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Bacillus velezensis CY30 in prevention and treatment of pestalotiopsis theae and application thereof

A technology of bacillus and tea leaf spot, applied in the field of agricultural microorganisms, can solve the problems of continuous cropping tea tree decline, bacteria resistance, soil acidification, etc., and achieve the reduction of chemical fertilizers and pesticides, no residue and pollution, and persistent bactericidal activity Effect

Active Publication Date: 2019-12-13
WUHAN CHUQIANG BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of chemical fungicides is still the main means of controlling tea leaf spot, but the application of chemical fungicides can easily lead to bacterial resistance, and at the same time cause pesticide residues and environmental pollution
[0004] In tea planting, a large amount of chemical fertilizers are usually used in order to stabilize and increase production. Long-term application of chemical fertilizers will cause soil compaction, insufficient absorption and utilization of tea nutrients, and acidification of the soil, which will accelerate the decline of continuous cropping tea trees, resulting in reduced tea production. Need to solve the above problems in tea production

Method used

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  • Bacillus velezensis CY30 in prevention and treatment of pestalotiopsis theae and application thereof
  • Bacillus velezensis CY30 in prevention and treatment of pestalotiopsis theae and application thereof
  • Bacillus velezensis CY30 in prevention and treatment of pestalotiopsis theae and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Acquisition and Identification of Bacillus Velez CY30:

[0022] Bacillus Velez CY30, isolated from the rhizosphere soil of tea trees in Hefeng County, Enshi Prefecture, Hubei Province, was screened for antibacterial activity against tea leaf spot. The taxonomic status of the strain was further confirmed by 16s rDNA and gyrA gene sequencing combined with physiological and biochemical identification (Table 1, Table 2). It was sent to the China Center for Type Culture Collection on October 25, 2018 for preservation. The taxonomic designation: Bacillus velezensis CY30; deposit number CCTCC NO:M2018710; place: Wuhan University, Wuhan, China.

[0023] In the present invention, Bacillus velezensis CY30 may be referred to as CY30 for short.

[0024] Table 1 Physiological and biochemical characteristics of strain CY30-enzyme activity, carbon source oxidation

[0025]

[0026]

[0027] +: Positive reaction; -: Negative reaction; W: Weak positive reaction

[0028] Table 2...

Embodiment 2

[0033] Fermentation of Bacillus Velez CY30 (percentages in the following medium formula are mass fractions):

[0034] 1) First-level seed culture: 200ml of first-level seed medium is placed in a 500ml triangular flask, sterilized by damp heat at 121°C for 30 minutes, and inoculated into the first-level seed medium by taking Bacillus Velez CY30 freeze-dried tube strains. Cultivate at 30°C-35°C for 8-10h; the raw materials and dosage of the medium used for primary seed cultivation are (mass fraction): glucose 2%, beef extract 2%, peptone 1%, sodium chloride 0.35%, medium pH is 7;

[0035] 2) Secondary seed culture: 240L secondary seed culture medium is installed in a 400L fermenter, sterilized by moist heat at 121°C for 30 minutes, and 200ml of primary seed is inoculated in the secondary seed medium; cultured at 35°C for 10h; control tank The pressure is 0.05Mpa, the stirring speed is 150rpm, the air ratio is 1:0.8, the raw materials and dosage of the medium used for secondary ...

Embodiment 3

[0039] Application of Bacillus Velez CY30 in the prevention and treatment of tea leaf spot:

[0040] The tea leaf spot (Pestalotiopsis theae) was activated on the PDA plate for 5 days before use, and the tea leaves (Zhongcha 108) of the same size without using fungicides were collected for the detached leaf test. Prepare CY30 fermentation broth according to Example 2, the number of spores is about 10 billion cfu mL -1 , using sterile water to dilute the fermentation broth 5 times and 10 times respectively for later use. Use an electric sprayer to evenly spray 5-fold dilutions and 10-fold dilutions of the fermentation broth on the tea leaves, 2 mL per leaf, and spray sterile water for the blank control. After each treatment was air-dried, acupuncture inoculation method was used to inoculate tea leaf blotch fungus cake, 28°C moisture culture for 12 days, the control and treatment lesion diameters were counted and the control effect was calculated. Each treatment was repeated 3 ...

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Abstract

The invention belongs to the field of agricultural microorganisms, and discloses a bacillus velezensis CY30 and an application thereof. The bacillus velezensis is bacillus velezensis CY30, and a preservation number is CCTCC NO: M2018710. The strain is reported for the first time to be used for preventing and treating pestalotiopsis theae of tea trees by foliage spray, root irrigation has the effects of promoting growth and increasing yield of the tea trees, and the strain has a wide application prospect in agricultural production.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and in particular relates to a strain of Bacillus velezensis CY30 and its application in the prevention and treatment of tea leaf spot. Background technique [0002] Tea tree is an important economic crop in my country. Since the founding of New China, the tea industry has developed rapidly. According to FAO statistics, as of 2017, the area of ​​tea gardens was 2.22×10 6 ha, yield 2.47×10 6 tons, the area of ​​tea gardens and the total output of tea both rank first in the world. Tea has played a huge role in industrial poverty alleviation and foreign exchange earning in poverty-stricken mountainous areas in my country. [0003] Tea trees mostly grow in warm and humid tropical and subtropical regions, and the climate in these regions is also conducive to the growth of tea tree diseases. Tea wheel spot is an important disease of tea. It has been reported in tea planting areas all over...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 黄大野曹春霞刘芳杨丹姚经武廖先清饶犇周荣华
Owner WUHAN CHUQIANG BIOLOGICAL TECH CO LTD
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