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Probiotic preparation used for improving growth performance, immune performance and nutritional quality of scophthalmus maximus

A probiotic preparation and immune performance technology, applied in the field of microecological preparations, can solve the problems affecting the sustainable development of turbot aquaculture, eutrophication of water bodies, degradation of seedlings, etc. The effect of improving growth performance

Pending Publication Date: 2020-01-07
DALIAN NATIONALITIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the continuous expansion of this high-density, intensive, and industrialized farming scale, a series of problems have followed, such as water eutrophication, poor water quality, seedling degradation, and disease aggravation, which seriously affect turbot. Sustainable Development of Farming Industry
In order to prevent and control diseases, farmers first consider the use of antibiotics, but food safety issues such as drug resistance and drug residues caused by improper use of antibiotics have attracted people's attention. , sustainable development is crucial

Method used

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  • Probiotic preparation used for improving growth performance, immune performance and nutritional quality of scophthalmus maximus
  • Probiotic preparation used for improving growth performance, immune performance and nutritional quality of scophthalmus maximus
  • Probiotic preparation used for improving growth performance, immune performance and nutritional quality of scophthalmus maximus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Activated strain

[0033] Prepare LB solid medium, make it into a slant and a flat plate, take out the glycerol tube of the experimental strain Bacillus Simim in a -80°C refrigerator and thaw it naturally, draw a line on the slant with a bamboo stick, and cultivate at 30°C. After 3-7 days of cultivation , were stored in a 4°C refrigerator for later use.

[0034] (2) Propagation of strains

[0035] Pick the activated experimental strain with a bamboo stick, inoculate it in LB liquid medium according to the inoculum size of 8%, and cultivate it on a constant temperature shaker at 30°C for 48 hours to obtain a Sime bacillus liquid;

[0036] (3) Preparation of bacterial suspension

[0037] Concentrate the Bacillus spp. liquid by centrifugation at 6000r / min for 10min, configure it into a uniformly distributed bacterial suspension, and count the viable bacteria under a microscope to make the number of viable bacteria ≧10 10 cfu / mL.

[0038] (4) Feed preparation

[00...

Embodiment 2

[0044] The influence of embodiment 2 probiotics preparation on turbot growth performance

[0045] Table 1 Growth performance of turbot

[0046]

[0047] Note: Different lowercase letters in the same row indicate significant difference (P<0.05)

[0048] See Table 1 for the effects of adding Bacillus Simem to the feed on the growth performance of turbot. It can be seen from the table that after the feeding experiment, the addition of Bacillus Simem to the feed had a significant effect on the growth of turbot. The specific results are as follows: Compared with the control group (T0), the final body weight and body weight gain rate of the treatment group (T) added with Bacillus simum in the feed were significantly increased (P<0.05), and the feed coefficient was also significantly reduced. . It indicated that adding Bacillus simum to feed could improve the growth performance of turbot and improve the utilization rate of bait.

Embodiment 3

[0049] The influence of embodiment 3 probiotics preparation on turbot body index

[0050] Table 2 Physical indicators of turbot

[0051]

[0052] Note: Different lowercase letters in the same row indicate significant difference (P<0.05)

[0053] See Table 2 for the effects of adding Bacillus simum in the feed on the shape indicators of turbot. It can be seen from Table 2 that compared with T0, the plumpness of T1 was significantly increased. However, there were no significant differences in viscera-to-body ratio, liver-to-body ratio, and intestine-to-body ratio, which indicated that the addition of Bacillus Simem had significantly improved the plumpness of turbot, and had no adverse effects on its health.

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Abstract

The invention belongs to the field of micro-ecology preparations, and particularly relates to a probiotic preparation used for improving the growth performance, the immune performance and the nutritional quality of scophthalmus maximus. Bacillus siamensis is added into scophthalmus maximus basal feed at a certain ratio for granulation, wherein a viable count in the feed is greater than or equal to10<9>cfu / g. The scophthalmus maximus is fed for eight weeks, and is fed twice a day, wherein a feeding amount is 1.5% of body weight; water is changed twice a day, wherein a water changing amount is40-60% of a total volume; and after feeding is finished, the probiotic preparation has an effect of obviously improving the growth performance, the immune performance and the nutritional quality of scophthalmus maximus. By use of the invention, the growth performance and the nutritive value of the scophthalmus maximus are effectively improved, a bait use ratio is improved, meanwhile, the immunocompetence of a fish body can be enhanced, the scophthalmus maximus can healthily grow, diseases are reduced, and a higher economic value is created.

Description

technical field [0001] The invention belongs to the field of microecological preparations, in particular to a probiotic preparation for improving the growth performance, immune performance and nutritional quality of turbot. Background technique [0002] Turbot is an internationally recognized high-value farmed fish. It was first introduced into China by the Yellow Sea Fisheries Research Institute of the Chinese Academy of Fishery Sciences in 1992. After years of scientific research, breeding and promotion, turbot has realized industrial farming. However, with the continuous expansion of this high-density, intensive, and industrialized farming scale, a series of problems have followed, such as eutrophication of water bodies, poor water quality, degradation of seedlings, and aggravation of diseases, etc., which seriously affect turbot. sustainable development of farming. In order to prevent and control diseases, farmers first consider the use of antibiotics, but food safety i...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K50/80C12N1/20C12R1/07
CPCA23K10/18A23K50/80C12N1/20Y02A40/81
Inventor 于基成张美娇刘秋覃志慧
Owner DALIAN NATIONALITIES UNIVERSITY
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