Genetically engineered bacterium for producing L-valine with high yield and establishment method and application of genetically engineered bacterium

A technology of genetically engineered bacteria and valine, applied in the field of microorganisms and molecular biology, can solve the problems of low L-valine yield, insufficient supply of pyruvate, weak metabolic flow, etc.
CN110468092AActive Publication Date: 2019-11-19TIANJIN UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TIANJIN UNIV OF SCI & TECH
Publication Date
2019-11-19

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Abstract

The invention provides a genetically engineered bacterium for producing L-valine with high yield, which is established by changing the metabolic pathway of escherichia coli. Starting from escherichiacoli W3110, an encoding gene alsS of acetolactate synthase of bacillus subtilis is integrated on a genome of the escherichia coli W3110 and is intensively expressed; in addition, an encoding gene spoTof an escherichia coli ppGpp 3'-pyrophosphoric acid hydrolase mutant [R290E, K292D] is integrated on the genome of the escherichia coli W3110 and is intensively expressed; and furthermore, an encoding gene thiE of thiamine phosphate synthase is knocked off, and thus the genetically engineered bacterium is established. By adopting the genetically engineered bacterium, the problem that L-valine islow in yield as L-valine is weak in synthesis metabolic flux and a key precursor pyruvic acid is insufficient in supply can be solved.
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Description

technical field

[0001] The invention belongs to the field of microbiology and molecular biology, and relates to a genetically engineered strain of high-yield L-valine and its construction method and application. Background technique

[0002] L-valine belongs to branched-chain amino acid and is an essential amino acid for human body. L-valine is widely used in the fields of medicine, food and feed, and has high commercial value. L-valine can be used as an antitumor drug in medicine and a food additive in food. In addition, L-valine has become an important feed amino acid.

[0003] At present, the production method of L-valine mainly adopts the microbial fermentation method with glucose as raw material. The L-valine synthesis branch pathway starts from pyruvate and undergoes the following steps to form L-valine. Pyruvate is catalyzed by acetolactate synthase to form α-acetolactate, and α-acetolactate is catalyzed by dicarboxylic acid reductoisomerase to form α, β-dihydroxy...

Claims

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