Application of xylocarpin H and analogues thereof in preparation of neuroprotective medicines or foods
A kind of technology of neem and its analogs, applied in the field of medicine
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Embodiment 1
[0041] Example 1 Preparation of Nibabin H
[0042] Neem H is a monomer isolated and extracted from Neem, with a purity of more than 98% through identification.
[0043] Method for Extraction, Separation and Purification of Nibatin H
[0044] Take about 20Kg of crushed neem medicinal material (fruit or seeds), cold-soak and extract with 95% ethanol, and extract three times for one week each time. The extract was filtered and concentrated under reduced pressure to a paste to obtain an extract. Suspend the extract in water, extract with petroleum ether, dichloromethane and ethyl acetate respectively, and concentrate the extract under reduced pressure to obtain partial extracts of petroleum ether, partial extracts of dichloromethane and partial extracts of ethyl acetate. The chemical components of the above parts were separated by silica gel column chromatography, dextran gel, preparative thin-layer method and preparative high-performance liquid chromatography, and the obtaine...
experiment example 1
[0047] Experimental example 1 Effects on CORT-induced neuroblastoma cell SH-SY5Y injury
[0048] Neuroblastoma cells SH-SY5Y were cultured in DMEM medium containing 15% fetal bovine serum at 37°C and 5% CO2, digested and passaged with 0.1% trypsin. Neuroblastoma cells SH-SY5Y (2×10 5 cells / mL) were inoculated in 96-well cell culture plates, and used for experiments after adhering to the wall.
[0049] Cells were divided into blank group, blank control group, model group, positive control group and drug administration group.
[0050] Add 100 μL DMEM without adding cells to the blank group to correct the optical density value, add 100 μL DMEM to the blank control group, add 100 μL DMEM containing 100 μmol / L corticosterone (Cort) to the model group; At the same time, add oxalic acid containing 12μmol / L- S - Citalopram (ESC), test compound xylocarpin H 1, 4, 8, 16, 64 μmol / L. After 48 hours, add 10 μL CCK-8 solution to each well, place the culture plate in an incubator and i...
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