Natural anti-aging mask and preparation method and application thereof
An anti-aging and facial mask technology, used in pharmaceutical formulations, cosmetic preparations, cosmetic preparations, etc., can solve the problems of dry cells, dull skin, damage to skin cells, etc., and achieve the effect of inhibiting skin aging and less irritation.
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Embodiment 1
[0030] 1. Preparation of Sargassum Polypeptide
[0031] (1) Grind the dried Sargassum to powder with liquid nitrogen;
[0032] (2) Add 10 times the volume of water, ultrasonically extract for 1 hour, filter, 3000r / min, centrifuge for 10min, remove the precipitate, and obtain a crude extract of Sargassum;
[0033] (3) Add 0.05 times cellulase to the crude extract of Sargassum, 50℃, pH 4.5, enzymatic hydrolysis for 2 hours;
[0034] (4) Maintain the temperature, adjust the pH to 6, add 0.05 times papain, and enzymatically hydrolyze for 3 hours;
[0035] (5) Reduce the temperature to 35°C, adjust the pH to 7, add 0.05 times trypsin, and enzymolyse for 3 hours;
[0036] (6) Raise the temperature to 90°C for 30 minutes, and then carry out enzyme inactivation treatment;
[0037] (7) The temperature was lowered to room temperature and centrifuged at 3000r / min for 30min to obtain the crude Sargassum polypeptide extract;
[0038] (8) Use a 500-2000Da nanofiltration membrane to filter the Sargassum ...
Embodiment 2
[0048] 1. Preparation of Sargassum Polypeptide
[0049] (1) Grind the dried Sargassum to powder with liquid nitrogen;
[0050] (2) Add 15 times the volume of water, ultrasonically extract for 2 hours, filter, 3000r / min, centrifuge for 20min, remove the precipitate, and obtain a crude extract of Sargassum;
[0051] (3) Add 0.1 times cellulase to the crude extract of Sargassum, 60°C, pH 5.5, enzymatic hydrolysis for 3 hours;
[0052] (4) Maintain the temperature, adjust the pH to 6.5, add 0.1 times papain, and enzymatically hydrolyze for 4 hours;
[0053] (5) Reduce the temperature to 40°C, adjust the pH to 8, add 0.2 times trypsin, and enzymolyse for 3-4 hours;
[0054] (6) Raise the temperature to 95°C for 30 minutes, and then carry out enzyme inactivation treatment;
[0055] (7) The temperature was lowered to room temperature and centrifuged at 3000r / min for 40min to obtain the crude Sargassum polypeptide extract;
[0056] (8) Use a 500-2000Da nanofiltration membrane to filter the Sargass...
Embodiment 3
[0066] 1. Preparation of Sargassum Polypeptide
[0067] (1) Grind the dried Sargassum to powder with liquid nitrogen;
[0068] (2) Add 10 times the volume of water, ultrasonically extract for 1 hour, filter, 3000r / min, centrifuge for 10min, remove the precipitate, and obtain a crude extract of Sargassum;
[0069] (3) Add 0.05 times cellulase to the crude extract of Sargassum, 50℃, pH 4.5, enzymatic hydrolysis for 2-3 hours;
[0070] (4) Maintain the temperature, adjust the pH to 6, add 0.05 times papain, and enzymatically hydrolyze for 3 hours;
[0071] (5) Reduce the temperature to 35°C, adjust the pH to 7, add 0.05 times trypsin, and enzymolyse for 3 hours;
[0072] (6) Raise the temperature to 90°C for 30 minutes, and then carry out enzyme inactivation treatment;
[0073] (7) The temperature was lowered to room temperature and centrifuged at 3000r / min for 30min to obtain the crude Sargassum polypeptide extract;
[0074] (8) Use a 500-2000Da nanofiltration membrane to filter the Sargassu...
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