Beauveria bassiana bh001 for preventing and treating curculio arakawai pests and application thereof

A technology of Beauveria bassiana and Oak oak, applied in the field of microorganisms, to achieve the effect of good biocontrol potential

Active Publication Date: 2020-03-27
BEIHUA UNIV
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  • Beauveria bassiana bh001 for preventing and treating curculio arakawai pests and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Example 1, the acquisition and identification of Beauveria bassiana (Beauveria bassiana) bh001

[0035] 1. Collection of entomopathogenic fungi

[0036] The natural infection of pathogenic fungi was collected from the overwintering soil of Quercus eucalyptus in the forest area of ​​Changbai Mountain, Jilin Province.

[0037] 2. Isolation and purification of entomopathogenic fungi

[0038] Carry out surface disinfection with 95% ethanol to the collected oak weevils infected with pathogenic fungi, cut into 0.2mm size tissue pieces with a knife, put them on PDA plate culture medium, and cultivate them at a constant temperature of 25°C. Purification is carried out to obtain pure strains.

[0039] 3. Determination of strain virulence

[0040] (1) Collect wild oak weevil larvae, and pick healthy and lively individuals for experiments.

[0041] (2) Add 20ml of TW-80 solution with a concentration of 0.05% (volume percentage) into 50ml conical flasks, then scrape the culture...

Embodiment 2

[0061] Embodiment 2, control test of Beauveria bassiana (Beauveria bassiana) bh001 to oak oak

[0062] 1. Indoor oak weevil control experiment

[0063] (1) Preparation of biocontrol bacteria

[0064] Beauveria bassiana (Beauveria bassiana) bh001 is used as the production strain, and the mixture of wheat bran, rice bran and corn grits (3:1:1) (mass ratio) is used as the culture substrate, after inoculation, cultivation, drying, crushing and other processing Made into powder, the spore content is 10 billion (±10) billion / g, and the fineness is all through 35 mesh sieves.

[0065] (2) Oak oak weevil larva preparation

[0066] The larvae of Quercus mongolica were collected from the fruits of Quercus mongolica collected in the field, and a 1m×1m×0.5m wooden box was prepared, filled with soil. 30 repetitions.

[0067] (3) Powder spraying test

[0068] Spraying steps (1) Spray 2-3g of Beauveria bassiana powder per square meter, and then press 1m on the soil surface 2 Place 100 ...

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Abstract

The invention discloses a strain of beauveria bassiana bh001 for preventing and treating curculio arakawai pests and application thereof. The invention provides the beauveria bassiana bh001 which is preserved in CGMCC (China General Microbiological Culture Collection Center), and the preservation number is CGMCC No.17975. The biocontrol strain of the beauveria bassiana bh001 provided by the invention can be used for effectively preventing and treating curculio arakawai, has a good biocontrol potential, provides new resources for biological control on forest pests of the curculio arakawai, andalso provides help for protecting Mongolian oak seed resources.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of Beauveria bassiana bh001 for controlling oak weevil pests and its application. Background technique [0002] Quercus mongolica forest is an important secondary forest resource in my country. Starch can be extracted from Quercus mongolica seeds. If it can be fully utilized as feed or replace corn to produce industrial ethanol, it will be of great significance to the country's food security and energy security. In recent years, more than 60% of the species of Quercus mongolica have been damaged by oak elephants, causing huge economic losses. At present, there is no effective and environment-friendly control method for oak elephant damage. Therefore, the prevention and control technology of oak weevil based on pathogenic fungi is a major technical problem that needs to be solved urgently. At present, there are more than 170 kinds of entomopathogenic microbial products reg...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P7/04A01G13/00C12R1/645
CPCA01G13/00C12N1/14C12N1/145C12R2001/645
Inventor 孟庆繁由士江陈玉宝
Owner BEIHUA UNIV
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