Method for producing fish peptide by using immobilized composite protease
A compound protease and protease technology, applied in the field of proteolysis, can solve the problems of low enzymatic hydrolysis efficiency and complicated enzymatic hydrolysis steps, and achieve the effects of simple preparation process, reduced production cost, and improved thermal stability
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Embodiment 1
[0026] The method for producing fish peptides using immobilized composite protease of the present embodiment comprises the following steps:
[0027] 1) Take 0.5 kg of gelatin and 2 kg of sodium alginate and dissolve them in 100 kg of water at a temperature of 60° C. to obtain a carrier solution.
[0028] 2) Add protease mixed solution to the carrier solution in step 1), the protease mixed solution is a mixture of alkaline protease and trypsin, the mass ratio of alkaline protease, trypsin and carrier solution is 4:2:100, stir to dissolve , to obtain a compound protease solution.
[0029] 3) drop the compound protease solution in step 2) to CaCl 2 Immobilized in the solution into particles with a particle size of 0.5-2.5mm, CaCl 2 CaCl in solution 2 The mass ratio to water is 1.5:100, stirring continuously during the immobilization process, the immobilization time is 30min, and the curing temperature is 25°C. After the immobilization is completed, filter with a 40-mesh sieve ...
Embodiment 2
[0032] The method for producing fish peptides using immobilized composite protease of the present embodiment comprises the following steps:
[0033] 1) Take 0.5 kg of gelatin and 2 kg of sodium alginate and dissolve them in 100 kg of water at a temperature of 60° C. to obtain a carrier solution.
[0034] 2) Add protease mixed solution to the carrier solution in step 1), the protease mixed solution is a mixture of alkaline protease and trypsin, the mass ratio of alkaline protease, trypsin and carrier solution is 4:2:100, stir to dissolve , to obtain a compound protease solution.
[0035] 3) drop the compound protease solution in step 2) to CaCl 2 Immobilized into particles with a particle size of 2-2.5mm in the solution, CaCl 2 CaCl in solution 2 The mass ratio to water was 2:100. Stir continuously during the immobilization process. The immobilization time was 50 minutes. The immobilization temperature was 28°C. Second-rate.
[0036] 4) Fresh fish and water are beaten and ...
Embodiment 3
[0038] The method for producing fish peptides using immobilized composite protease of the present embodiment comprises the following steps:
[0039] 1) Take 1 kg of gelatin and 2 kg of sodium alginate and dissolve them in 100 kg of water at a temperature of 60° C. to obtain a carrier solution.
[0040] 2) Add protease mixed solution to the carrier solution in step 1), the protease mixed solution is a mixture of alkaline protease and trypsin, the mass ratio of alkaline protease, trypsin and carrier solution is 6:4:100, stir to dissolve , to obtain a compound protease solution.
[0041] 3) drop the compound protease solution in step 2) to CaCl 2 Immobilized in the solution into particles with a particle size of 0.5-1.5 mm, CaCl 2 CaCl in solution 2The mass ratio to water was 2.5:100, stirring continuously during the immobilization process, the immobilization time was 30min, and the immobilization temperature was 22°C. After the immobilization was completed, the immobilized co...
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