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Probiotic clostridium butyricum and application thereof

The technology of a clostridium butyricum and an application method is applied to the probiotic clostridium butyricum and its application field, which can solve the problems of difficulty in reducing the mortality of livestock and poultry, difficult to prevent, high mortality, etc., and achieves improving the economic benefits of breeding and improving health. status, the effect of reducing antibiotic use

Inactive Publication Date: 2020-04-03
山东蔚蓝生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of livestock and poultry breeding, diarrhea diseases have a high mortality rate, and acute diarrhea in livestock and poultry is difficult to prevent with conventional methods, and after acute diarrhea occurs, conventional drug treatment often lags behind, making it difficult to reduce the mortality of livestock and poultry

Method used

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  • Probiotic clostridium butyricum and application thereof
  • Probiotic clostridium butyricum and application thereof
  • Probiotic clostridium butyricum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Clostridium butyricum WL-53 isolation and identification:

[0024] 1. Isolation and purification of Clostridium butyricum

[0025] Collect 10 parts of fresh chicken manure from a chicken farm, weigh 25g samples respectively, add 225mL sterile water to prepare a suspension, spread on an anaerobic bacteria agar plate, and incubate anaerobically at 37°C for 72 hours. Select a single colony to observe the shape of the bacteria one by one, screen out the bacteria that are fusiform under the microscope, and then streak and purify three times to obtain a pure strain.

[0026] 2. Identification of Clostridium butyricum

[0027] 1) Physiological and biochemical characteristics:

[0028] Cultivated on anaerobic agar medium for 72 hours, it appears as off-white, opaque, smooth-surfaced colonies with a diameter of about 1-3 mm; observed by Gram staining, it is positive, opposite rod-shaped, and the spores are fusiform, located in the middle or End; V-P test (-), gelati...

Embodiment 2

[0032] Embodiment 2: Clostridium butyricum WL-53 bacterial liquid preparation

[0033] 1. Seed liquid preparation: Pick a single colony of Clostridium butyricum WL-53 and inoculate it in 50 mL of fresh broth medium. The components are glucose 10 g / L, peptone 10 g / L, sodium chloride 5 g / L, sulfur Sodium glycolate 1g / L, anaerobic culture at 37°C for 48h, which is the seed solution;

[0034] 2. Expansion culture: transfer 10% of the inoculum to fresh broth culture medium, anaerobically culture at 37°C, and when the spore formation reaches more than 90%, stop the culture to make a liquid bacterial agent.

Embodiment 3

[0035] Embodiment 3: Clostridium butyricum WL-53 bacteria powder preparation

[0036] 1. Bacterial liquid collection: the anaerobically cultured Clostridium butyricum WL-53 bacterial liquid in Example 2 was divided into sample bottles, and refrigerated at 4°C to keep the bacterial liquid fresh;

[0037] 2. Cell centrifugation: Set the centrifugation condition to 4000rpm for 10 minutes. After the bacterial liquid is centrifuged, discard the supernatant and collect Clostridium butyricum WL-53 cells for later use;

[0038] 3. Drying: In this example, corn starch was selected as the carrier, and the corn starch and the collected Clostridium butyricum WL-53 cells were mixed according to the ratio of 1:1, dried in a drying oven at 60°C for 24 hours, and then pulverized. Clostridium butyricum WL-53 bacterial powder was obtained.

[0039] 4. Determination of bacterial count of bacterial powder: Weigh 1g of Clostridium butyricum WL-53 bacterial powder, add 9mL of sterile water, shake ...

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Abstract

The invention belongs to the technical field of microorganism application, and relates to probiotic clostridium butyricum and application thereof. The clostridium butyricum is separated from fresh chicken manure of an autotrophic chicken farm, and has a remarkable inhibiting effect on pathogenic escherichia coli and clostridium perfringens of cultured animals. After the clostridium butyricum is subjected to anaerobic fermentation culture, a liquid microbial agent product can be prepared for the cultured animals to drink to achieve a probiotic effect. A fermented bacterial solution can be further dried and processed to prepare viable bacterial powder, and the viable bacterial powder is added into livestock and poultry feed as a solid microbial agent to feed the animals. The probiotic clostridium butyricum is applied to livestock and poultry breeding, the health condition of the cultured animals is effectively improved, the production performance is improved, the occurrence rate of enteritis and diarrhea of the livestock and poultry is reduced, the use of antibiotics is reduced, and support is provided for green breeding in the livestock and poultry industry.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, and relates to a probiotic Clostridium butyricum and its application. Background technique [0002] As a new type of antibiotic substitute, probiotics have many advantages such as no toxic side effects, no drug resistance, no residue, etc., and have been affirmed by the aquaculture industry. With the goal of building an environmentally friendly and ecologically healthy livestock and poultry breeding, the present invention discovers a strain of Clostridium butyricum with excellent performance through research, which can be used in livestock and poultry breeding to improve the growth performance of livestock and poultry, reduce the rate of diarrhea, and reduce antibiotics. Use to promote the sustainable development of livestock and poultry breeding, and ultimately realize the coordinated development of livestock and poultry breeding and the ecological environment. In the process ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/75A23K50/30A23K50/60A61K35/742A61P1/12C12R1/145
CPCA61K35/742A23K10/18A23K50/30A23K50/60A23K50/75A61P1/12C12N1/20C12N1/205C12R2001/145
Inventor 梁莉赵磊孔垂彬罗福玖魏焕巍周小媛周英俊
Owner 山东蔚蓝生物科技有限公司
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