Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for rapidly detecting content of hydrogen peroxide

A hydrogen peroxide and content technology, applied in measuring devices, material analysis by optical means, instruments, etc., can solve the problems of expensive instruments, complicated pretreatment, long operation time, etc., and achieve the effect of non-destructive quantitative analysis

Pending Publication Date: 2020-02-11
启东科赛尔纳米科技有限公司
View PDF9 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above methods can accurately quantitatively analyze hydrogen peroxide, they all have some disadvantages such as complicated pretreatment, expensive instruments, long operation time, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for rapidly detecting content of hydrogen peroxide
  • Method for rapidly detecting content of hydrogen peroxide
  • Method for rapidly detecting content of hydrogen peroxide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0028] 1. Instruments, reagents and instrument working conditions

[0029] 1) Instrument

[0030] Confocal Raman spectrometer (XploRA Plus, 638nm)

[0031] 2) Reagent

[0032] Chlorauric Acid, Sodium Citrate, Hydrogen Peroxide, Glucose, 4-Mercaptophenylboronic Acid, Sodium Silicate, 3-Aminopropyl-Trimethoxysilane (APTMS)

[0033] 3) Raman Spectroscopy Conditions

[0034] The parameters of the Raman spectrometer are set as: XploRA Plus of HORIBA Company, light source: 638nm; power: 1.6mW; scanning range: 350-2000cm -1 ; Grating: 1200T; 10 spectra were collected for each sample, and the average value was taken.

[0035] 2. Preparation of standard solution

[0036] First, accurately prepare the mother solution of hydrogen peroxide aqueous solution with a concentration of 100M, and dilute them respectively to obtain standard aqueous solutions of hydrogen peroxide with concentrations of 9M, 7M, 5M, 3M and 1M.

[0037] 3. Preparation of SERS chip

[0038] 1) Preparation of co...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Thicknessaaaaaaaaaa
Login to View More

Abstract

The invention discloses an SERS chip for rapidly detecting the content of hydrogen peroxide. The SERS chip comprises a substrate and reinforced nano particles added on the substrate dropwise, phenylboronic acid molecules are modified on the substrate, and the reinforced nano particles comprise gold nano particles and inert shell layers wrapping the surfaces of the gold nano particles. The invention also provides a method for rapidly detecting the content of hydrogen peroxide by using the SERS chip. The method comprises the following steps: dripping a solution containing a hydrogen peroxide metabolite on the SERS chip, carrying out a Raman spectrum test to obtain a Raman spectrum after reaction of hydrogen peroxide and phenylboronic acid molecules, and comparing the Raman spectrum with a standard curve to calculate the content of hydrogen peroxide. The detection method provided by the invention has the advantages of simple pretreatment, rapidness, high efficiency and strong specificity.

Description

technical field [0001] The invention relates to the technical field of rapid detection of Raman spectroscopy, in particular to a method for rapidly detecting the content of hydrogen peroxide, a cell metabolite, using surface-enhanced Raman spectroscopy. Background technique [0002] Reactive oxygen species (ROS), as a class of highly reactive chemicals, play a very important role in physiological and pathological processes. The so-called reactive oxygen species (ROS), in a nutshell, refers to the general term for substances that are composed of oxygen in the body or in the natural environment, contain oxygen and are active in nature. The lack of active oxygen in the body may induce certain diseases (such as chronic granulomatous disease, certain autoimmune diseases, etc.); and the excessive production of active oxygen in the body can also induce diseases (such as cancer, arthritis, arteriosclerosis, etc.). ROS in living organisms include neutral molecules (eg: H 2 o 2 , ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 李剑锋温宝英
Owner 启东科赛尔纳米科技有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com