Method for production of fish peptide with immobilized compound protease
A compound protease and protease technology, applied in the field of proteolysis, can solve the problems of low enzymatic hydrolysis efficiency and complicated enzymatic hydrolysis steps, and achieve the effects of simple preparation process, reduced production cost, and improved thermal stability
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Embodiment 1
[0026] The method for producing fish peptides by using immobilized composite protease in this embodiment includes the following steps:
[0027] 1) Take 0.5 kg of gelatin and 2 kg of sodium alginate and dissolve them in 100 kg of water at a temperature of 60°C to obtain a carrier solution.
[0028] 2) Add the protease mixture to the carrier solution in step 1). The protease mixture is a mixture of alkaline protease and trypsin. The mass ratio of alkaline protease, trypsin and carrier solution is 4:2:100, stir to dissolve , To obtain a composite protease solution.
[0029] 3) Drop the composite protease solution in step 2) onto CaCl 2 Immobilized in the solution into particles with a particle size of 0.5~2.5mm, CaCl 2 CaCl in solution 2 The mass ratio to water is 1.5:100, constant stirring during the immobilization process, the immobilization time is 30min, and the curing temperature is 25℃. After the immobilization is completed, filter with a 40-mesh sieve to obtain immobilized compos...
Embodiment 2
[0032] The method for producing fish peptides by using immobilized composite protease in this embodiment includes the following steps:
[0033] 1) Take 0.5 kg of gelatin and 2 kg of sodium alginate and dissolve them in 100 kg of water at a temperature of 60°C to obtain a carrier solution.
[0034] 2) Add the protease mixture to the carrier solution in step 1). The protease mixture is a mixture of alkaline protease and trypsin. The mass ratio of alkaline protease, trypsin and carrier solution is 4:2:100, stir to dissolve , To obtain a composite protease solution.
[0035] 3) Drop the composite protease solution in step 2) onto CaCl 2 Immobilized in the solution into particles with a particle size of 2~2.5mm, CaCl 2 CaCl in solution 2 The mass ratio to water is 2:100. Stir constantly during the immobilization process. The immobilization time is 50min and the immobilization temperature is 28℃. After the immobilization is completed, the immobilized composite enzyme particles are obtained...
Embodiment 3
[0038] The method for producing fish peptides by using immobilized composite protease in this embodiment includes the following steps:
[0039] 1) Take 1 kg of gelatin and 2 kg of sodium alginate and dissolve them in 100 kg of water at a temperature of 60° C. to obtain a carrier solution.
[0040] 2) Add the protease mixture to the carrier solution in step 1). The protease mixture is a mixture of alkaline protease and trypsin. The mass ratio of alkaline protease, trypsin and carrier solution is 6:4:100. Stir to dissolve , To obtain a composite protease solution.
[0041] 3) Drop the composite protease solution in step 2) onto CaCl 2 Immobilized in the solution into particles with a particle size of 0.5~1.5mm, CaCl 2 CaCl in solution 2 The mass ratio to water is 2.5:100. Stir constantly during the immobilization process. The immobilization time is 30min and the immobilization temperature is 22℃. After the immobilization is completed, filter with a 40-mesh sieve to obtain immobilized c...
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Abstract
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