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Fish scale collagen peptide and preparation method thereof

A technology of fish scale collagen and collagen peptides, which is applied in the preparation method of peptides, chemical instruments and methods, animal/human proteins, etc., can solve the problems of destroying soil structure, ineffective degradation and landfill, etc., and increase the contact area Large, save time and labor costs, small molecular weight effect

Inactive Publication Date: 2020-01-03
汕尾市维明生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the production of fish collagen peptides must be treated with acid and alkali, and at the same time, activated carbon and perlite are used for decolorization. The mixture of activated carbon and perlite after decolorization destroys the soil structure and cannot be effectively degraded and landfilled, causing various environmental problems.

Method used

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  • Fish scale collagen peptide and preparation method thereof
  • Fish scale collagen peptide and preparation method thereof
  • Fish scale collagen peptide and preparation method thereof

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preparation example Construction

[0024] The invention provides a preparation method of fish scale collagen peptide, which comprises the following steps: (1) mixing fish scales, water and lipase, and then lipidizing for 20 to 30 minutes, and obtaining lipidated fish scales after deactivating the enzyme;

[0025] (2) Mixing the fatty fish scales with water and then performing wall-breaking treatment to obtain liquid fish scales; the particle size of the liquid fish scales is 20-60 mesh;

[0026] (3) mixing the liquid fish scales with protease, enzymolyzing them for 3.5-4 hours, centrifuging after inactivating the enzymes, and obtaining collagen serum;

[0027] (4) decolorize after mixing the collagen serum with the first decolorizing agent to obtain semi-decolorized collagen peptide; include activated carbon and wood fiber in the first decolorizer, and the weight ratio of the activated carbon and wood fiber is 2 ~5:1;

[0028] (5) Sterilize after filtering the semi-decolorized collagen peptide to obtain steril...

Embodiment 1

[0042] Add tap water to the scales of tilapia according to the mass ratio of substrate 1:5, and wash them three times in a stirring tank for 20 minutes each time.

[0043] Add tap water to the cleaned fish scales according to the mass ratio of substrate 1:8, raise the temperature to 30-60°C, add 0.15-1.5% lipase for lipidation, and stir for 30 minutes with an enzyme activity of 20,000 U / g; heat up to 90°C in the tank Stay in the middle for 10min, drain the liquid.

[0044] After the liquid is discharged, add pure water again according to the mass ratio of substrate 1:10, pour into the tank and stir to enter the wall breaking treatment, but the liquid raw material is crushed to 30-60 mesh.

[0045] The broken raw material is added with a compound protease with an enzyme activity of 30,000 U / g according to 0.1-0.5% of the substrate, enzymatically hydrolyzed at 50-55°C for 4 hours, heated to 90°C to inactivate the enzyme for 10 minutes, coarsely filtered, and centrifuged.

[004...

Embodiment 2

[0051] Put the tilapia fish scales into the container and wash with tap water three times under agitation and drain to remove impurities.

[0052] Pour the cleaned fish scales into tap water, raise the temperature to 30-60°C according to the mass ratio of substrate 1:8, add 0.15-1.5% lipase for lipidation, and stir for 20 minutes with an enzyme activity of 20,000 U / g, then heat up to 90°C again to kill the enzyme Drain for 10 minutes.

[0053] Inject the enzyme-inactivated fish scale collagen peptide into pure water at a mass ratio of 1:8, stir, and inject into crushing equipment to obtain a solid-liquid liquid of 20-40 mesh.

[0054] Put the solid-liquid liquid into the enzymolysis tank, raise the temperature to 50-60°C, add compound protease according to the substrate 2%-3% enzyme activity 30,000 U / g, stir for 3.5 hours, pass through a 5-hole filter, 80-mesh filter bag, Centrifuge.

[0055] Pour the centrifuged fish scale collagen peptide into the decolorization tank, keep...

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Abstract

The invention provides a preparation method of a fish scale collagen peptide, and relates to the technical field of fish scale collagen peptide preparation. The preparation method of the fish scale collagen peptide comprises the steps that fish scales are subjected to being cleaned, lipidation, wall breaking, enzymatic hydrolysis, rough filtration, centrifugation, decoloration, sterilization, refined filtration, concentration and drying, the fish scale collagen peptide is prepared. According to the preparation method of the fish scale collagen peptide, a physical method is adopted, cleaning and warming are carried out, added enzymatic lipidation is carried out, the fish scales are subjected to wall breaking, the structure of the fish scales is broken, the contact area of compound proteaseand materials is convenient to increase, and proteins are effectively extracted and the magnitude of shear molecular weight is effectively controlled; and meanwhile, the whole process takes 6-7 hours,and time is shortened and the labor cost is saved; and during decoloration, activated carbon is blended with wood fiber, the fish scale collagen peptide is filtered, and the environmental requirements are facilitated. Environmental protection and reduced energy and consumption are achieved in the whole preparation method, and meanwhile, the quality and the proteion extraction content of the fishscale collagen peptide are further improved.

Description

technical field [0001] The invention belongs to the technical field of fish scale collagen peptide preparation, and in particular relates to a fish scale collagen peptide and a preparation method thereof. Background technique [0002] Collagen protein peptide is an important low-molecular structure of functional protein, which is closely related to cell proliferation, differentiation, movement, immunity, joint lubrication, wound healing and so on. Due to the special functions of low-molecular-weight protein peptides, its extracts have long been widely used in the fields of medical treatment, food, daily chemical industry, and biosynthesis, such as: medical surgical materials, functional drinks, food cakes, cosmetics, etc. [0003] In the past, the main source of raw materials for the production of collagen peptides was pigs, cattle and other terrestrial mammals. This year, changes in the global ecological environment have led to many mad cow disease, foot-and-mouth disease, ...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34C07K14/78
CPCC07K14/78C12P21/06
Inventor 黄荣涛林文浩王瑞涛
Owner 汕尾市维明生物科技有限公司
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