A microbial sensor for detecting biomarkers, a detection method, a culture and detection chip, and a detection system

Abstract

The invention provides a microbial sensor for detecting biomarkers, which is composed of component A and component B. Component A is a conjugate of magnetic beads and nucleic acid aptamers, and the nucleic acid aptamers can be combined with target biomarkers Specific binding; Component B is a genetically engineered bacterial solution transformed with fluorescent protein and expressing resistance, and the nucleic acid probe is modified on the genetically engineered strain. The entire nucleic acid sequence of the nucleic acid probe is complementary to the partial sequence of the nucleic acid aptamer, the target biomarker competes with the nucleic acid probe for binding to the binding site of the nucleic acid aptamer, and the content of the target biomarker is detected by fluorescence intensity. The present invention develops an accurate and sensitive nucleic acid aptamer-coupled microbial fluorescent probe on the basis of establishing the modification and characterization technology of nucleic acid probes on the surface of genetically engineered microorganisms, and the competitive reaction between biomarkers and nucleic acid aptamers and microbial fluorescent probes. New technology for the quantitative detection of needle biomarkers.

Description

technical field [0001] The invention belongs to the field of biomedical detection, and in particular relates to a microbial sensor for detecting biomarkers, a detection method, a culture and detection chip and a detection system. Background technique [0002] Biomarkers are molecules that change significantly during the course of disease, which can be nucleic acids, proteins, metabolites, isoenzymes or hormones, and are divided into diagnostic biomarkers, prognostic biomarkers and predictive biomarkers . The development of accurate and sensitive biomarker quantitative detection technology is not only of great significance for the early diagnosis of diseases, but also can monitor the progress of diseases in real time for timely treatment. [0003] Commonly used detection methods for protein biomarkers include enzyme-linked immunosorbent assay (ELISA), electrochemical analysis, chemiluminescence immunoassay, and immunofluorescence techniques. These detection methods have the...

AI Technical Summary

Problems solved by technology

These detection methods have their own advantages and disadvantages. It is worth mentioning that most methods are based on the immune reaction between antigens and antibodies, while antibodies have disadvantages such as poor stability, difficult modification, and high cost.

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