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Extraction process of biological crude alkali from leaves of jatropha curcas

An extraction process and technology of jatropha leaves, applied in the field of biotechnology, can solve problems such as less research on jatropha bioalkaloids

Inactive Publication Date: 2020-02-07
凉山德农生物能源股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on Jatropha alkaloids

Method used

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  • Extraction process of biological crude alkali from leaves of jatropha curcas
  • Extraction process of biological crude alkali from leaves of jatropha curcas
  • Extraction process of biological crude alkali from leaves of jatropha curcas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Such as figure 1 As shown, the jatropha leaves were air-dried naturally. Use a pulverizer to crush, sieve, size ≤ 5.0mm, and store at room temperature. Take 1.0 kg of Jatropha jatropha leaves, disperse them in 10 L of absolute ethanol, and process them ultrasonically while stirring. The ultrasonic power is 500 W, and the ultrasonic time is 20 minutes. Remove the residue by filtration, remove ethanol from the obtained yellow solution, add 1.0L of 10% acetic acid aqueous solution, soak for 0.5h, completely dissolve the acidified alkaloid in water, and filter with filter paper to obtain a yellow water phase. This process was repeated twice, and the yellow aqueous phases were combined and concentrated together.

[0028] Add 10% sodium hydroxide aqueous solution to the filtered aqueous phase to adjust the pH value to about 10. Put the combined 3.0L aqueous phase in a suction filter bottle containing filter cloth, vacuum pump suction to remove residue, the pressure of the ...

Embodiment 2

[0030] The jatropha leaves were dried for 18 hours at 60°C in a dryer. Use a pulverizer to crush, sieve, size ≤ 2.0mm, and store at room temperature. Take 1.0 kg of Jatropha Jatropha leaves, disperse them in 10L ethanol / water (95:5), and perform ultrasonic treatment while stirring. The ultrasonic power is 500W, and the ultrasonic time is 20min. Remove the residue by filtration, remove ethanol from the obtained yellow solution by rotary evaporation, add 950 mL of 10% acetic acid aqueous solution to completely dissolve the acidified alkaloid in water, and filter with filter paper to obtain a yellow aqueous solution. This process was repeated twice, and the yellow aqueous phases were combined and concentrated together.

[0031] Add 10% sodium hydroxide aqueous solution to the filtered aqueous phase to adjust the pH value to about 10. Put the combined 3.0L aqueous phase in a suction filter bottle containing filter cloth, vacuum pump to remove residue, the pressure of the vacuum ...

Embodiment 3

[0033] Jatropha leaves were dried for 12 hours at 70°C in a dryer. Use a pulverizer to crush, sieve, size ≤ 3.0mm, and store at room temperature. Take 2.0kg Jatropha leaves, disperse them in 20L ethanol / water (90:10), and ultrasonicate while stirring. The ultrasonic power is 300W, and the ultrasonic time is 30min. Remove the residue by filtration, remove ethanol from the obtained yellow solution by rotary evaporation, add 1.9 L of 8% acetic acid aqueous solution to completely dissolve the acidified alkaloid in water, and filter with filter paper to obtain a yellow aqueous solution. This process was repeated twice, and the yellow aqueous phases were combined and concentrated together.

[0034] Add 20% sodium hydroxide aqueous solution to the filtered aqueous phase to adjust the pH value to about 10. Put the combined 6.0L aqueous phase in a suction filter bottle containing filter cloth, vacuum pump to remove the residue, the pressure of the vacuum pump is 0.5MPa, and then extr...

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Abstract

The invention provides an extraction process of biological crude alkali from leaves of jatropha curcas, and belongs to the technical field of biotechnology. The extraction process comprises the following steps that a, hoarded leaves of the jatropha curcas are naturally air-dried or dried by a dryer, and is smashed by adopting a pulverizer, the size is less than or equal to 5 mm after sieving, andstorage is conducted at room temperature; b, 1.0-5.0 kg of the sieved leaves of the jatropha curcas is dispersed into 10-50 L of an ethanol / water mixed solution for extraction, and ultrasonic treatment is conducted while stirring; c, residues are removed by filtration, after ethanol of an obtained yellow solution is removed by rotary evaporation, 1-5 L of an acetic acid aqueous solution or a hydrochloric acid aqueous solution is added for immersing for 0.5-1.0 h; and filtering is conducted to obtain yellow water phase; d, the above steps are repeatedly conducted for 2-4 times; e, an alkalineaqueous solution such as sodium hydroxide is added into water phase obtained in the step d, and the pH value is adjusted to about 10.0; and f, the combined mixed solution in the step e is filtered orsubjected to suction filtration by a vacuum pump, and then is extracted for 3 times with a water-insoluble organic solvent with equal volume and low boiling point, and the combined organic phase is dried, filtered and rotary-evaporated to obtain the biological crude alkali.

Description

technical field [0001] The invention belongs to the technical field of biotechnology, and in particular relates to a process for extracting biological crude alkali from jatropha cotyledons. Background technique [0002] The common name of Jatropha Jatropha, Jatropha, Black Soap Tree, Wood Peanut, Oil Luzi, Laopangguo, Mahonghan, etc., belongs to Euphorbiaceae Jatropha, which is a deciduous shrub or small tree. The leaves of Jatropha curcas can be used as medicine. It is bitter in nature and flavor, slightly cold, and poisonous. Scholars at home and abroad have carried out a lot of research on the chemical constituents of Jatropha. So far, using a variety of separation materials and separation techniques, the reported bioactive components include terpenoids, lignin, coumarins, flavonoids, alkaloids, proteins, lignans, and phytosterols. compound. Diterpenes are the main components of this genus of plants, among which leprosin has anti-leukemia and anti-nasopharyngeal cancer...

Claims

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Application Information

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IPC IPC(8): B01D11/02B01D11/04B01J19/10
CPCB01D11/0265B01D11/0288B01D11/0492
Inventor 曾伟赵丽娟易龙飞宋永娇高元吉史英博张欣汝
Owner 凉山德农生物能源股份有限公司
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