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Method for replacing yeast powder with soybean meal enzymolysis liquid during threonine production

A technology of soybean meal enzymatic hydrolysate and threonine, which is applied in the direction of fermentation, can solve the problem of high cost of threonine fermentation

Inactive Publication Date: 2019-12-10
卢松
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the defect of high threonine fermentation cost in the prior art, and provide a method for replacing yeast powder with soybean meal enzymatic hydrolyzate in threonine production

Method used

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  • Method for replacing yeast powder with soybean meal enzymolysis liquid during threonine production

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Experimental program
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Embodiment 1

[0021] A method for replacing yeast powder with soybean meal enzymatic hydrolysis solution in threonine production, comprising the following steps:

[0022] 1) Preparation of soybean meal enzymatic hydrolysis solution:

[0023] Mix soybean meal and water at a mass ratio of 1:1, steam at 121°C for 10 minutes, and then naturally cool to room temperature to obtain a soybean meal culture solution; the seed solution of Bacillus licheniformis, Cellulomonas flavinogenes, and Aspergillus niger And the aspergillus oryzae seed liquid is mixed according to the volume ratio of 2:2:1:1 to obtain the mixed seed liquid; then the mixed seed liquid is inserted into a fermenter containing soybean meal culture liquid according to 10% inoculation amount to carry out fermentation to produce enzyme, The temperature is 35°C, the tank pressure is 0.03MPa, the air volume is 500L / h, the fermentation enzyme production time is 60h, ultrasonic treatment, the ultrasonic frequency is 25kHz, the ultrasonic t...

Embodiment 2

[0041] The components of soybean meal produced by various soybean species are not very different. The present invention selects the soybean meal with skin, and the contents of each main component are: protein 45%, ash content 7%, fiber 20%, moisture 10%, fat 2%, carbohydrate 14%, the others are inorganic minerals.

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Abstract

The invention belongs to the technical field of amino acid production, and discloses a method of replacing yeast powder with soybean meal enzymolysis liquid during threonine production. The method comprises the following steps: (1) preparing of the soybean meal enzymolysis liquid, and (2) strain fermentation. According to the method, the soybean meal enzymolysis liquid is adopted to replace yeastextract in a culture medium, compared with the mode of adopting the yeast extract, the threonine output and dry matter in fermentation liquor are both increased, and the cost is relatively low.

Description

technical field [0001] The invention belongs to the technical field of amino acid production, and relates to a method for replacing yeast powder with soybean meal enzymatic hydrolysis solution in threonine production. Background technique [0002] Threonine (Threonine, abbreviated as Thr), scientific name 2-amino-3-hydroxybutyric acid, belongs to aliphatic amino acid, slightly sweet, named for its structure similar to threose, is a kind of human, animal and plant protein Essential amino acids are mainly used in medicine, chemical reagents, and nutritional enhancers. They can strengthen dairy products, restore human fatigue, and promote growth and development. In recent years, with the development of the economy, the market demand for threonine has continued to grow steadily, and it is one of the amino acid varieties with the fastest growing demand. Especially, the use in chemistry and biochemistry, food additives, feed additives, etc. has grown rapidly. It replaces tryptoph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/08
CPCC12P13/08
Inventor 李学朋伏广好许传娟梁晓娟王文强郄子玥张建国周敬
Owner 卢松