. At clinical doses, sertraline blocks the uptake of serotonin by human platelets and is only sensitive to norepinephrine and dopamine. weak impact. In vitro studies have shown that sertraline has effects on adrenergic receptors (α1, α2, β), cholinergic receptors, GABA receptors, dopaminergic receptors, histamine receptors, serotonergic receptors (5HT1A, 5HT1B, 5HT2) or benzodiazepine receptors with no apparent affinity. Antagonism of these receptors is thought to be associated with the sedative, anticholinergic, and cardiotoxic effects of other psychi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/00C07K14/77C07K14/765C07K14/795C07K14/47C07K19/00C07K16/44C07D207/452
CPCG01N35/00C07K14/77C07K14/765C07K14/795C07K14/47C07K19/00C07K16/44C07D207/452G01N2035/00465
Inventor 崔丽艳虞留明
Owner 长沙博源医疗科技有限公司
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