Fermentation culture medium for antigen expression of foot-and-mouth disease virus-like particles through Escherichia coli and culture method using fermentation culture medium

Abstract

The invention discloses a fermentation culture medium for antigen expression of foot-and-mouth disease virus-like particles through Escherichia coli and a culture method using the fermentation culturemedium. The fermentation culture medium comprises a basic medium, a material supplementing medium and an induced expression medium, wherein the basic medium can be used for shortening the lag phase of Escherichia coli strains for production so that the strains enter a logarithmic growth phase as soon as possible, the material supplementing medium can be used for increasing the multiplication capacity of Escherichia coli in the logarithmic growth phase and ensuring that a higher microbial content of Escherichia coli is achieved in a high-density fermentation process, and the induced expressionmedium is used for maintaining the plateau phase of Escherichia coli so as to increase the expression amount of antigens of the virus-like particles; and meanwhile expression of the antigens in a soluble form is promoted by components during culture. The fermentation culture method includes culture of the production strains, expansion culture of the strains, induction expression and other steps.Through the fermentation culture medium and culture method, the expression amount of the antigens of the virus-like particles can be increased greatly, and at the same time expression of the antigensin a soluble form can be achieved to a maximum extent.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a fermentation medium and a culture method for expressing foot-and-mouth disease virus-like particle antigens in Escherichia coli. Background technique [0002] Foot-and-mouth disease is an acute, febrile, highly contagious animal disease caused by Foot-and-Mouth Disease Virus (FMDV), mainly in cloven-hoofed animals. The disease is currently the most severe infectious disease threatening the development of animal husbandry in the world, so the World Organization for Animal Health (OIE) lists it as an infectious disease that must be reported. The attenuated vaccine once played a huge role in controlling the epidemic of foot-and-mouth disease in Europe, but it was suspended due to the strong virulence of the vaccine strain which caused the outbreak of foot-and-mouth disease. Although the currently used inactivated vaccines are safer than the attenuated vaccines, if...

AI Technical Summary

Problems solved by technology

When antigen expression is performed in the E. coli system, the antigen expression level is too low, which greatly increases production costs while reducing production capacity; if the antigen expression level is too high, the possibility of antigen expression in the form of inclusion bodies is greatly increased, which increases the difficulty of subsequent purification and cost, affecting in vitro assembly efficiency

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