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Biomarker group, application thereof, protein chip, protein chip kit and ELISA kit

A biomarker and protein chip technology, applied in the field of biomedicine, can solve the problems of incomplete compliance with related diseases, clinical involvement limitations, etc., and achieve the effect of less specimen consumption and overcoming cumbersome operations.

Active Publication Date: 2020-02-28
RAYBIOTECH INC GUANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2010, the European Federation of Neurology (EFNS) clearly defined NMOSD, specifically referring to a group of related diseases with similar potential pathogenesis to NMO, but limited clinical involvement and not fully in line with the diagnosis of NMO
[0037]Currently, there is no reliable biomarker for the diagnosis of multiple sclerosis (MS) and neuromyelitis spectrum disease (NMOSD) under the negative condition of aquaporin 4 antibody objects, as well as protein chips and kits

Method used

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  • Biomarker group, application thereof, protein chip, protein chip kit and ELISA kit
  • Biomarker group, application thereof, protein chip, protein chip kit and ELISA kit
  • Biomarker group, application thereof, protein chip, protein chip kit and ELISA kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] The embodiment of the present invention is a serum sample preparation process, and the specific steps are as follows:

[0090] 1. A total of 12 patients with multiple sclerosis were collected, and 9 patients with aquaporin 4 antibody (AQP4 antibody) negative neuromyelitis spectrum disease (AQP4 Ab-seronegative NMOSD) were collected. The following are called MS group and NMO .NEG group. The differential diagnosis of multiple sclerosis (MS) patients and neuromyelitis spectrum disease (NMO) patients is shown in Table 1. All serum samples are processed according to standard procedures: collect whole blood in a vacuum serum tube and let it stand at room temperature for 30 minutes , And then centrifuge at 2000 rpm in a 4°C centrifuge for 15 minutes. Collect the supernatant and freeze at -80°C until use. All clinical serum samples used in this example were approved by the ethics review committee of human subjects in the Second Affiliated Hospital of Guangzhou Medical University...

Embodiment 2

[0094] The embodiment of the present invention is to detect the serum protein expression level of the AQP4 antibody-negative multiple sclerosis patient and the AQP4 antibody-negative neuromyelitis spectrum disease patient of Example 1. The specific steps are as follows:

[0095] 1. Protein chip technology to determine the expression levels of 200 proteins in the serum of patients with AQP4 antibody-negative multiple sclerosis and AQP4 antibody-negative neuromyelitis spectrum diseases in Example 1: Use sandwich-based antibody microarray to measure serum Protein levels in. RayBiotech Human Cytokine Antibody ArrayQ4000 (QAH-CAA-4000, RayBiotech, Inc., Norcross, GA) was used for protein screening, which included a combination of 5 non-overlapping arrays to quantitatively measure 200 human cytokines.

[0096] The preparation steps of the protein chip of the present application are as follows:

[0097] 1. Complete drying of the slide chip: Take the slide chip out of the box and equilibrat...

Embodiment 3

[0121] The examples of this application provide protein data conversion and graph generation of Example 2. The specific steps are as follows:

[0122] After calculating the concentration of the 8 biomarkers in this application according to the specific software of the protein chip, perform data conversion before data analysis. The conversion formula is: log 2 (X+1). X is the concentration of different proteins corresponding to each sample. The transformed data is used for subsequent data analysis, including volcano map analysis and heat map cluster analysis. All data is directly generated in RStudio, and then the pictures are integrated using Illustrator CC5 (Adobe, San Diego, CA).

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PUM

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Abstract

The invention belongs to the technical field of biological medicines, and particularly relates to a biomarker group, application thereof, a protein chip, a protein chip kit and an ELISA kit. The provided biomarker group is applied to preparation of a product for diagnosing demyelinating diseases of the central nervous system, and comprises a stromal cell derivative factor-1, granulocyte chemotactic protein 2, mononuclear cell chemotactic protein-1, interleukin-2, gamma-interferon, insulin-like growth factor binding protein-3, E-cadherin and macrophage inflammatory protein-1 delta; and the demyelinating diseases include an aquaporin 4 antibody negative neuromyelitis spectrum disease and an aquaporin 4 antibody negative multiple sclerosis. The blank there is no reliable product and method for diagnosing and identifying multiple sclerosis and neuromyelitis spectrum diseases under negative aquaporin 4 antibody clinically at present is filled.

Description

Technical field [0001] The invention belongs to the technical field of biomedicine, and particularly relates to a biomarker group and its application, as well as a protein chip, a protein chip kit and an ELISA kit. Background technique [0002] Central nervous system demyelinating disease is an autoimmune system disease mainly characterized by multifocal and inflammatory demyelination of the central nervous system. Its clinical features mainly include repeated attacks, multiple remissions and relapses. Common clinical demyelinating diseases of the central nervous system include multiple sclerosis (MS), neuromyelitis optica (NMO), acute disseminated encephalomyelitis, etc. The incidence of demyelinating diseases of the central nervous system has been increasing year by year in recent years The trend has a serious impact on the quality of life of patients and poses a serious threat to the physical and mental health of patients. [0003] MS and NMO are two demyelinating diseases of t...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/558
CPCG01N33/6854G01N33/558G01N2800/285
Inventor 符聪聪吕瑞黄若磐
Owner RAYBIOTECH INC GUANGZHOU
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