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Cationic antibacterial peptide modified by dopamine or derivative thereof as well as preparation and application thereof

An antimicrobial peptide and dopamine technology, which is applied in the field of antibacterial biomaterials, can solve the problems of human cytotoxicity and instability of natural antimicrobial peptide protease, etc., and achieve good adhesion performance, high-efficiency broad-spectrum killing effect, good adhesion performance and broad-spectrum The effect of antibacterial function

Active Publication Date: 2020-01-14
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above defects or improvement needs of the prior art, the present invention provides a cationic antimicrobial peptide modified by dopamine or its derivatives, its preparation and application, which is a cationic antimicrobial peptide modified by dopamine adhesion group, including hydrophilic The cationic amino acid and the anionic amino acid and hydrophobic amino acid modified by the side chain of dopamine, thereby solving the instability of the natural antimicrobial peptide protease in the prior art, and although the synthetic cationic antimicrobial peptide has strong antibacterial activity, it is harmful to human cells. There are also some technical problems such as toxicity

Method used

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  • Cationic antibacterial peptide modified by dopamine or derivative thereof as well as preparation and application thereof
  • Cationic antibacterial peptide modified by dopamine or derivative thereof as well as preparation and application thereof
  • Cationic antibacterial peptide modified by dopamine or derivative thereof as well as preparation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0042] The amino acid sequence of the cationic antibacterial peptide is KKFEFEFEFEKK, the structural formula of the antibacterial peptide is KKFE(DA)FE(DA)FE(DA)FE(DA)KK-NH2, and DA is dopamine.

[0043] Note: The C-terminus in the structural formula of the antimicrobial peptide contains -NH2. Those skilled in the art can understand that the carboxyl amidation of the antimicrobial peptide belongs to the conventional treatment method of the antimicrobial peptide. E(DA) indicates that dopamine is modified on the side chain of glutamic acid through an amide bond.

[0044] The preparation method of above-mentioned antimicrobial peptide:

[0045] (1) Weigh 0.3 g of Rink Amide-AM resin to the peptide synthesis device, add dry N,N-dimethylformamide (DMF) and shake for 2 hours to swell the resin. Then, 10 mL of DMF solution containing 20% ​​piperidine (volume fraction) was used to remove the protective group of the resin, and the operation was repeated twice, each lasting 20 minutes....

Embodiment 13 to Embodiment 17

[0056] From Example 13 to Example 17, except that dopamine is replaced by one of α-methyldopamine, norepinephrine, levodopa, α-methyldopa and droxidopa, the parameters used, Condition etc. all keep consistent with embodiment 1.

Embodiment 18

[0058] The antimicrobial peptide prepared in Example 1 was formulated into an aqueous solution with a mass fraction of 2%, and the pH value was adjusted to 2.5 with dilute hydrochloric acid. After 6 hours of irradiation under a 254nm ultraviolet lamp, the above solution undergoes a sol-gel transition to obtain an antimicrobial peptide hydrogel.

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Abstract

The invention belongs to the field of antibacterial biological materials, and particularly relates to a cationic antibacterial peptide modified by dopamine or derivatives thereof as well as a preparation and an application thereof. The amino acid sequence of the antibacterial peptide from the N terminal to the C terminal is XX (AB) nXX, n is an integer from 3 to 8, X is a cationic amino acid, A isa hydrophobic amino acid, and B is an anionic amino acid. The antibacterial peptide containing a dopamine adhesion group shows a broad spectrum and excellent antibacterial performance. The antibacterial peptide containing the dopamine adhesion group provided by the invention has the property of forming hydrogel through ultraviolet irradiation. In addition, the antibacterial peptide containing thedopamine adhesion group, provided by the invention, can be used for tissue damage repair and antibacterial materials, and has good application prospect and value.

Description

technical field [0001] The invention belongs to the field of antibacterial biomaterials, and more specifically relates to a cationic antibacterial peptide modified by dopamine or its derivatives, its preparation and application. Background technique [0002] In recent years, the overuse or abuse of antibiotics has led to the emergence of multidrug-resistant strains, which are resistant to multiple or even all antibacterial drugs, that is, "super bacteria". With the emergence of multi-antibiotic-resistant bacteria, it is becoming more and more difficult to treat bacterial infections with existing antibiotics, and there is an urgent need to find new ways to solve this problem. At present, in order to deal with the infections caused by drug-resistant bacteria, on the one hand, people have modified the structure of traditional antibiotics to improve the curative effect while reducing bacterial drug resistance, and on the other hand, they are constantly developing new antibacteri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C07K1/107C07K1/04A61K9/06A61P17/02A61P31/04
CPCA61K9/06A61K38/00A61P17/02A61P31/04C07K7/08
Inventor 朱锦涛穆巴沙·侯赛因蒋皓陶娟谢盈
Owner HUAZHONG UNIV OF SCI & TECH
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