A method for detecting aspirin content in blood plasma and a pretreatment kit
A medium aspirin and pretreatment technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complex sample pretreatment process, quantitative limit and precision not reaching the expected effect, low recovery rate, etc., and achieve easy clinical promotion Popularization, avoiding underdose, high accuracy effect
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Embodiment 1
[0044] Internal standard working solution (1) Preparation of
[0045] Take concentration 50μg / mL internal standard aspirin (aspirin -D4) 80μL was added to 4mL of methanol, mixed, final concentration of 1000ng / mL, as an internal standard working solution for use.
[0046] (2) Preparation of working solution Calibrator
[0047] Formulated with methanol 100μg / mL stock solution of aspirin, then six gradient prepared with blank plasma concentrations of 5000,2500,1250,625,312.5,156ng / mL, the working fluid as calibrators, take 300μL aliquots frozen in 1.5mL dry flask, placed in a freeze drier, after pre-freezing -55 ℃ Ramp 4h then, for the first time from the vacuum heating, heating facilitate sublimation process, can accelerate the drying rate, the dry powder prepared in -20 ℃ stored for use. In use, removed from the refrigerator and placed at room temperature for 15-20 min balance, and then were added 300μL of pure water, mixing spare.
[0048] (3) Preparation of solution quali...
Embodiment 2
[0065] (1) The lower limit of quantification
[0066] The SNR ≧ 10, when the repetitive Cv≤20%, accuracy 15% deviation as a quantitative index of the lower limit of determination standard, add aspirin 0.5ng / mL when the blank plasma, the test was repeated 10 times, sample preparation and after detecting machine, data analysis can meet the above criteria.
[0067] (2) Precision
[0068]Three horizontal controls and patients who took aspirin drugs were repeated 10 times, and 30 days were detected, 30 test data were obtained, and the repeatability CV was calculated, and the results were 2.2-5.4%.
[0069] (3) recovery rate
[0070] Add the same volume of different concentrations of aspirin standard solutions to blank plasma, so that the final concentration is 200, 500, 1000 ng / ml, respectively, and each concentration is repeatedly tested 3 times, and the three mean values are compared to the theoretical value, calculate the recovery rate, The result was 96 ± 3.1%.
[0071] (4) S...
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