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A kind of preparation method of high-density yeast fermentation broth

A yeast fermentation, high-density technology, applied in fungi and other directions, can solve the problems of unfavorable industrial development, high production cost, and high comprehensiveness, and achieve the effect of reducing sulfate ion content, less production water, and small dosage

Active Publication Date: 2022-05-06
FLSUGARPEPTIDE BIOLOGY ENG
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The traditional yeast value-added fermentation fermentation method achieves high-density fermentation of Saccharomyces cerevisiae by optimizing the culture conditions, and integrates and optimizes many factors and their interactions to form a complete fermentation process. This process is a complex and comprehensive work. , not conducive to the needs of industrial development
In addition, yeast culture mostly uses raw materials such as glucose and yeast extract as reducing sugar and nitrogen sources, and the production cost is high

Method used

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  • A kind of preparation method of high-density yeast fermentation broth

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Effect test

Embodiment 1

[0039] A preparation method of high-density yeast fermentation broth, the specific operation method is as follows:

[0040] (1), preparation of dilute compound nutrient solution and concentrated compound nutrient solution:

[0041] Crush 95kg of corn and 5kg of soybean meal, add 200kg of water to make a slurry, adjust the pH to 6.0, add 0.04kg of high-temperature amylase, and enzymatically hydrolyze at 90°C for 1 hour; cool down to 55°C, adjust the pH to 5.2, and add 0.04kg of saccharification Enzyme, keep warm for 5 hours; adjust the pH value to 4.8, add 0.06kg of protease, keep warm for 15 hours to make a liquid enzymatic solution;

[0042] Filtrate the liquid enzymatic solution to obtain 260 kg of filtrate, which is the dilute compound nutrient solution. After the dilute compound nutrient solution is evaporated and concentrated under reduced pressure, 163 kg of concentrated compound nutrient solution is obtained, with a solid content of 44%.

[0043] (2), the production me...

Embodiment 2

[0047] A preparation method of high-density yeast fermentation broth, the specific method is as follows:

[0048] (1), preparation of dilute compound nutrient solution and concentrated compound nutrient solution:

[0049] Grind 1850kg of corn and 150kg of soybean meal, add 4400kg of water to make a slurry, adjust the pH value to 6.0, add 0.8kg of high-temperature amylase, enzymatic hydrolysis at 90°C for 1 hour; cool down to 55°C, adjust the pH value to 5.2, add 0.8 kg of glucoamylase, heat preservation enzymolysis for 5 hours; adjust the pH value to 4.8, add 1.2kg of protease, heat preservation enzymolysis time 15 hours; obtain the filtrate 5416kg through filtration, which is the dilute compound nutrient solution. The concentrated compound nutrient solution is 3520kg, and the solid content is 40%.

[0050] (2), the production method of high-density yeast liquid is as follows:

[0051] ①. In a 2m3 fermentation tank, dilute 25kg of dilute compound nutrient solution to 0.9m3, ...

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Abstract

The invention relates to a preparation method of a high-density yeast fermentation liquid. The corn soybean meal is mixed with water, and high-temperature amylase, glucoamylase, and acidic protein are respectively added to enzymolyze thoroughly, and the solid and liquid are separated to obtain a solid wet residue and a dilute compound nutrient solution. . Part of the dilute compound nutrient solution is diluted and inserted into the yeast seed solution for cultivation, and the remaining dilute compound nutrient solution is evaporated under reduced pressure to obtain a concentrated solution. During the cultivation process, sufficient dissolved oxygen is controlled by adding concentrated compound nutrient solution and nutrient salt. When the yeast density is at its maximum, the feeding is stopped. After the fermentable sugar is exhausted, the yeast fermentation liquid is obtained, and the high density is obtained by standing still. Yeast liquid. The present invention enzymolyzes different enzyme preparations into sugars and amino acids needed for yeast growth, and obtains high-density yeast liquid through continuous fed-batch fermentation and batch discharge. Less, reduce the discharge of wastewater from fermentation tank washing, and achieve the effects of energy saving, consumption reduction and clean production.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a preparation method of high-density yeast fermentation liquid. Background technique [0002] The nutritional value of Saccharomyces cerevisiae protein is very high, the protein content in yeast dry matter can be as high as 50%, the essential amino acid content required by animals is high, close to animal protein, and the net protein utilization rate is much higher than plant protein. In addition, Saccharomyces cerevisiae is also rich in nucleotides, B vitamins, minerals and other physiologically active substances. With the development of microbial engineering and fermentation engineering, Saccharomyces cerevisiae is more and more widely used in the production of high-quality protein feed. [0003] At the same time, with the wide application of yeast in feed, realizing high-density fermentation of yeast in a limited fermenter space is the key to mainta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16
CPCC12N1/16
Inventor 符秀迪程勐万里孟龙薛芹
Owner FLSUGARPEPTIDE BIOLOGY ENG
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