Pseudomonas putida S9 and application thereof in promotion of coprinus comatus growth

A technology of Pseudomonas putida and Coprinus comatus, applied in the direction of application, microbe-based methods, bacteria, etc., can solve the problems of high chemical pollution and low yield, and achieve the effects of increasing growth rate, accelerating growth rate, and increasing yield

Active Publication Date: 2020-01-10
QUFU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] For the deficiencies in the prior art, the technical problem to be solved by this invention is to provide a kind of Pseudomonas putida S9, which has a promoting effect on the growth of Coprinus comatus; the technical problem to be solved by this invention is to provide Pseudomonas putida S9 in Promote the application of Coprinus comatus in the growth, in order to solve the problem of low yield and high chemical pollution of traditional Coprinus comatus planting method

Method used

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  • Pseudomonas putida S9 and application thereof in promotion of coprinus comatus growth
  • Pseudomonas putida S9 and application thereof in promotion of coprinus comatus growth
  • Pseudomonas putida S9 and application thereof in promotion of coprinus comatus growth

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Embodiment 1

[0020] 1) Isolation, screening and identification of bacteria

[0021] The cultivation material of Coprinus comatus was randomly collected from Jinxiang County Chenyu Bacteria Industry Development Co., Ltd., and 42 bacterial isolates were isolated from CCP by dilution and coating on enriched medium enrichment plates. Then use NA medium for separation and purification.

[0022] Among them, the formula of NA medium is peptone 10g, beef extract 5g, sodium chloride 5g, agar 20g, pH7.2~7.4.

[0023] Put the strains preserved after primary screening, isolation and purification into conical flasks of NA liquid medium, and culture them on a shaker at 28°C and 180r / min for 48 hours to obtain saturated bacterial liquids, and perform aseptic operation with a pipette gun It is required to inoculate 0.2mL of each bacterial solution on enriched medium plate (glucose 20g, potato 200g, peptone 10g, yeast extract 3g, agar 15-20g, water 1000mL, pH7.0) and spread evenly with a spreader. The ho...

Embodiment 2

[0030] Effect of Pseudomonas putida S9 on fruiting

[0031] In the workshop of the school’s cooperative edible fungus enterprise, a planting area of ​​200 square meters was selected. The growth environment, ingredient formula, fermentation, and bagging were consistent with standardized production. Before inoculation of 100 square meters of covered soil, the concentration per square meter was 1.5× 10 9 cfu / mL~2.0×10 9 Cfu / mL of Pseudomonas putida S9 bacterial solution 500mL, 100 square meters of soil covered with the same total volume of sterile water as a control, observe the fruiting speed and output of Coprinus comatus. Effects of Pseudomonas putida S9 on fruiting body characters of Coprinus comatus strains as follows Figure 3 to Figure 5 as shown, image 3 It shows that the average stipe height of Coprinus comatus is 2cm higher than the average stipe height of Coprinus comatus; Figure 4 showed a 10% increase in the biotransformation rate of Coprinus comatus added with...

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Abstract

The invention discloses pseudomonas putida S9 and application thereof in promotion of coprinus comatus growth and relates to the biotechnical field of edible mushrooms. The pseudomonas putida is classified and named pseudomonas putida S9, is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC No:M 2019635, the preservation date of August 16, 2019 and thepreservation address of Wuhan University, Wuhan city, China. When the pseudomonas putida S9 is applied to promotion of coprinus comatus growth, the coprinus comatus added with the pseudomonas putidaS9 has a high growing speed, thick hyphae, long sporocarp stipe and increasing sporocarp yield, the growing speed of coprinus comatus can be effectively accelerated, the production cycle of the coprinus comatus can be shortened, the yield of coprinus comatus can be increased, the product is ensured to be environment-friendly, healthy and pollution-free, auxiliary dosage can be reduced, and the production economic and social benefits of edible mushrooms can be increased.

Description

technical field [0001] The invention relates to the field of edible fungus biotechnology, in particular to Pseudomonas putida S9 and its application in promoting the growth of Coprinus comatus. Background technique [0002] Coprinus comatus, the scientific name is Coprinus coprinus, is named for its shape like a chicken leg, and its meat is like shredded chicken. It has no chicken smell. It is a rare fungus with commercial potential developed artificially in recent years. It is known as "the new star of fungi ". Coprinus comatus is rich in nutrition, delicious in taste, excellent in taste, and has high nutritional value. The fruiting body is larger, and the cap is cylindrical. When the umbrella is opened, the edge gills melt into an ink-like liquid within 40 minutes, and the stipe becomes slender at the same time. The diameter of the cap is 3-5cm, the height is 9-11cm, and the surface is brown to light brown. As the cap grows, it breaks into larger scales. The flesh is wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01G18/00C12R1/40
CPCA01G18/00C12R2001/40C12N1/205
Inventor 李冠喜李秋月于颖媛张璐张鹤露亓超凡吴克兢郭帅
Owner QUFU NORMAL UNIV
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