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Preparation method of micro-well structure SPRi chip and product and application thereof

A micro-well and chip technology, which is applied in the field of micro-well structure SPRi chip preparation, can solve the problems of complicated Jindao array chip processing procedures, limited detection throughput, and high cost, and achieve high-efficiency SPRi multi-component detection and parallel analysis, Easy extraction and analysis, high contrast effect

Active Publication Date: 2020-01-17
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the two methods have their own disadvantages: the gold island array chip in the first method has the disadvantages of complex processing procedures, high cost, and limited detection throughput; while the ordinary continuous gold film chip in the second method has the disadvantages It has the advantages of low and high detection throughput, but there are problems such as unclear boundaries between the sensing area and the background area, large noise in the background area, and low contrast of the obtained SPRi photos, which lead to the decline of SPRi detection performance

Method used

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  • Preparation method of micro-well structure SPRi chip and product and application thereof
  • Preparation method of micro-well structure SPRi chip and product and application thereof
  • Preparation method of micro-well structure SPRi chip and product and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Prepare the microwell structure SPRi chip, the preparation process is as follows figure 1 As shown, the preparation method is as follows:

[0043] (1) choose the continuous flat gold film composition chip that is covered in the thickness 45nm of optical glass surface to be the gold film chip;

[0044] (2) Immerse the above-mentioned clean gold film chip in trihydroxy-methylaminomethane hydrochloride buffer solution containing polydopamine and pH=8.5 (wherein the volume mass concentration of polydopamine is 2 mg / mL), and take out the chip from the solution , washed with secondary water, blown dry with nitrogen;

[0045] (3) repeat the operation of step (2) with the gold film that above-mentioned nitrogen blows dry, make the polydopamine film thickness on the gold film chip reach 15nm, obtain the continuous gold film chip of polydopamine modification;

[0046](4) Place a photomask on the prepared polydopamine-modified continuous gold film chip, in the ultraviolet cleani...

Embodiment 2

[0048] Prepare microwell structure SPRi chip, its preparation method is as follows:

[0049] (1) choose to have the continuous smooth gold film composition chip that is covered in the thickness 50nm of optical glass surface to be gold film chip;

[0050] (2) Immerse the above-mentioned clean gold film chip in trihydroxy-methylaminomethane hydrochloride buffer solution containing polydopamine and pH=8.5 (wherein the volume mass concentration of polydopamine is 2 mg / mL), and take out the chip from the solution , washed with secondary water, blown dry with nitrogen;

[0051] (3) repeat the operation of step (2) with the gold film that above-mentioned nitrogen blows dry, make the polydopamine film thickness on the gold film chip reach 100nm, obtain the continuous gold film chip of polydopamine modification;

[0052] (4) Place a photomask on the prepared polydopamine-modified continuous gold film chip, in the ultraviolet cleaning instrument with Hg-Xel light source, set power 110W...

Embodiment 3

[0054] Prepare microwell structure SPRi chip, its preparation method is as follows:

[0055] (1) choose the continuous flat gold film composition chip that is covered in the thickness 55nm of optical glass surface to be the gold film chip;

[0056] (2) Immerse the above-mentioned clean gold film chip in trihydroxy-methylaminomethane hydrochloride buffer solution containing polydopamine and pH=8.5 (wherein the volume mass concentration of polydopamine is 2 mg / mL), and take out the chip from the solution , washed with secondary water, blown dry with nitrogen;

[0057] (3) repeat the operation of step (2) with the gold film that above-mentioned nitrogen blows dry, make the polydopamine film thickness on the gold film chip reach 60nm, obtain the continuous gold film chip of polydopamine modification;

[0058] (4) Place a photomask on the prepared polydopamine-modified continuous gold film chip, in the ultraviolet cleaning instrument with Hg-Xel light source, set the power 110W, t...

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Abstract

The invention relates to a preparation method of a micro-well structure SPRi chip, a product and application thereof, and belongs to the field of biological detection. The preparation method comprisesthe following steps: firstly, growing a polydopamine film with the thickness of 15-100 nm on a gold film chip, and then placing a mask to form the micro-well structure SPRi chip under the irradiationof ultraviolet light. Efficient SPRi multi-component detection and parallel analysis can be realized, surface electron resonance waves are converged in a detection area (micro-well), the SPRi detection intrinsic sensitivity of the area is improved, meanwhile, background signals are reduced, in addition, a micro-array dot matrix with a clear area is further constructed, and extraction and analysisof detection signals are facilitated.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a preparation method of a SPRi chip with a microwell structure and its product and application. Background technique [0002] Surface plasmon resonance imaging (SPRi) is a new type of optical detection technology, which has the advantages of real-time online, label-free, and parallel detection. It has been widely used in DNA analysis, immunoassay, and biomolecular interactions in recent years. In terms of biochemical analysis such as research, it has important applications in basic research and drug screening, food testing, environmental monitoring and other application fields. At present, there are two main ways to use SPRi for multi-component parallel analysis: one is to directly use the patterned gold island array chip, immobilize different probe molecules on each gold island, and perform multi-component parallel detection; the other is Using ordinary continuou...

Claims

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Application Information

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IPC IPC(8): G01N21/552G01N21/01
CPCG01N21/553G01N21/01
Inventor 胡卫华梅一鸿
Owner SOUTHWEST UNIVERSITY
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