Method for vermiremediation synergy of polycyclic aromatic hydrocarbon-contaminated soil
A polycyclic aromatic hydrocarbon and polluted soil technology, which is applied in the field of polluted environment remediation, can solve the problem of low efficiency of worm remediation technology, and achieve the effects of improving sustainable utilization ability, improving biological effectiveness and simple process
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[0028] Preparation of PAH-contaminated soil: Add fluoranthene acetone solution to 100g of soil, let it stand overnight in a dark place and mix it with 400g of non-polluted soil to make the final concentration of fluoranthene reach 100mg / kg (25°C) 50mg / kg and 100mg / kg. The settings are handled as follows, as shown in Table 1:
[0029] Table 1 Experimental setup table
[0030]
[0031]
[0032] Weigh 500g and put it in a large beaker, adjust the soil water content to 50% of the field water holding capacity, and balance for 7 days; put about 20g of adult earthworms (about 40) into the beaker, and take destructive sampling on 15 days. After picking out the earthworms, monitor the concentration and form distribution of the pollutant fluoranthene in the soil, the accumulation of fluoranthene in the earthworm body and the antioxidant defense system in the earthworm body, and evaluate the restoration effect and the survival status of the earthworm. See the results separately ...
Embodiment 1
[0034] A method for worm remediation and efficiency enhancement of polycyclic aromatic hydrocarbon-contaminated soil, comprising the following steps:
[0035] Step 1. Add Tween-80 solution to the polycyclic aromatic hydrocarbon-contaminated surface soil, the dosage of the Tween-80 solution is to add 10 mg per 1 kg polycyclic aromatic hydrocarbon-contaminated surface soil, and plow evenly;
[0036]Step 2. Adjust the field water holding capacity of the PAH-contaminated surface soil in step 1 to 50%, and spray the Tween-80 aqueous solution of 10mg / L×20L per square meter to the soil surface, and activate it for 10 days;
[0037] Step 3. Inoculation density is 0.9kg / m in the PAH-contaminated surface soil after activation in step 2 2 The adult chrysalis earthworms with reproductive links, and the inoculated adult chrysalis earthworms with reproductive links are managed in the field according to conventional methods;
[0038] Step 4. Turn the adult chizi love earthworm with the repr...
Embodiment 2
[0041] A method for worm remediation and efficiency enhancement of polycyclic aromatic hydrocarbon-contaminated soil, comprising the following steps:
[0042] Step 1. Add rhamnosyl ester solution to the surface soil polluted by polycyclic aromatic hydrocarbons, the dosage of the rhamnosyl ester solution is to add 20 mg per 1 kg of polycyclic aromatic hydrocarbons polluted surface soil, and plow evenly;
[0043] Step 2. Adjust the field water holding capacity of the PAH-contaminated surface soil in step 1 to 70%, spray the rhamnosyl ester aqueous solution of 10mg / L×20L per square meter on the soil surface, and activate it for 20 days;
[0044] Step 3. Inoculation density is 1.5kg / m in the PAH-contaminated topsoil after activation in step 2 2 The adult chrysalis earthworms with reproductive links, and the inoculated adult chrysalis earthworms with reproductive links are managed in the field according to conventional methods;
[0045] Step 4. Turn the adult chizi love earthworm ...
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