Microbial inoculant for controlling prodenia litura larvae, preparation method and application thereof

Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a microbial inoculant for controlling prodenia litura larvae, and a preparation method and an application thereof. The microbial inoculant for controlling the prodenia litura larvae of the present invention includes the following components: metarhizium anisopliae, Bacillus thuringiensis bacillus, P. stewari, and Clostridium sporogenes. The microbial inoculant is prepared by mixing bacteria after activation, expansion culture and fermentation culture. With a volume ratio of 45% of metarhizium anisopliaebacteria liquid, 29% of Bacillus thuringiensis bacteria liquid, 18% of Pantoea sclerotiorum bacteria liquid, and 8% of Clostridium sporogenes bacteria liquid, the microbial inoculants has the highestmortality rate on the prodenia litura larvae. The microbial inoculant prepared by the present invention has high insecticidal virulence and good fast-acting properties. The four inoculant componentswith completely different action mechanisms are compounded to increase target sites and make insects less prone to resistance; the persistent period is low, the cost is lower, and the product has theadvantages of safety and environmental protection.

Description

technical field [0001] The invention belongs to the field of biotechnology prevention and control, and in particular relates to a microbial agent for preventing and controlling Spodoptera litura larvae, a preparation method and application thereof. Background technique [0002] Spodoptera litura is a kind of omnivorous and gluttonous pest, which has a wide range of harm. In addition to cruciferous vegetables, it can also harm nearly 100 families and more than 300 species including melons, eggplants, beans, onions, leeks, spinach, grains, and economic crops. plant. Spodoptera litura has been controlled by chemical pesticides for many years. [0003] Chemical pesticide control has played a positive role in quickly controlling the occurrence and spread of pests. However, chemical pesticide control against Spodoptera litura has disadvantages such as high cost, large dosage, and poor safety. Extensive use can easily cause human and animal poisoning, pollute the grassland, and ki...

AI Technical Summary

Problems solved by technology

[0004] In view of this, the purpose of the present invention is to provide a microbial agent for preventing and controlling Spodoptera litura larvae and its preparation method and application, so as to solve the problems of high cost, poor safety, serious pollution, etc. that chemical pesticides exist in preventing and controlling Spodoptera litura

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