Method of identifying reconstituted milk and ultra-high temperature sterilized milk

A technology for ultra-high temperature sterilization and reconstituted milk, which is applied in the field of identification of reconstituted milk and ultra-high temperature sterilized milk. It can solve problems such as false positives, cumbersome operations, and complicated pretreatments, and achieve high accuracy, strong feasibility, and high sensitivity. Effect

Active Publication Date: 2019-11-19
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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Problems solved by technology

In the existing national standard method, the content of furosine and lactulose in liquid milk is detected by high performance liquid chromatography and enzymati

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  • Method of identifying reconstituted milk and ultra-high temperature sterilized milk
  • Method of identifying reconstituted milk and ultra-high temperature sterilized milk
  • Method of identifying reconstituted milk and ultra-high temperature sterilized milk

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preparation example Construction

[0081] 1) Milk sample preparation

[0082] Preparation of milk samples: reconstituted milk is prepared by dissolving whole milk powder in water, with a protein content of 3.0g / 100mL. Ultra-high temperature sterilized milk (UHT) is commercially available; the dairy product is sampled and centrifuged, the lower layer liquid is taken and dissolved in acetonitrile, and then the upper layer solution is separated by centrifugation. After passing through a 0.22 μm filter membrane, the milk sample is obtained and put on the machine for testing.

[0083] 2) Data collection and preprocessing

[0084] Use a high-resolution quadrupole time-of-flight mass spectrometer to detect milk samples of reconstituted milk and ultra-high temperature sterilized milk, and collect raw data; preprocess and analyze the raw data, perform baseline correction, noise removal, and retention time calibration , Identification of chromatographic peaks, peak matching, obtaining a two-dimensional data matrix, incl...

Embodiment 1

[0088] Collection and preparation of milk samples

[0089] 30 brands of ultra-high temperature sterilized milk and 30 brands of whole milk powder (milk powder) were collected, all of which were purchased from Beijing and Hulunbuir. Among them, the purchased whole milk powder is dissolved in water, and the reconstituted milk is prepared according to the protein content of 3.0g / 100mL.

[0090] The preparation method of the reconstituted milk is as follows: according to the protein content of 3.0g per 100mL of milk, the milk powder is added to the ultrapure water, and the temperature is kept in a water bath to 40°C, and the reconstituted milk is homogenized by hand to obtain a milk sample of the reconstituted milk.

[0091] Preparation of milk samples to be tested: Take 2 mL each of the above ultra-high temperature sterilized milk and reconstituted milk, centrifuge at 8000 rpm for 30 min to remove lipids with large molecular weight; separate the lower layer solution, take 1 mL,...

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Abstract

The invention relates to a method of identifying reconstituted milk and ultra-high temperature sterilized milk. The method comprises steps: a) non target metabonomics data of milk samples of reconstituted milk and ultra-high temperature sterilized milk are collected separately by adopting high performance liquid chromatography-high resolution mass spectrometry, and the characteristic information of the two is extracted respectively; b) an analysis model is built, the characteristic information is analyzed, and differential metabolites of the reconstituted milk and the ultra-high temperature sterilized milk are determined; c) a pseudo-target metabolomics detection method to identify reconstituted milk and ultra-high temperature sterilized milk is constructed to screen the differential metabolites in the step b), and the characteristic information of the differential metabolites is obtained; and d) based on the characteristic information of the differential metabolites obtained in the step c), a PCA-Class discriminant analysis model is constructed, and based on the PCA-Class discriminant analysis model, determination of a to-be-detected milk sample is realized. The method is convenient, sensitive and accurate, and can realize rapid and automatic discrimination of ultra-high temperature sterilized milk and reconstituted milk.

Description

technical field [0001] The invention relates to the field of instrument analysis and food inspection, in particular to a method for identifying reconstituted milk and ultra-high temperature sterilized milk. Background technique [0002] Reconstituted milk, also known as "reconstituted milk" or "reconstituted milk", refers to milk that is concentrated and dried to become concentrated milk or milk powder, and then add an appropriate amount of water to make an emulsion that is equivalent to the ratio of water and non-fat solids in raw milk. Since the reconstituted milk is blended with milk powder, after the milk powder is processed at high temperature, the nutrients in terms of amino acids, proteins, vitamins and sugars have a greater loss compared with raw milk, especially some vitamins, immunoglobulins, etc. , Lactoferrin, sulfur-containing amino acids, lysine, etc. are seriously lost, while the nutrients in pure milk are basically preserved. [0003] The current practice of...

Claims

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Application Information

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IPC IPC(8): G01N30/86G01N30/34
CPCG01N30/34G01N30/8679
Inventor 陈刚谭冬飞朱丹贾曼苏美丞王少雷
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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