Unlock instant, AI-driven research and patent intelligence for your innovation.

Purification and adsorption material, and application thereof in simultaneous detection of ten insecticide residues in tomatoes

A technology for adsorption materials and pesticides, which is applied to the purification of adsorption materials and its application in the simultaneous detection of ten kinds of pesticide residues in tomatoes, can solve the problems of large detection errors, and the recovery rate of addition does not meet the requirements, etc., to achieve The effect of improving detection efficiency, improving the accuracy of results, and reducing detection costs

Active Publication Date: 2020-02-04
SHANDONG AGRICULTURAL UNIVERSITY
View PDF3 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Even with the emergence of new materials, the current purification and adsorption materials and pretreatment methods still have the problem that the recovery rate of addition does not meet the requirements, and the detection error is relatively large

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Purification and adsorption material, and application thereof in simultaneous detection of ten insecticide residues in tomatoes
  • Purification and adsorption material, and application thereof in simultaneous detection of ten insecticide residues in tomatoes
  • Purification and adsorption material, and application thereof in simultaneous detection of ten insecticide residues in tomatoes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Embodiment 1 UPLC-MS / MS optimal analysis condition optimization

[0064] Accurately weigh a certain amount of 10 kinds of pesticide technicals, use acetonitrile as a solvent, prepare a single standard solution of 0.5 mg / L for mass spectrometry tuning, and obtain suitable analysis conditions by liquid chromatography and mass spectrometry. Accurately weigh a certain amount of 10 kinds of insecticides to the same volumetric flask, dissolve and constant volume with chromatographically pure acetonitrile solvent, obtain 10 kinds of insecticides mixed standard samples, the concentration is 500mg / L, and then dilute step by step, 100, 50, 10, 5, 1, 0.5 μg / L mixed standard samples were obtained, and after analysis and detection, standard curves of 10 pesticides were obtained.

[0065] Pump 0.5 mg / L standard solution for tuning into the mass spectrometer, and use ESI+ mode to determine the parent ions and product ions of 10 pesticides to be tested respectively; select two ion pair...

Embodiment 2

[0073] 9 kinds of pretreatment method screening of 10 kinds of insecticides in embodiment 2 tomato

[0074] Crush the tomato sample, accurately weigh 10.00g of the tomato sample into a 50mL centrifuge tube, add 10 kinds of insecticide mixed standard samples, so that the addition level in each centrifuge tube is 0.02mg / kg, after standing for 2h, respectively Add 20mL of acetonitrile as the extraction solvent, vortex and mix to form a uniform sample solution, so that the sample is fully dissolved and extracted; add 2g of anhydrous Na 2 SO 4 and 3gNaCl, vortexed for 10s, centrifuged at 4000rpm for 5min, took 1.5mL supernatant, used 9 different adsorbent combinations for QuEChERS purification treatment, vortexed and mixed, centrifuged at 12000rpm for 5min, took the supernatant and filtered it with a 0.22μm organic filter membrane, to be tested. The addition recoveries corresponding to the 9 adsorbent combinations were compared, and each adsorbent combination was repeated twice. ...

Embodiment 3

[0082] The matrix effect of ten kinds of insecticides in the tomato of embodiment 3

[0083] In order to evaluate the matrix effect of 10 insecticides in tomato, a blank matrix solution (as a solvent for dilution) was prepared with the best adsorbent combination material screened out, and mixed standard samples of 10 insecticides were used to obtain 0.5, 1, 5 , 10, 50, and 100 μg / L matrix mixed standard samples, calculate the matrix standard curves of 10 pesticides respectively, and make the solvent standard curves (acetonitrile samples) of 10 pesticides at the same time, using the matrix standard curve and solvent standard curve The slope ratio of is used as the matrix effect (ME) evaluation index. Generally speaking, when ME is between 0.8 and 1.2, it is considered that there is no obvious matrix effect. The closer the ratio is to 1, the weaker the matrix effect is; when the ratio is greater than 1, it is a matrix enhancement effect;

[0084] The acetonitrile solution stand...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a purification and adsorption material, and an application thereof in simultaneous detection of ten insecticide residues in tomatoes, and belongs to the field of pesticide residue analysis. The purification and adsorption material is composed of PSA, anhydrous magnesium sulfate and carbon nanotubes according to a mass ratio of (22-28):(72-78):(0.5-4.0). A tomato sample is subjected to salting-out and vortex treatment by taking acetonitrile as an extraction solvent, and then is subjected to high-speed refrigerated centrifugal layering, and the obtained supernatant is taken, is purified by using a purification adsorbent, is filtered by using a 0.22 [mu] organic filter, and is analyzed and detected by using UPLC-MS / MS. The purification and adsorption material realizeseffective pretreatment of a sample matrix of tomatoes with a high sugar content and a high viscosity, and well separates a to-be-detected sample and the sample matrix; and the application of the purification and adsorption material achieves simultaneous detection of ten pesticide residues in the tomato sample, remarkably improves the detection efficiency and remarkably reduces the detection cost.

Description

technical field [0001] The invention relates to the field of pesticide residue analysis, in particular to a purification adsorption material and its application in simultaneous detection of ten kinds of pesticide residues in tomato. Background technique [0002] Tomatoes are the most widely planted vegetables in the world. They are indispensable in daily life. In addition to being eaten directly, they can also be made into processed products such as canned food, tomato sauce, and tomato juice. During tomato planting, common pests and diseases on tomatoes, such as aphids, whiteflies, root-knot nematodes and Liriomyza sativae, etc., will have a greater impact on the growth of tomatoes, and in severe cases will lead to a large amount of yield reduction. Pesticides are often used for pest control. At present, the following 10 insecticides are commonly used on tomatoes: acetamiprid, imidacloprid, nitenpyram, flupirfuranone, thiamethoxam, phoxim, chlorpyrifos, and spirulina ester...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/22G01N30/14
CPCB01J20/045B01J20/205B01J20/22G01N30/14G01N2030/143G01N2030/146
Inventor 林琎李北兴慕卫刘峰邹楠杨松
Owner SHANDONG AGRICULTURAL UNIVERSITY