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Feather meal additive, preparation method and application

A feather meal and additive technology, which is applied in the application and introduction of foreign genetic material and hydrolase using a carrier, can solve problems such as hidden safety hazards and major amino acid damage, and achieve the effects of cleanliness, energy consumption, high solid content and high concentration.

Inactive Publication Date: 2019-11-29
HUAZHONG AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has certain hidden dangers in the operation process, and it greatly damages the amino acid in the feather meal, and the final crude protein content is only 90%.

Method used

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  • Feather meal additive, preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Pichia pastoris AN-PK-9K-GS115 described in this embodiment is selected and bred by the following method:

[0039] A, take Bacillus subtilis as the original gene source;

[0040] B. Obtain the target gene sequence Fpk by using overlap extension PCR technology; the overlap extension PCR technology uses primers with complementary ends to make the PCR products form overlapping chains, so that in the subsequent amplification reaction, the overlapping chains can be extended to separate the genes from different sources. The amplified fragments are overlapped and spliced ​​together;

[0041] The conditions during the PCR amplification process are as follows: (containing 10 times buffer and Taq enzyme, dNTP and other In-Fusion kits) to obtain the target gene sequence Fpk (as shown in SEQ ID NO: 1);

[0042]Primer F: 5'-ctcctgccaagctgaagc-3',

[0043] Primer R: 5'-gatcatggaacggattc-3',

[0044] Amplification program: 95°C 2min; 95°C 30s, 56°C 30s, 72°C 1min, 45 cycles; 72°C 5...

example 2

[0055] Soak the above-mentioned feather meal in NaOH solution, solid-state enzymolysis for 3 hours under the conditions of material-water ratio 1:0.5, enzyme activity 1500U / g, temperature 65°C, and pH 9.0, to obtain an enzymolysis reaction solution. After testing, 55% total The protein is hydrolyzed into soluble protein, 46% of which is acid-soluble protein, and the content of ammonia nitrogen is 2.2%. The feather meal additive is obtained after the enzymolysis reaction solution is concentrated and dried.

example 3

[0057] Feather powder is soaked in NaOH solution, the ratio of material to water is 1:0.5, enzyme activity is 1500U / g, temperature is 65°C, pH is 9.0, Mg +2 Under the condition of 2mM solid-state enzymatic hydrolysis for 3 hours, the enzymatic hydrolysis reaction solution was obtained. After testing, 61% of the total protein was enzymatically hydrolyzed into soluble protein, 52% of which was acid-soluble protein, and the content of ammoniacal nitrogen was 2.7%. The feather meal additive is obtained after the enzymolysis reaction solution is concentrated and dried.

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Abstract

The invention discloses a feather meal additive, a preparation method and application and belongs to the technical field of feed additive processing. The additive is prepared by the following steps: (1) preparing feather meal; (2) performing solid enzymolysis; and (3) performing filtering and drying. By adopting the method, livestock feather is adopted as a raw material which is crushed, a solid enzymolysis mode is adopted, the feather meal is adopted as the raw material, the material to water ratio is 1:0.5, the pH value is adjusted to 9.0 by using lime, the temperature is 55 DEG C, solid enzymolysis is implemented for 24 hours, and finally filtering and drying are implemented to prepare the additive. The additive is simple in operation process, low in enzymolysis temperature and wide inpH value action range, and the use fields of the process can be widened; the additive is low in enzymolysis cost and high in enzymolysis efficiency, enzymolysis supernate is high in soluble protein content and meanwhile comprises multiple amino acid components of high contents, the production cost can be lowered, comprehensive economic benefits can be increased, requirements of industrial production can be met; and the additive can be applied to feeds.

Description

technical field [0001] The invention belongs to the technical field of preparation of animal feed additives, and in particular relates to a feather meal additive, a preparation method and an application thereof. Background technique [0002] The poultry breeding industry is one of the pillar industries of my country's animal husbandry industry. The output of chicken, duck and goose feathers is very rich, with an annual output of more than one million tons. Therefore, feather meal is a commonly used protein feed raw material. Its crude protein is more than 80%, rich in various essential amino acids for animals, the sum of amino acids is more than 70%, and the content of sulfur-containing amino acids is high. It has high utilization value in feed , but because the disulfide bond protein structure of feathers is difficult to be digested and absorbed by animals, which seriously affects its development and utilization, so it is very important to make full use of feather protein r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/14A23K10/26C12N9/60C12N15/81
CPCA23K10/14A23K10/26C12N9/60C12N15/815
Inventor 彭楠刘玲常章兵田建平梁运祥
Owner HUAZHONG AGRI UNIV
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