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Magnetic particle chemiluminiscence kit for measuring content of prealbumin in human body and preparation method thereof

A technology of prealbumin and chemiluminescence, which is applied in the field of magnetic particle chemiluminescence detection kit, magnetic particle chemiluminescence detection kit and its preparation, can solve the problems of low sensitivity, narrow linear range, unstable results, etc. Low processing requirements, simple operation, and the effect of ensuring sensitivity

Pending Publication Date: 2019-12-03
BEIJING LEADMAN BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods still have defects such as low sensitivity, narrow linear range, and unstable results.

Method used

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  • Magnetic particle chemiluminiscence kit for measuring content of prealbumin in human body and preparation method thereof
  • Magnetic particle chemiluminiscence kit for measuring content of prealbumin in human body and preparation method thereof
  • Magnetic particle chemiluminiscence kit for measuring content of prealbumin in human body and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Preparation of a magnetic particle chemiluminescence detection kit for measuring the content of human prealbumin

[0048] The kit includes R1 reagent, R2 reagent, magnetic separation reagent, calibrator liquid series and chemiluminescence substrate liquid.

[0049] Among them, R1 reagents include: 1) R1 antibody: Fluorescein isothiocyanate (FITC) labeled anti-human prealbumin monoclonal antibody at a concentration of 1 μg / ml; 2) buffer: including Tris at a concentration of 12.0 g / L; sodium azide, the concentration is 1.98g / L; sodium chloride, the concentration is 5.9g / L; fish skin gelatin, the concentration is 10g / L; the rest is deionized water. The buffer pH of the R1 reagent is 8.0.

[0050] Among them, the R2 reagents include: 1) R2 antibody: alkaline phosphatase-labeled anti-human prealbumin antibody at a concentration of 0.5 μg / ml; 2) buffer: a commercial AP Conjugate Stabilizer. The buffer pH of R2 reagent is 8.0.

[0051] Among them, the magnetic separation ...

Embodiment 2

[0054] Example 2 Preparation and determination method of magnetic particle chemiluminescence detection kit for measuring human prealbumin content

[0055] (1) First, compare the 50μl calibrator series (concentrations of 0mg / dL, 1.5mg / dL, 3.5mg / dL, 7mg / dL, 20mg / dL, 60mg / dL) of Example 1 with those of Example 1. Add 50μl reagent R1 and 50μl reagent R2 to the reaction tube in sequence, and incubate for 15min at 37℃;

[0056] (2) Combine the above reagent series with 25μl magnetic separation reagent of Example 1 and continue incubating at 37°C for 5 minutes;

[0057] (3) Wash 3 times with a cleaning solution to remove unbound antibodies and impurities;

[0058] (4) Add 150μl of the luminescent substrate solution of Example 1, and use Leadman's self-developed chemiluminescence detector to measure the relative luminescence intensity (RLU) after ALP catalyzes the luminescence of the substrate. The results are shown in Table 1 below:

[0059] Table 1

[0060] Calibrator (mg / dL)01.53.572060...

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Abstract

The invention discloses a magnetic particle chemiluminiscence kit for measuring the content of prealbumin in a human body and a preparation method thereof. The kit comprises a reagent R1, a reagent R2, a magnetic separation reagent, a calibrator solution series and a chemiluminescence substrate solution which are used for measuring the prealbumin of the human body. With a reaction mode of a sandwich method, the prealbumin content of a human body in a human serum sample is detected quantitatively according to a principle of combining the chemiluminescence detection technology with the magneticparticle immune separation technology, so that the detection sensitivity is ensured. Compared with the traditional kit, the disclosed kit has advantages of being high in sensitivity, free of pollution, high in specificity, easy to operate, low in sample pretreatment requirement; a large number of samples can be detected rapidly in a high-throughput mode; and clinical reagent application is facilitated. According to the invention, an accurate, convenient, rapid and simple method for clinically detecting the humanprealbumin in the human serum is provided.

Description

Technical field [0001] The invention relates to a magnetic particle chemiluminescence detection kit and a preparation method thereof, in particular to a magnetic particle chemiluminescence detection kit which adopts magnetic particle chemiluminescence technology and antigen-antibody combination technology to detect the content of human prealbumin. The invention belongs to the field of medical diagnostic reagents. Background technique [0002] Prealbumin (PA) is a tryptophan-rich serum negative phase protein with a molecular weight of 54980 synthesized by the liver, also known as thyroxine transporter. The main role of PA is to participate in the transport of thyroxine, and at the same time as a retinol binding protein to transport vitamin A indirectly, and has the activity of thymus hormone, which can increase human immunity by promoting the maturation of lymphocytes. It consumes itself while removing toxic metabolites, and the more severe the inflammation, the more serum PA is ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/543G01N33/58G01N21/76
CPCG01N33/6893G01N33/54326G01N33/54393G01N33/582G01N33/581G01N21/763G01N2800/085
Inventor 王国武郭健夫胡少达
Owner BEIJING LEADMAN BIOCHEM
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