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Method for preparing cross-linked dextran gels resistant to alpha-glucosidase hydrolysis

A technology of dextran gel and glucosidase, applied in the field of materials, can solve problems such as adverse reactions, and achieve the effects of reducing catalytic efficiency, enhancing maintenance time and plastic effects, and large economic benefits

Active Publication Date: 2022-07-12
宁夏妙朗生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, to improve the maintenance time of polysaccharide hydrogel, only the mode of increasing the degree of chemical cross-linking is used, but the increase of the content of chemical cross-linking agent may lead to subsequent adverse reactions

Method used

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  • Method for preparing cross-linked dextran gels resistant to alpha-glucosidase hydrolysis
  • Method for preparing cross-linked dextran gels resistant to alpha-glucosidase hydrolysis
  • Method for preparing cross-linked dextran gels resistant to alpha-glucosidase hydrolysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Low molecular weight dextran at an activation concentration of 20% (w / v) becomes a biocrosslinking intermediate:

[0027] Take 20% (w / v) low molecular weight dextran (10000-20000Da) solution in a 15mL centrifuge tube, according to the molar ratio of low molecular weight dextran functional monomer and epoxy group-containing activator to 1:1 , 1:2, 1:4, 1:6; 1:8; 1:10, add 1,4-butanediol diglycidyl ether (BDDE), ethylene glycol diglycidyl ether (EGDE), Oxychloropropane or the like is used as an activator for the activation reaction. The reaction system contains 0.3M NaOH as a catalyst, and reacts at 30°C for 4 hours to obtain a mild and effective biological cross-linking intermediate, that is, epoxy-activated low-molecular-weight dextran. It was precipitated with 75% ethanol and washed 3 times and lyophilized to powder. After dissolving it in pure water, the density of epoxy groups contained in the biocrosslinked intermediate was measured by a sodium thiosulfate titrati...

Embodiment 2

[0034] Activation of low molecular weight dextran at different concentrations into biocrosslinking intermediates:

[0035] Take low molecular weight dextran (10000-20000Da) solutions with concentrations of 5%, 10%, 15%, 20%, 25% and 30% (w / v) respectively in a 15mL centrifuge tube. The molar ratio of glycoside functional monomer and activator is 1:4, and 1,4-butanediol diglycidyl ether (BDDE), ethylene glycol diglycidyl ether (EGDE), epichlorohydrin, etc. are added as activators respectively. Activation reaction is carried out. The reaction system contains 0.3M NaOH as a catalyst, and the reaction is carried out at 30° C. for 4 hours to obtain a biologically cross-linked intermediate containing an epoxy group. The cells were precipitated with 75% ethanol and washed 3 times before lyophilization to powder. After dissolving in pure water, the density of epoxy groups contained in the biocrosslinked intermediate was measured by sodium thiosulfate titration. When the concentrati...

Embodiment 3

[0039] Example 3 The influence of the molar ratio of the epoxy group contained in the biological cross-linking intermediate to the high-molecular-weight dextran functional monomer on the dextrorotatory content in the gel after cross-linking:

[0040]Take 30% (w / v) high molecular weight dextran (700000-1000000Da) solution in a 15mL centrifuge tube, according to the molar ratio of the epoxy group contained in the biological cross-linking intermediate to the high molecular weight dextran functional monomer For the ratio of 1:10, 1:20, 1:30, 1:40, 1:15, 1:60, 1:80 and 1:100, add 1,4-butanediol diglycidyl ether ( BDDE), ethylene glycol diglycidyl ether (EGDE), and epichlorohydrin as activators. The reaction system contained 0.2M NaOH as a catalyst, and the reaction was carried out at 30°C for 4 hours. Prepared into gel particles. The particles were completely vacuum lyophilized and the content of dextran per gram of gel was calculated. When the molar ratio of the epoxy group con...

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Abstract

The present invention provides a method for preparing a cross-linked dextran gel resistant to hydrolysis by α-glucosidase. The low-molecular-weight dextran is used as the starting skeleton, and an activator containing an epoxy group is used to activate it to make It has a plurality of epoxy groups, activated low-molecular-weight dextran; the epoxy-containing activator is 1,4-butanediol diglycidyl ether, ethylene glycol diglycidyl ether or epoxy A kind of chloropropane; using activated low molecular weight dextran as a biological cross-linking intermediate to mix with high molecular weight dextran and carry out a cross-linking reaction to form a hydrogel, and homogenize to form uniform gel particles; adding N -Acetyl-D-glucosamine, D-glucosamine, and cellobiose functional monomers. In the gel prepared by the method, the catalytic efficiency of α-glucosidase is reduced by more than 50% in the experiment of in vitro dextran hydrolysis.

Description

technical field [0001] The invention relates to a method for preparing a cross-linked dextran gel resistant to hydrolysis by alpha-glucosidase, and belongs to the field of materials. Background technique [0002] Dextran is a kind of polymeric polysaccharide with α-glycosidic bond as link and glucose as the basic functional monomer. Because it has no antigenicity and can be structurally stable, and can withstand high temperature and autoclave sterilization, it can be used as a plasma expander. . Dextran hydrogel has good water retention properties and high stability, and has great application potential in the field of injection filling in the plastic and cosmetic industry. Dextran has been shown to be effective in enhancing the body's immune system and resisting diseases caused by microorganisms. In addition, dextran still has the functions of scavenging free radicals, resisting radiation, dissolving cholesterol, preventing hyperlipidemia, and resisting infections caused b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08J3/24C08L5/02
CPCC08J3/075C08J3/246C08J2305/02C08J2405/02
Inventor 辛瑜
Owner 宁夏妙朗生物科技有限公司
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