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Track regeneration type DNA walker and application thereof

A walker and track technology, applied in the direction of recombinant DNA technology, DNA / RNA fragments, biochemical equipment and methods, etc., can solve the problems of unfavorable walker operation, limit the number of walker steps, etc., to enhance continuous operation, increase Effects of walking steps, rapid drug delivery

Active Publication Date: 2020-03-24
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the existing design of DNA walkers, as the walking chain walks, the track for the walking chain to walk will be gradually exhausted due to the replacement of the chain or the consumption of hydrolysis, which severely limits the walking ability of the walker. The number of steps taken, which is not conducive to the continuous operation of the walker along the track

Method used

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  • Track regeneration type DNA walker and application thereof
  • Track regeneration type DNA walker and application thereof
  • Track regeneration type DNA walker and application thereof

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Embodiment 1

[0051] 1.1 Chemicals and reagents

[0052] All oligonucleotides were purchased from Shanghai Sangon Biotechnology Co., Ltd., and their sequences are listed in Table 1. Exonuclease III (Exo III) used at a concentration of 100000 U mL -1 ) was purchased from New England Biolabs Ltd. (Beijing, China). All chemicals used were of analytical purity and were used without further purification. Throughout the experiment, 18.25 MΩcm Milli-Q ultra-pure water was used.

[0053] Table 1. Oligonucleotide sequences used in this work

[0054]

[0055]

[0056] [a] GenBank: AF086833.2. [b] GenBank: KU182911.1. [c] GenBank: FJ968794.1. [d] GenBank: MF540570.1. [e] GenBank: MH121167.1.

[0057] 1.2 Instruments

[0058] UV-Vis absorption spectra were recorded with a U-2910 UV-Vis spectrophotometer (Japan). Fluorescence emission spectra were collected on a Hitachi F-7000 fluorescence spectrophotometer (Japan). Its excitation wavelength is 488nm, and its emission spectrum ranges from 5...

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Abstract

The invention belongs to the technical field of construction of DNA walkers, and particularly relates to a track regeneration type DNA walker and an application thereof. The concept of the track regeneration type DNA walker is provided and verified. The walker adopts a separable track consisting of a roadbed and a track. The roadbed chain of the walker is functionalized on gold nanoparticles, anda large number of track chains are dissociated in a solution and are hybridized with the roadbed chain to construct the track. As the walker walks along the track, the track chain is digested and releases the roadbed chain at the same time, and the released roadbed chain can be continuously combined with the track chain in the solution to form a new track. The continuous supply of the track enables the walker to keep walking. The walker has great advantages in the aspect of signal enrichment, so that the walker can be used for sensitive detection of Ebola virus gene segments in serum.

Description

technical field [0001] The disclosure belongs to the technical field of DNA walker construction, and in particular relates to a DNA walker with reproducible orbit, the walker and its application in biosensing. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present disclosure, and is not necessarily to be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] DNA molecular machines are a class of molecular machines assembled from DNA that perform machine-like behaviors at the micro or nano scale. Benefiting from the unique properties of DNA molecules, such as strict complementary base pairing, excellent recognition ability, and high programmability, DNA molecular machines can be precisely controlled and finely manipulated in response to various stimuli. To date, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/682
CPCC12Q1/682C12Q2525/301C12Q2563/137C12Q2563/107C12Q2565/519
Inventor 姜玮徐晓文逄寒
Owner SHANDONG UNIV
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