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Preparation method of coriolus versicolor extract

An extract, Yunzhi technology, applied in the field of preparation of Yunzhi extract, can solve the problems of low extraction rate and many impurities, and achieve the effect of high extraction rate and less impurities

Inactive Publication Date: 2020-04-03
ZHUHAI COLLEGE OF JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the methods for extracting polysaccharides from Yunzhi polysaccharides use water extraction and alcohol precipitation, acid-base extraction, enzymatic extraction, and ultrasonic extraction, etc. These methods generally have the disadvantages of low extraction rate and many impurities.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A preparation method of Yunzhi extract, comprising the steps of:

[0019] S1. Freezing and brittle versicolor versicolor at -15°C for 30 minutes, crushing, drying, passing through a 60-mesh sieve, and degreasing with 95% ethanol to obtain versicolor fruiting body powder;

[0020] S2. Add 8 times of water to the Versicolor versicolor fruit body powder, add cellulase in a proportion of 1.5% by mass, adjust the pH to 6.0, perform enzymatic hydrolysis at 45°C for 30 minutes, and obtain an enzymatic hydrolysis solution;

[0021] S3. Freeze and thaw the enzymatic solution three times under the condition of -80°C / 4°C, each time for 30 minutes, spray dry, and place in an appropriate amount of 2% Na 2 CO 3 In the solution, soak at -0.06Mpa for 10min, return to normal pressure instantly, 45°C, 450w ultrasonic extraction for 30min, suction filtration, repeat ultrasonic extraction once, and dry to obtain the extract. Among them, Na 2 CO 3 The mass ratio of the solution to the sp...

Embodiment 2

[0023] A preparation method of Yunzhi extract, comprising the steps of:

[0024] S1. Freezing and brittle versicolor versicolor at -10°C for 30 minutes, crushing, drying, passing through a 60-mesh sieve, and degreasing with 95% ethanol to obtain versicolor fruiting body powder;

[0025] S2. Add 15 times of water to Versicolor versicolor fruiting body powder, add cellulase in a proportion of 1.5% by mass, adjust the pH to 6.0, and perform enzymatic hydrolysis at 55°C for 20 minutes to obtain an enzymatic hydrolysis solution;

[0026] S3. Freeze and thaw the enzymatic solution three times under the condition of -80°C / 4°C, each time for 30 minutes, spray dry, and place in an appropriate amount of 2% Na 2 CO 3 In the solution, soak at -0.08Mpa for 10min, return to normal pressure instantly, 45°C, 450w ultrasonic extraction for 50min, suction filtration, repeat ultrasonic extraction once, and dry to obtain the extract, among which, Na 2 CO 3 The mass ratio of the solution to the...

Embodiment 3

[0028] A preparation method of Yunzhi extract, comprising the steps of:

[0029] S1. Freezing and brittle versicolor versicolor at 0°C for 30 minutes, crushing, drying, passing through a 60-mesh sieve, and degreasing with 95% ethanol to obtain versicolor fruiting body powder;

[0030] S2. Add 11 times of water to Versicolor versicolor fruit body powder, add cellulase in a proportion of 1.5% by mass, adjust the pH to 6.0, and enzymolyze at 50°C for 25 minutes to obtain an enzymolyzed solution;

[0031] S3. Freeze and thaw the enzymatic solution three times at -80°C / 4°C, each time for 30 minutes, spray dry, place in an appropriate amount of 2% Na2C03 solution, soak at -0.07Mpa for 10 minutes, and instantly Return to normal pressure, 45°C, 450w ultrasonic extraction for 40min, suction filtration, repeat ultrasonic extraction once, and dry to obtain the extract, in which Na 2 CO 3 The mass ratio of the solution to the spray-dried powder was 3.5:1.

[0032] Measure the absorbanc...

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PUM

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Abstract

The invention discloses a preparation method of a coriolus versicolor extract, which comprises the following steps: S1, freezing and embrittling coriolus versicolor sporocarp at -15 to 5 DEG C for 30min, pulverizing and drying the coriolus versicolor sporocarp, carrying out screening with a 60-mesh sieve, and carrying out degreasing with 95% ethanol to obtain coriolus versicolor sporocarp powder;S2, adding water which is 8-15 times of the coriolus versicolor sporocarp powder into the coriolus versicolor sporocarp powder, adding cellulase according to a mass percentage of 1-2%, adjusting the pH value to 6.0, and carrying out enzymolysis at 45-55 DEG C for 20-30min to obtain an enzymatic hydrolysate; and S3, repeatedly freezing and thawing the enzymatic hydrolysate for three times, carryingout spray drying, carrying out soaking in a proper amount of 2% Na2CO3 solution under a negative pressure condition for 10min, instantaneously recovering to normal pressure, carrying out ultrasonic extraction at 35-50 DEG C for 30-50min, carrying out suction filtration, repeating ultrasonic extraction once, and carrying out drying to obtain the extract. The method is high in extraction rate, andthe obtained extract contains few impurities.

Description

technical field [0001] The invention belongs to the technical field of natural product chemistry, and in particular relates to a preparation method of versicolor versicolor extract. Background technique [0002] Coriolus versicolor, also known as Coriolus versicolor, Coriolus versicolor, etc., belongs to the phylum Fungi, Basidiomycotina, Polyporaceae, and the genus Coriolus. Yunzhi is sweet and light in taste, slightly cold in nature, has the effects of invigorating the spleen and promoting dampness, relieving cough and asthma, clearing heat and detoxification, and anti-tumor. Yunzhi polysaccharide is the main active ingredient of Yunzhi. It can activate immune cells, improve the immune function of the body, anti-tumor, lower blood fat and antibacterial. It is a safe and effective immune enhancer for humans or animals, and can be used as an additive for health food. [0003] At present, most of the methods for extracting polysaccharides from Yunzhi polysaccharides use wate...

Claims

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Application Information

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IPC IPC(8): C08B37/02
CPCC08B37/0003C08B37/0024
Inventor 孟凡欣吴丽艳林玉苗胡力夫冯薇姚易赵明智王亮
Owner ZHUHAI COLLEGE OF JILIN UNIV
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