Method for improving vicilin functional properties
A vicilin and functional technology, which is applied in the field of protein modification, can solve the problems of poor functional properties of vicilin and limit the wide application of vicilin, and achieves improved hydrophobicity and thermodynamic properties, improved solubility, and good functional properties. Effect
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Embodiment 1
[0025] The commercially available raw pea flour was defatted with petroleum ether at a ratio of 1:5 (w / v) to obtain defatted pea flour. Extract defatted pea powder and deionized water at a ratio of 1:10 (w / v) for 2 hours, centrifuge at 8000 rpm for 10 minutes, discard the supernatant, and obtain a composite precipitate containing vicilin. The composite precipitate was extracted with deionized water containing 0.5M NaCl at a ratio of 1:5 (w / v) for 2 hours, centrifuged at 8000rpm for 10 minutes, the supernatant obtained was acid-precipitated, washed with water, refolded, dialyzed, and dried to obtain peas globulin. The vicilin was prepared into a protein solution with a concentration of 10% (w / v) with 20 mM phosphate buffer (pH 7.4). In the countercurrent ultrasonic modification process, the feed rate is 50mL / min, the water bath circulation system is used to control the reaction temperature at 20°C, the dual-frequency synchronous combination of 20kHz and 22kHz is used, the ultr...
Embodiment 2
[0029] The commercially available raw pea flour was defatted with petroleum ether at a ratio of 1:15 (w / v) to obtain defatted pea flour. Extract defatted pea powder and deionized water at a ratio of 1:10 (w / v) for 2 hours, centrifuge at 8000 rpm for 10 minutes, discard the supernatant, and obtain a composite precipitate containing vicilin. The composite precipitate was extracted with deionized water containing 0.5M NaCl at a ratio of 1:25 (w / v) for 2 hours, centrifuged at 8000 rpm for 10 minutes, and the supernatant obtained was acid-precipitated, washed with water, refolded, dialyzed, and dried to obtain peas globulin. The vicilin was prepared into a protein solution with a concentration of 10% (w / v) with 50 mM phosphate buffer (pH 7.4). In the countercurrent ultrasonic modification process, the feed rate is 100mL / min, the water bath circulation system is used to control the reaction temperature to 50°C, the dual-frequency synchronous combination of 33kHz and 40kHz is used, ...
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