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Cortex albiziae lignan glycoside compound promoting endothelial cell proliferation and application

A technology of endothelial cell proliferation and lignan glycosides, which is applied in the field of lignan glycosides from Albizia Julibis Bark, can solve the problems of difficult extraction and purification process, unclear pharmacological activity of chemical components, and low content of lignans. Effects of promoting cell migration, promoting wound healing, and promoting migration

Pending Publication Date: 2020-01-03
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some literatures show that the content of lignans in the bark of Albizia juliensis is low, which has caused certain difficulties for the comprehensive evaluation of the extraction and purification process of the water-soluble lignans in the bark of Albizia juliensis
At the same time, the skin of Albizia Julibrissin is often used as a component of a compound drug, and is rarely used alone, so the pharmacological activities of various chemical components contained in it are still unclear

Method used

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  • Cortex albiziae lignan glycoside compound promoting endothelial cell proliferation and application
  • Cortex albiziae lignan glycoside compound promoting endothelial cell proliferation and application
  • Cortex albiziae lignan glycoside compound promoting endothelial cell proliferation and application

Examples

Experimental program
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Effect test

Embodiment 1

[0024] The preparation of embodiment 1 compound

[0025] (1) Extraction Take 20 kg of dried Albizia juliensis skin, pulverize it, use 5 times of 75% ethanol (water), 100 L each time, and extract twice at 80° C. for 2 hours each time. The residue of the bark of Albizia Julibrissin was removed by filtration, the 75% ethanol extract of the bark of Albizia Julibis was combined, and freeze-dried to obtain 1.6 kg of crude ethanol extract of the bark of Albizia Julibis. Grind the crude extract and suspend it in 2L deionized water to dissolve it as much as possible. After suspension, extract with ethyl acetate and saturated n-butanol sequentially. The extraction adopts the principle of adding a small amount of ethyl acetate and saturated n-butanol each time, and the extraction is terminated when the color of the extract becomes light to colorless. Extract the ethyl acetate phase and the saturated n-butanol phase to obtain the extracts of the ethyl acetate part and the n-butanol part....

Embodiment 2

[0038] Example 2 Icaritin E 5 Promote endothelial cell proliferation activity

[0039] (1) Human umbilical vein endothelial cells (HUVECs) were cultured in DMEM medium containing 10% FBS and 1% penicillin / streptomycin in a constant temperature incubator at 37°C containing 5% CO2. Change the medium every 1-2 days and passage cells that are 85-90% confluent at a 1:3 confluent ratio. In all experiments, cells were used between passage 2 and passage 5. HUVEC cells in the logarithmic growth phase were taken, and 96-well plates were plated, 100 μL of medium was added to each well, and 3000 cells were plated. After 12 hours of plating, the cells were adhered to the wall and administered, continued to culture for 24 hours, then added 10 μL CCK-8 to each well, incubated for 1 hour at 37°C in a cell incubator, and detected the absorbance (OD) value at a wavelength of 450 nm with a microplate reader. The result is as Figure 6 As shown, compared with the blank control group, after 24...

Embodiment 3

[0041] Example 3 Icaritin E 5 Effects on HUVEC cell migration.

[0042] Human umbilical vein endothelial cells (HUVECs) were cultured in DMEM medium containing 10% FBS and 1% penicillin / streptomycin in a constant temperature incubator at 37°C containing 5% CO2. Change the medium every 1-2 days and passage cells that are 85-90% confluent at a 1:3 confluent ratio. In all experiments, cells were used between passage 2 and passage 5. Take HUVEC cells in the logarithmic growth phase, spread 6-well plates, 200,000 per well, culture for 24 hours, that is, after the cells adhere to the wall, scratch the cells in the dish, then wash twice with PBS, add medium (containing 40 μM Icariside E 5 Monomer compound) was cultured for 24 hours, and the state of cell migration was observed. The experimental results showed that icariside E 5 Can significantly promote cell migration, the results are as follows Figure 7 As shown, after scratching, administer 40μM and continue to culture for 2...

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Abstract

The invention discloses a cortex albiziae lignan glycoside compound promoting endothelial cell proliferation and application, and belongs to the technical field of biomedicine. New application of Icarisid E5 in promoting the proliferation and migration of endothelial cells and promoting angiogenesis is provided. It is confirmed by experiments that the HUVEC cell viability after administration of 40 [mu]M for 24 h can be increased to 133.65% of the control, cell migration can be significantly promoted, and the function of promoting wound healing in in-vivo experiments is achieved. The preparedmonomeric compound Icarisid E5 can significantly promote cell proliferation activity, promote the migration and self-replication of HUVEC cells, can be used for transferring exogenous angiogenesis-inducing factors into tissue, promotes angiogenesis, and enhances cell reproductive capacity.

Description

technical field [0001] The invention relates to an albizia bark lignan glycoside compound for promoting the proliferation of endothelial cells and its application, belonging to the technical field of biomedicine. Background technique [0002] Albiziae Cortex is the bark of Albzia julibrissin Durazz, a leguminous plant. It is a commonly used traditional Chinese medicine. In recent years, with the in-depth research of Albizia Julibrissin by scholars, its chemical composition and pharmacological and pharmacological activities have been continuously discovered. [0003] So far, a variety of compounds have been isolated from Albizia Julibrissinus, including triterpenes, flavonoids, lignans and so on. It has been shown in the literature that the content of lignans in Albizia Julibrissin is relatively low, which makes it difficult to comprehensively evaluate the extraction and purification process of water-soluble lignans in Albizia Julibis Bark. At the same time, Albizia Julibri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7034A61P9/00A61P35/00A61P17/02A23L33/105A23L33/125
CPCA61K31/7034A61P9/00A61P35/00A61P17/02A23L33/105A23L33/125A23V2002/00A23V2200/326A23V2250/21
Inventor 邱丽颖史学林倪露露周跃涛
Owner JIANGNAN UNIV
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