Probiotic preparation used for improving growth performance, immune performance and nutritional quality of scophthalmus maximus

A probiotic preparation and immune performance technology, applied in the field of microecological preparations, can solve the problems affecting the sustainable development of turbot aquaculture, eutrophication of water bodies, degradation of seedlings, etc. The effect of improving growth performance

Pending Publication Date: 2020-01-07
DALIAN NATIONALITIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the continuous expansion of this high-density, intensive, and industrialized farming scale, a series of problems have followed, such as eutrophication of water bodies, poor water quality, degradation of seedlings, and aggravation of diseases, etc., which seriously affect turbot. Sustai

Method used

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  • Probiotic preparation used for improving growth performance, immune performance and nutritional quality of scophthalmus maximus
  • Probiotic preparation used for improving growth performance, immune performance and nutritional quality of scophthalmus maximus
  • Probiotic preparation used for improving growth performance, immune performance and nutritional quality of scophthalmus maximus

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0031] Example 1

[0032] (1) Activated strain

[0033] Prepare LB solid medium, make slopes and plates, take out the glycerol tube of the experimental strain Bacillus simum in the refrigerator at -80℃, thawed naturally, use bamboo skewers to mark the slope, cultivate at 30℃, after 3~7d cultivation , Kept in a refrigerator at 4℃ for later use.

[0034] (2) Multiplication of strains

[0035] Pick up the activated experimental strains with bamboo sticks, inoculate them in LB liquid medium according to the inoculation amount of 8%, and cultivate them on a constant temperature shaker at 30°C for 48 hours to obtain Bacillus simmons liquid;

[0036] (3) Preparation of bacterial suspension

[0037] Centrifugally concentrate the Bacillus simum solution at 6000r / min for 10min, configure it into a uniformly distributed bacterial suspension, and count the live bacteria under the microscope to make the number of live bacteria ≧10 10 cfu / mL.

[0038] (4) Preparation of feed

[0039] Add the bacterial ...

Example Embodiment

[0044] Example 2 Effect of probiotic preparations on the growth performance of turbot

[0045] Table 1 Growth performance of turbot

[0046]

[0047] Note: Different lowercase letters in the same industry indicate significant differences (P <0.05)

[0048] The effect of adding Bacillus simum in feed on the growth performance of turbot is shown in Table 1. It can be seen from the table that after the feeding experiment, the addition of Bacillus simum to the feed has a significant effect on the growth of turbot. The specific results are as follows: Compared with the control group (T0), the final body weight and body weight gain rate of the treatment group (T) with Bacillus simum addition to the feed increased significantly (P <0.05), the bait coefficient is also significantly reduced. It shows that adding Bacillus simum to feed can improve the growth performance of turbot and increase the utilization rate of bait.

Example Embodiment

[0049] Example 3 Effect of probiotic preparation on turbot shape index

[0050] Table 2 Physical indicators of turbot

[0051]

[0052] Note: Different lowercase letters in the same industry indicate significant differences (P <0.05)

[0053] See Table 2 for the effect of adding Bacillus simum in feed on turbot shape index. It can be seen from Table 2 that compared with T0, the plumpness of T1 is significantly increased. However, there was no significant difference in the ratio of viscera to body, liver to body ratio and intestinal body ratio, indicating that the addition of Bacillus simmons significantly improved the plumpness of turbot, without adversely affecting its health.

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Abstract

The invention belongs to the field of micro-ecology preparations, and particularly relates to a probiotic preparation used for improving the growth performance, the immune performance and the nutritional quality of scophthalmus maximus. Bacillus siamensis is added into scophthalmus maximus basal feed at a certain ratio for granulation, wherein a viable count in the feed is greater than or equal to10<9>cfu/g. The scophthalmus maximus is fed for eight weeks, and is fed twice a day, wherein a feeding amount is 1.5% of body weight; water is changed twice a day, wherein a water changing amount is40-60% of a total volume; and after feeding is finished, the probiotic preparation has an effect of obviously improving the growth performance, the immune performance and the nutritional quality of scophthalmus maximus. By use of the invention, the growth performance and the nutritive value of the scophthalmus maximus are effectively improved, a bait use ratio is improved, meanwhile, the immunocompetence of a fish body can be enhanced, the scophthalmus maximus can healthily grow, diseases are reduced, and a higher economic value is created.

Description

technical field [0001] The invention belongs to the field of microecological preparations, in particular to a probiotic preparation for improving the growth performance, immune performance and nutritional quality of turbot. Background technique [0002] Turbot is an internationally recognized high-value farmed fish. It was first introduced into China by the Yellow Sea Fisheries Research Institute of the Chinese Academy of Fishery Sciences in 1992. After years of scientific research, breeding and promotion, turbot has realized industrial farming. However, with the continuous expansion of this high-density, intensive, and industrialized farming scale, a series of problems have followed, such as eutrophication of water bodies, poor water quality, degradation of seedlings, and aggravation of diseases, etc., which seriously affect turbot. sustainable development of farming. In order to prevent and control diseases, farmers first consider the use of antibiotics, but food safety i...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K50/80C12N1/20C12R1/07
CPCA23K10/18A23K50/80C12N1/20Y02A40/81
Inventor 于基成张美娇刘秋覃志慧
Owner DALIAN NATIONALITIES UNIVERSITY
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