Method for biosynthesizing nano-silver bacteriostatic agent by utilizing Sibiraea angustata extract

A technology of biosynthesis and silver antibacterial agents, applied in the field of biosynthesis of nano silver antibacterial agents, can solve problems such as difficult separation and utilization, difficulty in extraction methods, plant resources, medical resources and economic loss and waste, and achieve a broad antibacterial spectrum , not easy to drug resistance, enhance the effect of antibacterial effect

Active Publication Date: 2020-02-21
NORTHWEST UNIVERSITY FOR NATIONALITIES
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Tibetan medicine willow tea is rich in antioxidant substances and antibacterial substances. However, due to the difficulty of its extraction method, it is difficult to separate and utilize it, so that it cannot exert its powerful antioxidative and antibacterial pharmacological effects, resulting in huge plant resources, medical resources and Economic loss and waste, the development and utilization of willow tea nano-silver will have great significance and economic value for the development and utilization of willow tea, a unique plant resource of the nation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for biosynthesizing nano-silver bacteriostatic agent by utilizing Sibiraea angustata extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Material preparation: collecting Tibetan medicine willow tea leaves, cleaning and drying to constant weight, to obtain dry willow tea leaves;

[0024] (2) Tibetan medicine willow tea leaves are placed in the shade under natural conditions, dried in a blast oven at 45°C, crushed and passed through a 40-mesh sieve to obtain Tibetan medicine willow tea leaf powder, and 200g of Tibetan medicine willow tea leaf powder is accurately weighed and respectively mixed with Mix sterile water and 70% ethanol at room temperature, heat and reflux at 100°C for 30 minutes, repeat 3 times, combine the extracts for 3 times, evaporate in vacuum at 35°C to obtain the extract of Tibetan medicine willow tea extract, dilute it to 100mg with 70% ethanol / mL;

[0025] (3) Preparation of Tibetan medicine willow tea nano-silver antibacterial agent: take Tibetan medicine willow tea extract and AgNO 3 Mix the solution, the mixing ratio is 30:1 according to the mass ratio of the solution, then a...

Embodiment 2

[0033] (1) Material preparation: collecting Tibetan medicine willow tea leaves, cleaning and drying to constant weight, to obtain dry willow tea leaves;

[0034] (2) Preparation of Tibetan medicine willow tea extract: 45 DEG C blast oven drying, crushed and passed through a 40-mesh sieve to obtain Tibetan medicine willow tea leaf powder, accurately weighed 200g of Tibetan medicine willow tea leaf powder and mixed with sterile water, Mix 70% ethanol at room temperature, heat and reflux at 100°C for 30 minutes, repeat 3 times, combine the extracts for 3 times, evaporate in vacuum at 35°C to obtain the extract of Tibetan medicine willow tea extract, dilute it to 100mg / mL with 70% ethanol;

[0035] (3) Refining and purification: mix the antibacterial agent obtained in step (2) with bentonite and diatomaceous earth, add it into a three-necked flask, heat to 100°C, reflux for 80min, remove the filtrate and filter residue after reflux, and remove the filter residue and supernatant Re...

Embodiment 3

[0041] The antibacterial agent is a single nano AgNO 3 Solution bacteriostatic agent.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for biosynthesizing a nano-silver bacteriostatic agent by utilizing a Sibiraea angustata extract. The method comprises the following steps: (1) preparation of materials; (2) preparation of the Sibiraea angustata extract; (3) preparation of the nano-silver bacteriostatic agent of the Sibiraea angustata extract; (4) refining and purifying; (5) filtering with macroporous resin; etc. According to the invention, rich antioxidant substances extracted from Sibiraea angustata are used as a reducing agent, and a one-step reduction method is adopted for preparation of thenano-silver bacteriostatic agent; meanwhile, a bacteriostatic effect is synergistically improved through the antibacterial effect of the Sibiraea angustata, and the bacteriostatic effect is excellent.

Description

technical field [0001] The invention relates to a method for biosynthesizing a nano-silver antibacterial agent by utilizing the extract of Tibetan medicine willow tea, belonging to the field of natural product extraction. Background technique [0002] Silver is a metal inorganic fungicide with a long history of application. Silver nanoparticles (AgNPs) are simple substances with a nanometer-scale particle size of metallic silver. Most of the particle size of nano-silver is about 25nm. Dozens of pathogenic microorganisms such as Chlamydia trachomatis have a strong inhibitory and killing effect, and will not produce drug resistance. The antibacterial mechanism of nano-silver is: silver element or silver ion has a positive charge, which can combine with negatively charged microbial cell membranes, change the permeability of the membrane, and combine with bacterial proteins and nucleic acids, destroying the ability of microorganisms to divide and proliferate, and eventually lead...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/34A01N59/16A01P1/00A01P3/00A61K36/73A61K33/38A61P31/04A61P31/10
CPCA01N59/16A01N65/00A01N65/34A61K33/38A61K36/73A61P31/04A61P31/10A61K2300/00Y02A50/30
Inventor 郭鹏辉边缘臧荣鑫曹忻徐红伟杨具田高丹丹韦体赫雪吴登宇
Owner NORTHWEST UNIVERSITY FOR NATIONALITIES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap