Preparation method of ultrathin APCS implant

Abstract

The invention discloses a preparation method of an ultrathin APCS implant and relates to the field of corneal endothelial sheets. The preparation method comprises the following steps: cleaning and dehydrating an acellular porcine corneal stroma, pre-cooling a freezing slicer to be lower than 20 DEG C, smearing an OCT embedding agent on a sample support, carrying out flattening, placing the dehydrated stroma on the OCT embedding agent, carrying out freezing, setting the slice thickness of the freezing slicer to be 20-60 microns, and carrying out cutting to obtain the ultrathin APCS implant slice . According to the method, the ultrathin APCS implant with the thickness of 20-60 microns is obtained, the integration of the implant and the eyes of a patient is effectively improved, the success rate of an operation is increased, the cost of the freezing slicer is low, the operation method is simple, the preparation cost of the implant can be effectively reduced, and the medical cost of the patient is reduced.

Description

technical field [0001] The invention relates to the field of corneal endothelial sheet preparation, in particular to a method for preparing an ultra-thin APCS graft. Background technique [0002] The corneal endothelium maintains the transparency and thickness of the cornea due to the barrier function formed by the tight junctions between the cells and the function of the sodium-potassium pump. , refractive surgery, early penetrating or endothelial keratoplasty, endothelial dystrophy, or certain disease stresses (such as diabetes or glaucoma) will cause a significant decrease in the density of corneal endothelial cells, once the density of corneal endothelial cells is less than 400 ~500 cells / mm 2 A cascade of interstitial edema, corneal opacity, bullous keratopathy, and visual loss occurs. [0003] With the advent of an aging population, the incidence of diabetes, glaucoma, and cataract is gradually increasing, and cataract surgery is becoming more and more common, and en...

AI Technical Summary

Problems solved by technology

[0005] At present, before using the porcine corneal stroma, it is usually necessary to cut the porcine corneal stroma into pieces before use. At present, femtosecond laser equipment is usually used for cutting: first cut the porcine cornea into corneal endothelial pieces with a thickness of not less than 100 μm, and then Corneal endothelial slices are decellularized for backup. However, due to the high price and complicated operation of femtosecond laser equipment, the cost of corneal endothelial slices is high, which increases the medical cost of patients. At the same time, corneal endothelial slices with a thickness of not less than 100 μm Due to the thicker thickness, the fusion with the patient's eye cells is poor after surgery, which easily leads to surgery failure